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质量水平
储存温度
2-8°C
一般描述
含有足够进行至少20次反应所需的试剂,对于典型的糖蛋白,可处理样品范围为1~2 mg。
应用
GlycoProfile II Enzymatic In-Solution N-去糖基化试剂盒用于研究裂褶菌中N-乙酰-D-半乳糖胺(GalNAc)特异性凝集素的糖基化作用。
GlycoProfile™ II经过优化,可提供一种方便且可重复的从糖蛋白中去除N-连接聚糖的方法,与后续的MALDI-TOF质谱分析兼容,而不受任何反应成分的干扰。
Glycoprofile™ II, Enzymatic In-Solution N-去糖基化试剂盒已用于裂褶菌凝集素、拟南芥甘露聚糖酶、人脐静脉内皮细胞(HUVEC)蛋白裂解物和有机溶质转运蛋白Ostβ的去糖基化。
法律信息
GlycoProfile is a trademark of Sigma-Aldrich Co. LLC
仅试剂盒组分
产品编号
说明
- 10× Reaction Buffer 1 mL/vial
试剂盒组分也可单独购买
产品编号
说明
化学品安全说明书
- P7367PNGase F from Elizabethkingia meningoseptica, BioReagent, ≥95% (SDS-PAGE), for proteomics 50 U化学品安全说明书
- R7884Ribonuclease B from bovine pancreas, BioReagent, ≥50 Kunitz units/mg protein, ≥80% (SDS-PAGE) .5 mg化学品安全说明书
- O9882Octyl β-D-glucopyranoside, BioXtra, ≥98% (GC) 100 mg化学品安全说明书
- M31482-Mercaptoethanol, for molecular biology, suitable for electrophoresis, suitable for cell culture, BioReagent, 99% (GC/titration) .9 mL化学品安全说明书
警示用语:
Danger
危险分类
Acute Tox. 2 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Repr. 2 - Resp. Sens. 1 - Skin Irrit. 2 - Skin Sens. 1 - STOT RE 2 Oral
靶器官
Liver,Heart
储存分类代码
6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
法规信息
危险化学品
常规特殊物品
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商品
Find deglycosylation kits for your research. Review the different features of deglycosylation kits for glycoproteins, enzymatic proteins, and native proteins.
实验方案
Explore mass spectrometry analysis of glycans for glycomic & glycoproteomic neutral & acidic glycan analysis. See a general mass spec glycan analysis procedure.
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