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SNC50

Sigma-Aldrich

mirPremier® microRNA分离试剂盒

1 sufficient for 50 preparations

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别名:
microRNA分离试剂盒
UNSPSC代码:
12352200
NACRES:
NA.52

质量水平

200

用途

sufficient for 50 preparations

技术

DNA extraction: suitable

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相关类别

一般描述

Sigma mirPremier microRNA分离试剂盒可以快速、有效地从不同的生物学来源样本，包括哺乳动物细胞培养物、动物组织、植物组织、微生物培养物中纯化和富集miRNA和其他小分子RNA，且无需进行任何有害的有机萃取。microRNA (miRNA) 属于小分子RNA，长度大约为21个核苷酸(nt)，通过各种方式调节着基因表达，包括翻译抑制、mRNA切割和脱腺苷化。此外，如果您关注信使RNA和其它大分子RNA，这种试剂盒还可用于分离总RNA。
.

应用

mirPremier® microRNA分离试剂盒已被用于：

  • 以来源于可食用植物葡萄的类外泌体纳米颗粒（EPDEN）提取含miRNA 的总RNA 。
  • 从S.sirkka 、S. napiecek和S. arctica中分离小分子RNA。
  • 从冷冻大鼠肝脏中分离miRNA用于miRNA 分析。

特点和优势

  • 旨在提高各种生物学来源的miRNA和其它小RNA分子的分离效率
  • 在30分钟内为下游应用实现快速而高效的miRNA提取和浓缩
  • 可以提取不含大RNA的高纯度miRNA
  • 不涉及危险的有机提取

法律信息

mirPremier is a registered trademark of Merck KGaA, Darmstadt, Germany

相关产品

产品编号
说明
价格

象形图

Exclamation mark
GHS07

警示用语：

Warning

危险分类

Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Irrit. 2 - Skin Irrit. 2

WGK

WGK 2

闪点(°F)

Not applicable

闪点(°C)

Not applicable

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ （Lot或Batch）字后找到。

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. Can I purify 18S and 28S large RNAs along with miRNAs with the mirPremier® microRNA Isolation Kit?  

    Large RNAs (18S and 28S) from the pellet fraction can be purified after transferring the microRNA-containing supernatant to a new tube by using the total RNA protocol or by phenol/chloroform extraction.

  6. Can the mirPremier® microRNA Isolation Kit be used to isolate small RNAs from purified total RNA?

    We have used to the kit to purify in vitro transcribed small RNAs with great success, but have not done so with purified total RNA. Here are the steps we would recommend trying with purified total RNA:1. Prepare a lysis mix with 0.7 vol. Small RNA Lysis Buffer (M1070) and 0.3 vol. Binding Solution (L8042).2. Add 500 ul of lysis mix with 50 ul total RNA and mix thoroughly.3. Spin at 14000 rpm for 5 min to precipitate large RNA.5. Transfer the supernatant to a new tube.6. Add 610 ul (1.1 vol.) 100% ethanol to the supernatant and mix well.7.Transfer the mixture to a binding column and spin 1 min to bind. Repeat the binding step with the remaining mixture.8. Wash the column first with 700 ul 100% ethanol, and then with ethanol-diluted wash Solution 2.9. Dry the column and elute small RNA.

  7. Has the mirPremier® microRNA Isolation Kit been tested on sperm cells?

    We have not tested mirPremier™ microRNA Isolation Kit  with sperm cells.

  8. How can I avoid co-purifying mRNAs and rRNAs when purifying small RNAs from gram negative bacteria using mirPremier® microRNA Isolation Kit?

    Too much residual medium or cell mass may lead to recovery of some large RNAs. It is critical to remove as much residual medium as possible by re-centrifuging the pellet. One can also test different ratios of the lysis mix (Small RNA Lysis Buffer and Binding Solution).

  9. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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Dietary phenolics exhibit hypolipidemic activity by changing lipid metabolism-related microRNA (miRNA) expression. Quercetin 3-O-rutinoside-7-O-α-l-rhamnosidase (quercetin 3-rut-7-rha), rutin and (-)-epicatechin are the main phenolics in lychee (Litchi chinensis Sonn.) pulp. A previous study reported that quercetin 3-rut-7-rha and rutin had hypolipidemic
Jon Bråte et al.
Current biology : CB, 28(20), 3288-3295 (2018-10-16)
The emergence of multicellular animals was associated with an increase in phenotypic complexity and with the acquisition of spatial cell differentiation and embryonic development. Paradoxically, this phenotypic transition was not paralleled by major changes in the underlying developmental toolkit and
Abdulla Abdulla Sabana et al.
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Genome-wide analysis of small RNAs identifies somatic embryogenesis- specific miRNAs and their targets and provides novel insights into the mechanisms governing somatic embryogenesis in coconut, a highly in vitro recalcitrant species. Coconut, a major plantation crop of the tropics is
Unicellular origin of the animal microRNA machinery
Br?te J, et al.
Current Biology, 28(20), 3288- 3295 (2018)
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