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Merck
CN

R7647

Sigma-Aldrich

随机引物

for use as primers in cDNA synthesis, 50 μM in H2O

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别名:
Oligodeoxynucleotides
UNSPSC代码:
41105500
NACRES:
NA.31

等级

for molecular biology

质量水平

形式

liquid

用途

0.1 mL sufficient for 100 RT-PCR reactions

浓度

50 μM in H2O

运输

wet ice

储存温度

−20°C

一般描述

随机引物是由9个脱氧核苷酸组成的随机序列(9-mers),可作为有或无poly(A)尾的mRNA的逆转录引物。随机引物也可作为通用的非特异性引物。

应用

随机九聚体适用于:
  • 第一链 cDNA 合成
  • 制备 cDNA 库(使用寡聚(dT)引物
  • 制备用于杂交的已标记的DNA探针

特点和优势

  • 当制备 cDNA 库或处理特异性引物不适合的情况时,可以将随机九聚体与锚定寡聚 (dT)23 引物(货号 O4387)组合。
  • 在防止序列信息不完整或缺失,或特异性引物无效的情况下,随机九聚体为 cDNA 库提供了解决方案。
  • 随机九聚体′ 在较高温度(高达 65°C)下的引发能力会降低,请确保它们不会干扰转录后的聚合酶链式反应 (PCR)。
  • 随机九聚体也可用于制备杂交研究的标记DNA探针。随机九聚体可替代特异性逆转录(RT)引物,用于第一链合成、cDNA库构建和其他应用。

组分

随机引物以50 uM 水溶液形式提供。

其他说明

仅供实验室使用。不可用于药物、家庭或其他用途。

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

法规信息

常规特殊物品

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P Goelet et al.
Proceedings of the National Academy of Sciences of the United States of America, 79(19), 5818-5822 (1982-10-01)
Oligonucleotide primers have been used to generate a cDNA library covering the entire tobacco mosaic virus (TMV) RNA sequence. Analysis of these clones has enabled us to complete the viral RNA sequence and to study its variability within a viral
Sambrook, J., et al.
Molecular Cloning: A Laboratory Manual, 10-10 (1989)
Hsiao-Wen Lin et al.
Journal of neurochemistry, 111(3), 808-818 (2009-08-29)
Interleukin-6 (IL-6) is produced by neurons, astrocytes, and microglia, and elevated levels of IL-6 within the CNS have been documented in multiple neurological disorders including Alzheimer's disease, stroke, epilepsy, attention deficit disorder, cerebral palsy, and multiple sclerosis. Here, we sought
E M Brooks et al.
BioTechniques, 19(5), 806-812 (1995-11-01)
The secondary structure in mRNA is essential for many processes, but it can present a technical problem in making full-length cDNA with reverse transcriptases. Furthermore, different reverse transcriptases have differing abilities to transcribe through regions with secondary structure, which can
Karan J Abraham et al.
Nature, 585(7824), 298-302 (2020-07-17)
Proteins are manufactured by ribosomes-macromolecular complexes of protein and RNA molecules that are assembled within major nuclear compartments called nucleoli1,2. Existing models suggest that RNA polymerases I and III (Pol I and Pol III) are the only enzymes that directly

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