HPLC Analysis of Hemoglobin Variants on Discovery^® BIO PolyMA-WAX

技术

HPLC: suitable

试验参数

column: Discovery BIO PolyMA-WAX, 5 cm x 4.6 mm, 5 μm particles (59602-U)
mobile phase: [A] 10 mM Tris, pH 8.0 with acetic acid; [B] 10 mM Tris, 0.25 M KCl, pH 8.0 with acetic acid
gradient: 0 to 32% B in 20 min. (linear)
flow rate: 0.5 mL/min
column temp.: 35 °C
detector: UV, 280 nm
injection: 50 μg each Hb variants (A2, S and A0)

适用性

application for HPLC

应用

life science and biopharma