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WGA3

GenomePlex® WGA再扩增试剂盒

Reamplification of WGA product with minimal bias

别名:

GenomePlex ®全基因组扩增再扩增试剂盒, 全基因组再扩增试剂盒

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关于此项目

UNSPSC代码:
41121800
NACRES:
NA.55

主要文件

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质量水平

200

技术

whole genome amplification: suitable

运输

wet ice

储存温度

−20°C

相关类别

一般描述

GenomePlex® WGA 再扩增试剂盒采用了一种专利扩增方法，该方法基于基因组DNA的随机片段，并将得到的小片段转化为两侧有通用引物位点的PCR可扩增文库分子。 WGA是利用通用寡核苷酸引物对文库分子进行PCR扩增获得的。它允许用户在不干扰原始、有限的基因组DNA来源的情况下获得所需DNA的更高产量。基因组DNA可以再扩增5个周期，且具有最小的等位基因缺失，产生更高的产量。它为研究人员提供了基因组DNA的代表性~500倍扩增。

应用

GenomePlex ® WGA再扩增试剂盒已用于生产用地高辛-11-dUTP 标记的DNA探针（μBm-探针和μBsm-探针）。它也被用于重新扩增wga2扩增的基因组DNA，产生>10 μg gDNA，以进行微阵列杂交。

其他说明

此试剂盒中提供的通用引物序列被认为是专有的。

法律信息

本产品的使用受到如下一项或多项美国专利及其对应的境外专利声明保护： 5,789,224、5,618,711、6,127,155和美国境外的声明，对应于过期的美国专利号5,079,352。 购买本产品，即相当于依照境外的专利声明获得了一份受限制、不可转让的使用许可，即将此等数量的产品用于购买者′内部的研究用途。未通过明示、暗示或禁止反言形式授予购买者任何专利主张下的权利、执行任何专利方法的权利或开展任何形式的商业服务的权利，包括但不限于出于经济利益或其他商业考虑报告购买者′的活动结果。本品仅供研究使用。如需用于Roche专利许可的诊断用途，需另外征得Roche许可。有关购买许可的更多信息，可联系许可总监（Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA）获取。
GenomePlex is a registered trademark of Takara Bio USA, Inc.

仅试剂盒组分

产品编号
说明
  • Deoxynucleotide Mix, 10 mM

试剂盒组分也可单独购买

产品编号
说明
化学品安全说明书
  • D7295Deoxynucleotide Mix, 10 mM, Molecular Biology Reagent .2 mL化学品安全说明书

象形图

Health hazard
GHS08

警示用语：

Danger

危险声明

H334

预防措施声明

P261 - P284 - P501

危险分类

Resp. Sens. 1

储存分类代码

12 - Non Combustible Liquids

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

常规特殊物品
此项目有

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000473462
0000473462
0000441854
0000374640
SLCN5650

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访问文档库

Mirella L Meyer-Ficca et al.
Chromosoma, 122(4), 319-335 (2013-06-05)
The mammalian sperm nucleus is characterized by unique properties that are important for fertilization. Sperm DNA retains only small numbers of histones in distinct positions, and the majority of the genome is protamine associated, which allows for extreme condensation and
Yun Xia et al.
Frontiers in genetics, 10, 1364-1364 (2020-02-11)
Chromosomal rearrangements have long fascinated evolutionary biologists for being widely implicated in causing genetic differentiation. Suppressed recombination has been demonstrated in various species with inversion; however, there is controversy over whether such recombination suppression would facilitate divergence in reciprocal translocation
Churamani Khanal et al.
Plant disease, 103(8), 1835-1842 (2019-06-14)
This study employed single nucleotide polymorphisms (SNPs) to determine the genetic variability present in 26 isolates of Rotylenchulus reniformis from Louisiana, Mississippi, Arkansas, South Carolina, Georgia, Hawaii, and Alabama. Genomic DNA from reniform nematode was extracted and increased quantitatively using
Delimiting the origin of a B chromosome by FISH mapping, chromosome painting and DNA sequence analysis in Astyanax paranae (Teleostei, Characiformes)
Duilio M Z, et al.
PLoS ONE, 9(4), e94896-e94896 (2014)
Elizabeth P Murchison et al.
Cell, 148(4), 780-791 (2012-02-22)
The Tasmanian devil (Sarcophilus harrisii), the largest marsupial carnivore, is endangered due to a transmissible facial cancer spread by direct transfer of living cancer cells through biting. Here we describe the sequencing, assembly, and annotation of the Tasmanian devil genome
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商品

Whole Genome Amplification for Single Cell Biology

Whole genome amplification overcomes restrictions for single-cell genomic analyses with non-specific amplification.

实验方案

Buccal DNA Extraction & WGA Amplification Protocol

This protocol provides a simple and convenient method to isolate, amplify, and purify genomic DNA from buccal swabs. Buccal swabs are a convenient method of acquiring a DNA sample.

Plant DNA Extraction & WGA Amplification Protocol

GenomePlex® Whole Genome Amplification has been used to amplify genomic DNA from soybean, corn, tomato, purple coneflower, and ginseng.

GenomePlex® Single Cell Whole Genome Amplification Kit (WGA4) - Protocol

GenomePlex® is a Whole Genome Amplification (WGA) method that allows the researcher to generate a representative amplification of genomic DNA

Imprint® Chromatin Immunoprecipitation Kit (CHP1) Protocol

The Sigma Imprint Chromatin Immunoprecipitation Kit uses a plate based system to allow rapid ChIP assays in a high throughput format

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相关内容

GenomePlex Whole Genome Amplification Kit Protocol (WGA1)

GenomePlex is a Whole Genome Amplification (WGA) method that allows the researcher to generate a representative, 500-fold amplification of genomic DNA

Amplification of GenomeRepresentative DNA from Limited Sources with GenomePlex WGA Technology
GenomePlex Whole Genome Amplification

Automation Protocol

Whole Genome Amplification Product Listing

Our commitment to the Life Sciences continues through significant advances in PCR technology. Improvements to the key parameters of yield, length, specificity, and fidelity, as well as new applications for nucleic acid amplification form the basis of our research and development focus. We are dedicated to providing you with the newest technology as the field of Nucleic Acid Amplification evolves, making Sigma your preferred supplier for PCR products.

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