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Merck
CN
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安全信息

PP0201

Sigma-Aldrich

Glycoprofile™ II, Enzymatic In-Solution N-Deglycosylation Kit

sufficient for 20 samples

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UNSPSC代码:
12352202
NACRES:
NA.26

质量水平

200

储存温度

2-8°C

一般描述

含有足够进行至少20次反应所需的试剂，对于典型的糖蛋白，可处理样品范围为1~2 mg。

应用

GlycoProfile II Enzymatic In-Solution N-去糖基化试剂盒用于研究裂褶菌中N-乙酰-D-半乳糖胺（GalNAc）特异性凝集素的糖基化作用。
GlycoProfile™ II经过优化，可提供一种方便且可重复的从糖蛋白中去除N-连接聚糖的方法，与后续的MALDI-TOF质谱分析兼容，而不受任何反应成分的干扰。
Glycoprofile™ II, Enzymatic In-Solution N-去糖基化试剂盒已用于裂褶菌凝集素、拟南芥甘露聚糖酶、人脐静脉内皮细胞（HUVEC）蛋白裂解物和有机溶质转运蛋白Ostβ的去糖基化。

法律信息

GlycoProfile is a trademark of Sigma-Aldrich Co. LLC

仅试剂盒组分

产品编号
说明
  • 10× Reaction Buffer 1 mL/vial

试剂盒组分也可单独购买

产品编号
说明
化学品安全说明书
  • P7367PNGase F from Elizabethkingia meningoseptica, BioReagent, ≥95% (SDS-PAGE), for proteomics 50 U化学品安全说明书
  • R7884Ribonuclease B from bovine pancreas, BioReagent, ≥50 Kunitz units/mg protein, ≥80% (SDS-PAGE) .5 mg化学品安全说明书
  • O9882Octyl β-D-glucopyranoside, BioXtra, ≥98% (GC) 100 mg化学品安全说明书
  • M31482-Mercaptoethanol, for molecular biology, suitable for electrophoresis, suitable for cell culture, BioReagent, 99% (GC/titration) .9 mL化学品安全说明书

警示用语：

Danger

危险分类

Acute Tox. 2 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Repr. 2 - Resp. Sens. 1 - Skin Irrit. 2 - Skin Sens. 1 - STOT RE 2 Oral

靶器官

Liver,Heart

WGK

WGK 3

法规信息

危险化学品
常规特殊物品

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Christen Y L Yuen et al.
Plant & cell physiology, 58(6), 1103-1117 (2017-04-27)
Members of the protein disulfide isomerase (PDI)-C subfamily are chimeric proteins containing the thioredoxin (Trx) domain of PDIs, and the conserved N- and C-terminal Pfam domains of Erv41p/Erv46p-type cargo receptors. They are unique to plants and chromalveolates. The Arabidopsis genome
Enzymatic characterization of a glycoside hydrolase family 5 subfamily 7 (GH5\_7) mannanase from Arabidopsis thaliana
Wang Y, et al.
Planta, 239(3), 653-665 (2014)
Evaluation of soluble junctional adhesion molecule-A as a biomarker of human brain endothelial barrier breakdown
Haarmann A, et al.
PLoS ONE, 5(10), e13568-e13568 (2010)
Purification and characterization of an N-acetyl-D-galactosamine-specific lectin from the edible mushroom Schizophyllum commune
Chumkhunthod P, et al.
Biochim. Biophys. Acta Gen. Subj., 1760(3), 326-332 (2006)
Podjana Chumkhunthod et al.
Biochimica et biophysica acta, 1760(3), 326-332 (2006-03-02)
An N-acetyl-D-galactosamine (GalNAc)-specific lectin was purified from the edible mushroom, Schizophyllum commune, using affinity chromatography on a porcine stomach mucin (PSM)-Sepharose 4B column. Under reducing and non-reducing conditions, SDS-polyacrylamide gel electrophoresis gave a major band of 31.5 kDa. The Schizophyllum
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