HPLC Analysis of Ginsenoside Standards on Ascentis^® Express C18

技术

HPLC: suitable

试验参数

column: Ascentis Express C18, 15 cm x 4.6 mm I.D., 2.7 μm particles (53829-U)
mobile phase: [A]: water; [B]: acetonitrile
gradient: held at 25% for 1.5 min; 25 to 85% B in 12 min; held at 85% B for 1 min
flow rate: 1.0 mL/min
pressure: 3481 psi (240 bar)
column temp.: 60 °C
detector: UV, 205 nm
injection: 25 μL
sample: 50 μg/mL in water:methanol (82:18)

适用性

application for HPLC

应用

vitamins, nutraceuticals, and natural products