一般描述
The REDExtract-N-Amp™ Seed PCR Kits provide all the reagents necessary to rapidly extract DNA from a wide variety of plant seeds and amplify targets of interest by direct PCR (polymerase chain reaction). A novel extraction method eliminates the need for long enzymatic digestions. The kit also includes a specially formulated hot start PCR reaction mix for amplification directly from the extract. The REDExtract-N-Amp PCR ReadyMix contains an inert dye that acts as a tracking dye and allows for convenient loading of PCR reactions onto agarose gels for analysis.
应用
REDExtract-N-Amp™ seed PCR Kit has been used for DNA extraction and to amplify hygromycin resistance gene.(hpt) by polymerase chain reaction (PCR).
特点和优势
- Perfect for quick genotyping results
- Rapid extraction of genomic DNA for PCR in 15 minutes
- No long enzymatic digestions
- Hot Start antibody for highly specific PCR amplification of genomic DNA
- Compatible with a wide variety of seed sources
- Extracts are stable at 4 °C for at least 6 months, allowing for multiple reassays
- Phenol/chloroform extraction or alcohol precipitation not required
- Column purification or centrifugation not required
原理
该试剂盒附带经验证的实验方案，可从玉米、小麦、向日葵、棉花、大豆、高粱、油菜和拟南芥种子中提取并扩增基因组DNA。可应要求提供其他实验方案。在常规操作步骤中，基因组DNA提取自研磨并在室温下的种子制备溶液和提取溶液中孵育10分钟的样本。将该样本加热至95 °C并持续3分钟，然后混入第三种溶液以便在PCR反应前中和抑制物。随后将一部分DNA提取物加入到含有引物和Extract-N-Amp PCR Reaction Mix（包含在试剂盒内）的PCR反应物中，PCR反应即开始。
其他说明
法律信息
依照美国专利号5,338,671和5,587,287和其他地区对应的专利，许可抗体用于体外研究用途。依照美国专利号5,436,134，终端用户已自Molecular Probes, Inc.处获得仅在研究和开发用途实时定量PCR中使用SYBR Green核酸染色剂的许可。SYBR为Molecular Probes, Inc.的注册商标。
本产品的使用受到如下一项或多项美国专利及其对应的境外专利声明保护：5,789,224, 5,618,711, 6,127,155以及与届满的美国专利号5,079,352对应的境外专利声明。购买本产品，即相当于依照境外的专利声明获得了一份受限制、不可转让的使用许可，即将此等数量的产品用于购买者内部的研究用途。我们未明确表示、暗示或以禁止反言的形式授予您任何其他专利声明下的权利、进行任何专利方法申请的权利、进行任何形式的商业服务的权利，包括但不限于出于收费或其他商业考虑而报告购买者的研究活动结果的权利。本产品仅适合于研究用途。如需用于Roche专利许可的诊断用途，需另外征得Roche许可。有关购买许可的更多信息，可联系Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA获取。
本产品的使用受到如下一项或多项美国专利及其对应的境外专利声明保护：5,789,224, 5,618,711, 6,127,155以及与届满的美国专利号5,079,352对应的境外专利声明。购买本产品，即相当于依照境外的专利声明获得了一份受限制、不可转让的使用许可，即将此等数量的产品用于购买者内部的研究用途。我们未明确表示、暗示或以禁止反言的形式授予您任何其他专利声明下的权利、进行任何专利方法申请的权利、进行任何形式的商业服务的权利，包括但不限于出于收费或其他商业考虑而报告购买者的研究活动结果的权利。本产品仅适合于研究用途。如需用于Roche专利许可的诊断用途，需另外征得Roche许可。有关购买许可的更多信息，可联系Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA获取。
Extract-N-Amp is a trademark of Sigma-Aldrich Co. LLC
REDExtract-N-Amp is a trademark of Sigma-Aldrich Co. LLC
仅试剂盒组分
产品编号
说明
- Seed Preparation Solution
相关产品
产品编号
说明
价格
警示用语：
Danger
危险分类
Aquatic Chronic 2 - Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - Skin Sens. 1
储存分类代码
10 - Combustible liquids
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
新产品
Sadiye Hayta et al.
Current protocols, 1(3), e58-e58 (2021-03-04)
Wheat, though a key crop plant with considerable influence on world food security, has nonetheless trailed behind other major cereals in the advancement of gene transformation technology for its improvement. New breeding technologies such as genome editing allow precise DNA
Marko Stojkovic et al.
Journal of separation science, 35(24), 3509-3513 (2012-12-12)
The use of CE with contactless conductivity detection for the determination of PCR products is demonstrated for the first time. The separation of specific length PCR products according to their size could be achieved using 5% PVP as a sieving
Angela Quarta et al.
FEMS microbiology letters, 259(1), 7-13 (2006-05-11)
The ability to rapidly distinguish trichothecene chemotypes in a given species/population of the genus Fusarium is important due to significant differences in the toxicity of these secondary metabolites. A multiplex PCR assay, based on primer pairs derived from the Tri3
商品
Extract-N-Amp™ PCR kits are the world’s first integrated extraction and amplification process for rapid blood, tissue or plant assays.
Simple DNA/RNA purification methods aid genome analysis from various sources, enhancing research efficiency.
简单的DNA和RNA纯法方法大幅推动了基因组和基因表达的分析和鉴定。人们需要快速、方便地从多种细胞来源分离DNA和RNA，包括哺乳动物、植物和细菌培养物来源的细胞和组织。
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
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