跳转至内容
Merck
CN

STR1

增强型禽类第一链合成试剂盒

Components for cDNA synthesis with enhanced AMV reverse transcriptase

别名:

eAMV-RT, 逆转录酶 cDNA 合成试剂盒

登录 查看组织和合同定价。

选择尺寸


关于此项目

NACRES:
NA.55
UNSPSC Code:
12352200
技术服务
需要帮助?我们经验丰富的科学家团队随时乐意为您服务。
让我们为您提供帮助
技术服务
需要帮助?我们经验丰富的科学家团队随时乐意为您服务。
让我们为您提供帮助

产品名称

增强型禽类第一链合成试剂盒, Components for cDNA synthesis with enhanced AMV reverse transcriptase

usage

sufficient for 50 reactions
 kit sufficient for 50 reactions

feature

dNTPs included
hotstart: no

technique(s)

RT-PCR: suitable

color

colorless

input

purified RNA

shipped in

dry ice

storage temp.

−20°C

Quality Level

General description

cDNA第一链合成试剂盒(增强型鸟类AMV-RT)采用高度纯化的鸟类成髓细胞性白细胞病毒逆转录酶(eAMV-RT),与标准AMV-RT或标准莫洛尼鼠类白血病病毒逆转录酶(MMLV-RT)相比,性能更加优越。 这种更加强大的eAMV逆转录酶可在高温(高达65℃)下转录棘手的二级结构,从总RNA或poly(A)+ RNA生成高质量的全长cDNA。
Sigma的cDNA第一链合成试剂盒(增强型禽类AMV-RT)还提供随机九核苷酸(9-mers)引物和anchored oligo(dT)23引物,以防有时基因特异性引物无法起效。anchored oligo由23个胸苷(T)残基和一个G、C或A残基(anchor/锚点)组成。锚点用于保证oligo(dT)引物结合在信使mRNA最起头处,不会留出一长段的未用序列。该试剂盒可产出适合各种下游应用的可靠cDNA,包括PCR。

Legal Information

Purchase of this product is accompanied by a limited license for use in the Polymerase Chain Reaction (PCR) process for research purposes only and in conjunction with a thermal cycler whose use in the automated performance of the PCR process is covered by an up-front license fee, either by payment to Applied Biosystems or as purchased, i.e., and authorized thermal cycler.

Other Notes

一单位酶可以多聚腺苷酸为模板,以12-18长度的oligo(dT)为引物,在10分钟内将1纳摩TMP整合进TCA-沉淀材料中。

Application

cDNA第一链合成试剂盒(增强型禽类AMV-RT)可用于cDNA合成,以便用于实时逆转录-聚合酶链式反应(RT-PCR)。

存储类别

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

法规信息

监管及禁止进口产品
此项目有

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

没有发现合适的版本?

如果您需要特殊版本,可通过批号或批次号查找具体证书。

已有该产品?

在文件库中查找您最近购买产品的文档。

访问文档库

Aerosol-administered α-tocopherol attenuates lung inflammation in rats given lipopolysaccharide intratracheally.
Experimental Lung Research, 31, 34510-34524 (2007)
Sigma-Aldrich Corporation's Life Science Quarterly. Hot Start RT-PCR results in improved performance of the enhanced Avian RT-PCR
Easlund, E., and Mueller, E.
Sigma data, 2-5 (2001)
Heat shock proteins expression during thermal risk exposure in the temperate xerothermic ant Formica cinerea
Slipinski P, et al.
Sociobiology, 62(3), 457-459 (2015)
JianFei Wang et al.
Journal of leukocyte biology, 82(6), 1554-1563 (2007-11-28)
Wound healing is a complex process involving the integrated actions of numerous cell types, soluble mediators, and ECM. Recently, a newly identified cell type, the fibrocyte, has been reported to contribute to wound healing and fibrotic conditions such as hypertrophic
María José Martín et al.
The Journal of biological chemistry, 282(47), 34510-34524 (2007-09-21)
Heme oxygenase-1 (HO-1), an inducible enzyme that metabolizes the heme group, is highly expressed in human Kaposi sarcoma lesions. Its expression is up-regulated by the G protein-coupled receptor from the Kaposi sarcoma-associated herpes virus (vGPCR). Although recent evidence shows that

商品

Challenges in gene expression analysis include mRNA stability, temporal transcription patterns, and mRNA-protein correlation, impacting accuracy.

分析基因表达的方法之一是测量基因的mRNA浓度。此类分析面临着若干挑战,例如不同转录本之间的半衰期不同、转录时间模式以及mRNA和蛋白质之间缺乏相关性。

实验方案

The 3’/5’ integrity assay identifies RNA degradation, crucial for large sample sets or less detectable degradation.

相关内容

PCR是分子生物学的核心技术,功能强大。可在体外高效快速地对不同来源的特定DNA或RNA序列进行酶扩增。

RT-qPCR detects specific targets with applications in gene expression and pathogen detection.

Polymerase chain reaction (PCR) is a technique for amplifying nucleic acid molecules and is commonly used in many applications, including RT-PCR, hot start PCR, end point PCR and more.

查看所有结果

我们的科学家团队拥有各种研究领域经验,包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

联系客户支持