In Situ Hybridization Protocols 3rd ed.

This revised and updated edition of a recognized classic emphasizes tissue and cell in situ hybridization methods. Among the new techniques detailed are PNA probes for viral diagnostics, plant in situ hybridization, cell proliferation detection, and quantitation of in situ hybridization. There are also cutting-edge techniques for tissue microarrays, expanded embryology–developmental gene detection, and expanded cell culture. Derivative techniques presented include identification of transplanted cells, histones, nick-end labeling for apoptosis, the use of peptide nucleic acid probes, and in situ hybridization of plant specimens. The protocols include step-by-step laboratory instructions, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.

1. Treatment of Tissue Sections for In Situ Hybridization
2. Preparation of Template DNA and Labeling Techniques
3. In Situ Hybridization Using cRNA Probes: Isotopic and Nonisotopic Detection Methods
4. Tyramide Signal Amplification for DNA and mRNA In Situ Hybridization
5. Expression Analysis of Murine Genes Using In Situ Hybridization with Radioactive and Nonradioactively labeled RNA Probes
6. Nonradioactive In Situ Hybridization on Frozen Sections and Whole Mounts
7. In Situ Hybridization of Whole Mount Embryos
8. In Situ Detection of Epstein Barr Virus and Phenotype Determination of EBV Infected Cells
9. PNA-In Situ Hybridization Method for Detection of HIV-1 DNA in Virus Infected Cells and Subsequent Detection of Cellular and Viral Proteins
10. Electron Microscopy and In Situ Hybridization: Expression of P2Y₂ Receptor mRNA inthe Cerebellum
11. In Situ Hybridization Using Riboprobes on Free Floating Brain Section
12. An In Situ Hybridization Technique to Detect Low Abundance Slug mRNA in Adherent Cultured Cells
13. Identification of Transplanted Human Cells in Animal Tissue
14. In Situ Hybridization to Plant Tissues and Chromosomes
15. Histochemical Localization of Cell Proliferation Using In Situ Hybridization for Histone mRNA
16. Histochemical Localization of Apoptosis with In Situ Labeling of Fragmented DNA
17. The Use of Combined Immunohistochemical Labeling and In Situ Hybridization to Colocalize mRNA and Protein in Tissue Section
18. Semiquantitative In Situ Hybridization Using Radioactive Probes to Study Gene Expression in Motoneuron Populations
19. Quantitative In Situ Hybridization of Tissue Microarrays
Index