推荐产品
一般描述
应用
- 凝胶内蛋白消化及MALDI-TOF质谱分析
- 电喷雾电离质谱(ESI-MS)分析
- L. 植物杆菌的表面蛋白质组分析
- 凝胶过滤，超离心，旋转阴影电子显微镜
- 质谱
生化/生理作用
相关产品
警示用语：
Danger
危险分类
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
靶器官
Respiratory system
WGK
WGK 1
个人防护装备
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
法规信息
Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
How do I find price and availability?
There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 or view local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
What can I use to solubilize this Product T6567, Trypsin from porcine pancreas?
This product is soluble in 1mM HCL.
What is the package size of Product T6567, Trypsin from porcine pancreas?
Each vial content 20 ug of the product.
Is Product T6567, Trypsin from porcine pancreas, TPCK treated?
The product has been treated with TPCK to remove chymotryptic activity, further purified through affinity chromatography, and lyophilized, resulting in convenient use and highly specific cleavage. This information is on the product page under application.
How can we get Trypsin sequence information for Product T6567, Trypsin from porcine pancreas?
If you are looking for trypsin sequence information, you have to go to the NCBI Protein Data Bank.
How can we digest protein using Product T6567, Trypsin from porcine pancreas?
As per Sigma R and D the information is as follows: In order to efficiently digest a protein with trypsin, it must be denatured and the disulfide bonds modified by reduction and alkylation, or at least reduced. Many intact proteins are highly resistant to digestion with trypsin. If you do not want to reduce and alkylate, you can then just boil the protein with 5 mM DTT or 20 mM 2ME for 10 minutes, and then quickly cool on ice to denature the protein. This may result in a precipitate, but the trypsin will still digest the protein and it will clear within an hour or two. You can also dissolve the protein in 6 M guanidine-HCl or 8 M urea. Reduce and alkylate using the PROT-RA kit or other suitable method. Then they would have to dilute the solution to less than 2 M of either denaturant and then add the trypsin. This is the method that we use routinely in the lab.
My question is not addressed here, how can I contact Technical Service for assistance?
Ask a Scientist here.
商品
化学处理的重组胰蛋白酶在蛋白质组学和蛋白质表征分析中的应用评测
Evaluation of Recombinant, Chemically Treated Trypsin in Proteomics and Protein Characterization Assays
Pretreatment with Mucinase StcE increases glycopeptide identification from mucin samples, enhancing sample preparation efficiency for glycopeptide analysis.
The field of proteomics is continually looking for new ways to investigate protein dynamics within complex biological samples. Recently, many researchers have begun to use RNA interference (RNAi) as a method of manipulating protein levels within their samples, but the ability to accurately determine these protein amounts remains a challenge. Fortunately, over the past decade, the field of proteomics has witnessed significant advances in the area of mass spectrometry. These advances, both in instrumentation and methodology, are providing researchers with sensitive assays for both identification and quantification of proteins within complex samples. This discussion will highlight some of these methodologies, namely the use of Multiple Reaction Monitoring (MRM) and Protein-AQUA.
实验方案
本方案适用于使用Nα-苯甲酰基-L-精氨酸乙酯（BAEE）作为底物测定胰蛋白酶活性。本方案是基于连续分光光度速率测定（A253，光程= 1cm）方案：
Continuous spectrophotometric rate determination method using BAEE substrate measures trypsin activity, essential for enzyme characterization.
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