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安全信息

126615

Sigma-Aldrich

Albumin, Bovine Serum, 10% Aqueous Solution, Nuclease-Free

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别名:
Albumin, Bovine Serum, 10% Aqueous Solution, Nuclease-Free
CAS号:
UNSPSC代码:
12352202
NACRES:
NA.25

形式

liquid

质量水平

制造商/商品名称

Calbiochem®

储存条件

OK to freeze

杂质

≤0.02% Fatty acids
≤10 ppm Heavy metal
≤2.0% Ash

异质活性

Nuclease, none detected
Protease, none detected

运输

ambient

储存温度

2-8°C

一般描述

Purified chromatographically to reduce DNases and RNases to exceptionally low levels.
Useful for applications in which acetylated BSA is not desirable, such as in antibody dilution, DNA footprinting and gel shift assay, ELISA, enzyme assay, enzyme stabilization, immunoblotting, immunofluorescence, PCR, restriction enzyme reactions, RIA, probe-based diagnostics, radioactive quenching, and receptor binding studies. Purified chromatographically to reduce DNases and RNases to exceptionally low levels.
Useful for applications in which acetylated BSA is not desirable, such as in antibody dilution, DNA footprinting, and gel shift assay, ELISA, enzyme assay, enzyme stabilization, immunoblotting, immunofluorescence, PCR, restriction enzyme reactions, RIA, probe-based diagnostics, radioactive quenching, and receptor binding studies.

警告

Toxicity: Standard Handling (A)

外形

10% aqueous solution, 0.22 µm-filtered, pH 6.8-7.2.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

WGK

WGK 3

法规信息

监管及禁止进口产品

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Larry E Tetone et al.
Proceedings of the National Academy of Sciences of the United States of America, 114(7), E1081-E1090 (2017-02-01)
The secondary channel (SC) of multisubunit RNA polymerases (RNAPs) allows access to the active site and is a nexus for the regulation of transcription. Multiple regulatory proteins bind in the SC and reprogram the catalytic activity of RNAP, but the
Timothy T Harden et al.
Nature communications, 11(1), 448-448 (2020-01-25)
RNA polymerases (RNAPs) transcribe genes through a cycle of recruitment to promoter DNA, initiation, elongation, and termination. After termination, RNAP is thought to initiate the next round of transcription by detaching from DNA and rebinding a new promoter. Here we
Timothy T Harden et al.
Proceedings of the National Academy of Sciences of the United States of America, 113(3), 602-607 (2016-01-07)
Production of a messenger RNA proceeds through sequential stages of transcription initiation and transcript elongation and termination. During each of these stages, RNA polymerase (RNAP) function is regulated by RNAP-associated protein factors. In bacteria, RNAP-associated σ factors are strictly required
Alan J Simmons et al.
STAR protocols, 3(3), 101570-101570 (2022-07-27)
In droplet-based single-cell RNA-sequencing (scRNA-seq) experiments, cells, along with some of their surrounding buffer and ambient material, are encapsulated into droplets for mRNA capture and barcoding. This protocol details the steps for human gut tissue dissociation using cold active protease
Larry J Friedman et al.
Cell, 148(4), 679-689 (2012-02-22)
Understanding the pathway and kinetic mechanisms of transcription initiation is essential for quantitative understanding of gene regulation, but initiation is a multistep process, the features of which can be obscured in bulk analysis. We used a multiwavelength single-molecule fluorescence colocalization

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