一般描述
三氯乙酸(TCA)是一种强酸。在饮用水pH值下,三氯乙酸呈现盐形式。
应用
三氯乙酸的应用示例:
- 用于吲哚胺2,3-双加氧酶(IDO)活性测定,水解N-甲酰犬尿氨酸并生成犬尿氨酸
- 用于人肺动脉平滑肌细胞(HPASMCs)增殖
- 在蛋白提取和定量时,处理研磨组织和沉淀蛋白
生化/生理作用
三氯乙酸(TCA)全身性无毒,长久以来被用作浅表脱皮试剂。可用作过氧化物酶体增殖剂。
三氯乙酸溶液一般用于沉淀蛋白。可用于通过定量沉淀法测定蛋白浓度。三氯乙酸也可用作显微脱钙剂和固定剂。
警示用语:
Danger
危险声明
危险分类
Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Corr. 1A - STOT SE 3
靶器官
Respiratory system
WGK
WGK 2
闪点(°F)
Not applicable
闪点(°C)
Not applicable
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实验方案
To measure glucose-6-phosphatase activity, the Taussky-Shorr method is used. This method is a spectrophotometric stop-rate determination assay that is measured at 660 nm.
本方法可用于以血红蛋白为底物的胃蛋白酶活性测定。这是一种分光光度速率终止测定法。
This procedure may be used for determination of Pepsin activity using hemoglobin as the substrate. It is a spectrophotometric stop rate determination.
This procedure may be used for the determination of Amyloglucosidase activity using starch as the substrate.
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