推荐产品
等级
Molecular Biology
质量水平
表单
liquid
用途
0.1 mL sufficient for 100 RT-PCR reactions
浓度
50 μM in H2O
运输
wet ice
储存温度
−20°C
1 of 4
此商品 | O4387 | MBD6009 | 11004760001 |
|---|---|---|---|
| form liquid | form solution | form suspension | form - |
| usage 0.1 mL sufficient for 100 RT-PCR reactions | usage 0.1 mL sufficient for 100 RT-PCR reactions (as described in the Technical Bulletin for Product Codes HSRT100 and HSRT20) | usage - | usage sufficient for 50 labeling reactions |
| concentration 50 μM in H2O | concentration 70 μM in H2O | concentration - | concentration - |
| shipped in wet ice | shipped in wet ice | shipped in - | shipped in - |
| storage temp. −20°C | storage temp. −20°C | storage temp. 2-8°C | storage temp. −20°C |
一般描述
随机引物是由9个脱氧核苷酸组成的随机序列(9-mers)，可作为有或无poly(A)尾的mRNA的逆转录引物。随机引物也可作为通用的非特异性引物。
应用
随机九聚体适用于：
- 第一链 cDNA 合成
- 制备 cDNA 库（使用寡聚（dT）引物
- 制备用于杂交的已标记的DNA探针
特点和优势
- 当制备 cDNA 库或处理特异性引物不适合的情况时，可以将随机九聚体与锚定寡聚 (dT)23 引物（货号 O4387）组合。
- 在防止序列信息不完整或缺失，或特异性引物无效的情况下，随机九聚体为 cDNA 库提供了解决方案。
- 随机九聚体′ 在较高温度（高达 65°C）下的引发能力会降低，请确保它们不会干扰转录后的聚合酶链式反应 (PCR)。
- 随机九聚体也可用于制备杂交研究的标记DNA探针。随机九聚体可替代特异性逆转录(RT)引物，用于第一链合成、cDNA库构建和其他应用。
组分
随机引物以50 uM 水溶液形式提供。
其他说明
仅供实验室使用。不可用于药物、家庭或其他用途。
相关产品
产品编号
说明
价格
储存分类代码
12 - Non Combustible Liquids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
常规特殊物品
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
Sambrook, J., et al.
Molecular Cloning: A Laboratory Manual, 10-10 (1989)
P Goelet et al.
Proceedings of the National Academy of Sciences of the United States of America, 79(19), 5818-5822 (1982-10-01)
Oligonucleotide primers have been used to generate a cDNA library covering the entire tobacco mosaic virus (TMV) RNA sequence. Analysis of these clones has enabled us to complete the viral RNA sequence and to study its variability within a viral
Hsiao-Wen Lin et al.
Journal of neurochemistry, 111(3), 808-818 (2009-08-29)
Interleukin-6 (IL-6) is produced by neurons, astrocytes, and microglia, and elevated levels of IL-6 within the CNS have been documented in multiple neurological disorders including Alzheimer's disease, stroke, epilepsy, attention deficit disorder, cerebral palsy, and multiple sclerosis. Here, we sought
E M Brooks et al.
BioTechniques, 19(5), 806-812 (1995-11-01)
The secondary structure in mRNA is essential for many processes, but it can present a technical problem in making full-length cDNA with reverse transcriptases. Furthermore, different reverse transcriptases have differing abilities to transcribe through regions with secondary structure, which can
Ondrej Hajdusek et al.
Proceedings of the National Academy of Sciences of the United States of America, 106(4), 1033-1038 (2009-01-28)
Ticks are among the most important vectors of a wide range of human and animal diseases. During blood feeding, ticks are exposed to an enormous amount of free iron that must be appropriately used and detoxified. However, the mechanism of
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