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此商品 | 244252 | 77234 | 311413 |
|---|---|---|---|
| concentration 0.01 M HClO4 in water (0.01N) | concentration - | concentration ≥60% (T) | concentration 60% |
| Quality Level 100 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| technique(s) ion chromatography: suitable | technique(s) toxicology assay: suitable | technique(s) - | technique(s) - |
一般描述
锚定寡核苷酸(dT)23引物用于启动聚(A)尾的mRNA，以进行cDNA合成。引物有23个胸腺嘧啶残基，在3′端有一个G、C或A残基（锚点）。这个锚点可确保寡核苷酸(dT)引物在信使的起点结合，并且没有很长的不可用序列区域。增强型AMV逆转录酶、模板RNA、引物的最佳浓度和扩增参数取决于所使用的系统，并且应该根据经验确定。
应用
锚定寡核苷酸23 引物由23个胸腺嘧啶核苷残基组成，并在3′端具有一个G、C或A残基（锚）。该锚定位点可确保寡核苷酸(dT)23引物与mRNA起始端结合，直接跳过无用的长序列区域。以poly(A)+ RNA为模板制备cDNA时，该锚定寡核苷酸 (dT)23 引物比标准寡核苷酸 (dT) 引物更具优势。
特点和优势
- 在制备cDNA文库过程中，当序列信息不完整或缺失或者特异性引物不合适时，可以使用Oligo(dT)23锚定引物和随机九聚体（产品编号R 7647）。
- 这些引物可以在cDNA第一链合成、cDNA文库构建和各种其他应用中作为逆转录引物的替代品。
- 转录后，oligo(dT)23锚定引物在高温（高达65 °C）下启动引物的能力降低，避免干扰PCR。
- 由poly(A)+ RNA产生cDNA时，oligo(dT)23锚定引物优于oligo(dT)标准引物。
其他说明
仅供实验室使用。不可用于药物、家庭或其他用途。
储存分类代码
10 - Combustible liquids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves
法规信息
常规特殊物品
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
Leila Azimi et al.
Gene, 576(1 Pt 1), 166-170 (2015-10-13)
Carbapenemase production causes multi antibiotics resistant in Gram-negative bacteria. A simple rapid and accurate phenotypic test for detection of Gram-negative carbapenemase-producing bacteria is useful for the treatment of infections. The aim of this study was to track the negative results
Fiona S McDonnell et al.
Journal of glaucoma, 25(10), e834-e842 (2016-10-18)
Glaucoma is an optic neuropathy that affects 60 million people worldwide. There is an underlying fibrosis associated with the lamina cribrosa (LC) in glaucoma. DNA methylation is well established in regulating fibrosis and may be a therapeutic target for glaucoma.
Christian Fork et al.
Journal of lipid research, 58(2), 386-392 (2016-12-04)
Nonsteroidal anti-inflammatory drugs are the most widely used medicine to treat pain and inflammation, and to inhibit platelet function. Understanding the expression regulation of enzymes of the prostanoid pathway is of great medical relevance. Histone acetylation crucially controls gene expression.
P Goelet et al.
Proceedings of the National Academy of Sciences of the United States of America, 79(19), 5818-5822 (1982-10-01)
Oligonucleotide primers have been used to generate a cDNA library covering the entire tobacco mosaic virus (TMV) RNA sequence. Analysis of these clones has enabled us to complete the viral RNA sequence and to study its variability within a viral
Sigma's New Enhanced Avian RT-PCR Kit.
Eastlund, E. and Song, K.
Sigma data, 1, 15-17 (2000)
商品
分析基因表达的方法之一是测量基因的mRNA浓度。此类分析面临着若干挑战，例如不同转录本之间的半衰期不同、转录时间模式以及mRNA和蛋白质之间缺乏相关性。
Challenges in gene expression analysis include mRNA stability, temporal transcription patterns, and mRNA-protein correlation, impacting accuracy.
实验方案
The 3’/5’ integrity assay identifies RNA degradation, crucial for large sample sets or less detectable degradation.
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