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Sigma-Aldrich

增强型禽类第一链合成试剂盒

Components for cDNA synthesis with enhanced AMV reverse transcriptase

别名:

eAMV-RT, 逆转录酶 cDNA 合成试剂盒

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About This Item

UNSPSC代码:
12352200
NACRES:
NA.55

质量水平

200

用途

sufficient for 50 reactions
 kit sufficient for 50 reactions

特点

dNTPs included
hotstart: no

技术

RT-PCR: suitable

颜色

colorless

输入

purified RNA

运输

dry ice

储存温度

−20°C

一般描述

Sigma的cDNA第一链合成试剂盒（增强型禽类AMV-RT）还提供随机九核苷酸(9-mers)引物和anchored oligo(dT)23引物，以防有时基因特异性引物无法起效。anchored oligo由23个胸苷(T)残基和一个G、C或A残基（anchor/锚点）组成。锚点用于保证oligo(dT)引物结合在信使mRNA最起头处，不会留出一长段的未用序列。该试剂盒可产出适合各种下游应用的可靠cDNA，包括PCR。
cDNA第一链合成试剂盒（增强型鸟类AMV-RT）采用高度纯化的鸟类成髓细胞性白细胞病毒逆转录酶(eAMV-RT)，与标准AMV-RT或标准莫洛尼鼠类白血病病毒逆转录酶(MMLV-RT)相比，性能更加优越。 这种更加强大的eAMV逆转录酶可在高温（高达65℃）下转录棘手的二级结构，从总RNA或poly(A)+ RNA生成高质量的全长cDNA。

应用

cDNA第一链合成试剂盒（增强型禽类AMV-RT）可用于cDNA合成，以便用于实时逆转录-聚合酶链式反应(RT-PCR)。

单位定义

一单位酶可以多聚腺苷酸为模板，以12-18长度的oligo(dT)为引物，在10分钟内将1纳摩TMP整合进TCA-沉淀材料中。

法律信息

Purchase of this product is accompanied by a limited license for use in the Polymerase Chain Reaction (PCR) process for research purposes only and in conjunction with a thermal cycler whose use in the automated performance of the PCR process is covered by an up-front license fee, either by payment to Applied Biosystems or as purchased, i.e., and authorized thermal cycler.

仅试剂盒组分

产品编号
说明
  • Enhanced Avian Reverse Transcriptase 1000 U

试剂盒组分也可单独购买

产品编号
说明
化学品安全说明书
  • Deoxynucleotide mix 50 μL
  • O4387Anchored oligo (dT)23 100 μL化学品安全说明书
  • R7647Random nonamers 100 μL化学品安全说明书
  • O4387Ribonuclease inhibitor 50 μL化学品安全说明书
  • W1754PCR grade water 1.5 mL化学品安全说明书

储存分类代码

10 - Combustible liquids

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

监管及禁止进口产品

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
SLCQ9453
SLCP5967
SLCN0255
SLCH8162
SLCH2897

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Heat shock proteins expression during thermal risk exposure in the temperate xerothermic ant Formica cinerea
Slipinski P, et al.
Sociobiology, 62(3), 457-459 (2015)
Sigma-Aldrich Corporation's Life Science Quarterly. Hot Start RT-PCR results in improved performance of the enhanced Avian RT-PCR
Easlund, E., and Mueller, E.
Sigma data, 2-5 (2001)
Julio Carrion et al.
Journal of immunology (Baltimore, Md. : 1950), 189(6), 3178-3187 (2012-08-15)
The low-grade oral infection chronic periodontitis (CP) has been implicated in coronary artery disease risk, but the mechanisms are unclear. In this study, a pathophysiological role for blood dendritic cells (DCs) in systemic dissemination of oral mucosal pathogens to atherosclerotic
JianFei Wang et al.
Journal of leukocyte biology, 82(6), 1554-1563 (2007-11-28)
Wound healing is a complex process involving the integrated actions of numerous cell types, soluble mediators, and ECM. Recently, a newly identified cell type, the fibrocyte, has been reported to contribute to wound healing and fibrotic conditions such as hypertrophic
María José Martín et al.
The Journal of biological chemistry, 282(47), 34510-34524 (2007-09-21)
Heme oxygenase-1 (HO-1), an inducible enzyme that metabolizes the heme group, is highly expressed in human Kaposi sarcoma lesions. Its expression is up-regulated by the G protein-coupled receptor from the Kaposi sarcoma-associated herpes virus (vGPCR). Although recent evidence shows that
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逆转录

分析基因表达的方法之一是测量基因的mRNA浓度。此类分析面临着若干挑战，例如不同转录本之间的半衰期不同、转录时间模式以及mRNA和蛋白质之间缺乏相关性。

Reverse Transcription

Challenges in gene expression analysis include mRNA stability, temporal transcription patterns, and mRNA-protein correlation, impacting accuracy.

实验方案

The 3'/5' Assay for Analysis of RNA Integrity Protocol

The 3’/5’ integrity assay identifies RNA degradation, crucial for large sample sets or less detectable degradation.

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