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Merck
CN

PP0201

Sigma-Aldrich

Glycoprofile II, Enzymatic In-Solution N-Deglycosylation Kit

sufficient for 20 samples

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About This Item

UNSPSC代码:
12352202
NACRES:
NA.26

质量水平

储存温度

2-8°C

一般描述

含有足够进行至少20次反应所需的试剂,对于典型的糖蛋白,可处理样品范围为1~2 mg。

应用

GlycoProfile II Enzymatic In-Solution N-去糖基化试剂盒用于研究裂褶菌中N-乙酰-D-半乳糖胺(GalNAc)特异性凝集素的糖基化作用。
GlycoProfile II经过优化,可提供一种方便且可重复的从糖蛋白中去除N-连接聚糖的方法,与后续的MALDI-TOF质谱分析兼容,而不受任何反应成分的干扰。
Glycoprofile II, Enzymatic In-Solution N-去糖基化试剂盒已用于裂褶菌凝集素、拟南芥甘露聚糖酶、人脐静脉内皮细胞(HUVEC)蛋白裂解物和有机溶质转运蛋白Ostβ的去糖基化。

法律信息

GlycoProfile is a trademark of Sigma-Aldrich Co. LLC

仅试剂盒组分

产品编号
说明

  • 10× Reaction Buffer 1 mL/vial

试剂盒组分也可单独购买

产品编号
说明
化学品安全说明书

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  • M31482-Mercaptoethanol, for molecular biology, suitable for electrophoresis, suitable for cell culture, BioReagent, 99% (GC/titration) .9 mL化学品安全说明书

警示用语:

Danger

危险分类

Acute Tox. 2 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Repr. 2 - Resp. Sens. 1 - Skin Irrit. 2 - Skin Sens. 1 - STOT RE 2 Oral

靶器官

Liver,Heart

储存分类代码

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

法规信息

危险化学品
常规特殊物品

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分析证书(COA)

Lot/Batch Number

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访问文档库

Christen Y L Yuen et al.
Plant & cell physiology, 58(6), 1103-1117 (2017-04-27)
Members of the protein disulfide isomerase (PDI)-C subfamily are chimeric proteins containing the thioredoxin (Trx) domain of PDIs, and the conserved N- and C-terminal Pfam domains of Erv41p/Erv46p-type cargo receptors. They are unique to plants and chromalveolates. The Arabidopsis genome
Enzymatic characterization of a glycoside hydrolase family 5 subfamily 7 (GH5\_7) mannanase from Arabidopsis thaliana
Wang Y, et al.
Planta, 239(3), 653-665 (2014)
beta-Subunit of the Ostalpha-Ostbeta organic solute transporter is required not only for heterodimerization and trafficking but also for function
Christian WV, et al.
Test, 287(25), 21233-21243 (2012)
Elena Schwagerus et al.
Pflugers Archiv : European journal of physiology, 467(11), 2257-2273 (2015-02-14)
Using human airway epithelial cell lines (i.e. NCI-H441 and Calu-3) as well as human alveolar epithelial type I-like (ATI) cells in primary culture, we studied the contribution of the epithelial sodium channel δ-subunit (δ-ENaC) to transepithelial sodium transport in human
Purification and characterization of an N-acetyl-D-galactosamine-specific lectin from the edible mushroom Schizophyllum commune
Chumkhunthod P, et al.
Biochim. Biophys. Acta Gen. Subj., 1760(3), 326-332 (2006)

商品

Find deglycosylation kits for your research. Review the different features of deglycosylation kits for glycoproteins, enzymatic proteins, and native proteins.

实验方案

Explore mass spectrometry analysis of glycans for glycomic & glycoproteomic neutral & acidic glycan analysis. See a general mass spec glycan analysis procedure.

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