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安全信息

P7367

Sigma-Aldrich

糖苷酶F 来源于脑膜脓毒性伊丽莎白菌

BioReagent, ≥95% (SDS-PAGE), for proteomics

别名:

N-糖苷酶F, 糖苷酶F 来源于脑膜炎脓毒性黄杆菌, 糖苷酶F 来源于脑膜脓毒性金黄杆菌, 肽N-糖苷酶

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About This Item

CAS号:
83534-39-8
EC 号:
3.5.1.52 (BRENDA, IUBMB)
MDL编号:
MFCD00131222
UNSPSC代码:
12352204
NACRES:
NA.32

生物来源

bacterial (Elizabethkingia meningoseptica)

质量水平

200

偶联物

(N-linked)

等级

for proteomics

产品线

BioReagent

检测方案

≥95% (SDS-PAGE)

形式

powder

保质期

≥1 weeks at 2‑8 °C (for a reconstituted solution >500 units/ml)
≥1 yr at 2‑8 °C
Solution is stable for at least 3 freeze-thaw cycles

分子量

~36 kDa

浓度

≥300 units/mL
≥50 units/mL

最佳pH

~8.6

运输

wet ice

储存温度

2-8°C

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相关类别

一般描述

蛋白质组学级糖苷酶F经过从稀磷酸钾缓冲液中充分纯化和冻干，以生产出稳定的产品。 本品不含甘油和其他稳定剂，并且缓冲盐含量非常低。 这种高度纯化的材料非常适用于凝胶、溶液或印迹膜中糖蛋白或糖肽的N-连接去糖基化。

应用

来源于脑膜脓毒性黄杆菌（Elizabethkingia meningoseptica） 的PNGase F已被用于:
  • 寨卡20病毒E蛋白的去N-糖基化
  • 采用表达 CAR 的 COS 细胞评估柯萨奇病毒和腺病毒受体的糖基化
  • 验证 MHC 1 类多肽相关序列 A（MICA）的 N连接糖基化

适用于蛋白质组学和糖生物学；与MALDI-TOF MS分析相容
用于使蛋白质去糖基化。

生化/生理作用

从糖蛋白切割整个聚糖，前提是糖基化天冬酰胺部分在其氨基和羧基末端被多肽链取代。

单位定义

一个酶活性单位是指在37℃、pH 7.5条件下通过SDS-PAGE监测，在1分钟内催化1nM变性核糖核酸酶B释放N-连接寡糖所需的酶量。 糖苷酶F活性的一个Sigma单位相当于1 IUB毫单位。

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

法规信息

常规特殊物品

分析证书(COA)

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Terry Nguyen-Khuong et al.
Glycoconjugate journal, 35(6), 499-509 (2018-11-24)
Analysis of glycans via a porous graphitized carbon liquid chromatography (PGC-LC) coupled with electrospray ionization (tandem) mass spectrometry (ESI-MS(/MS)) is a powerful analytical method in the field of glycomics. Isobaric glycan structures can be identified reliably with the help of
Katherine J D Ashbourne Excoffon et al.
Journal of virology, 81(11), 5573-5578 (2007-03-23)
The coxsackievirus and adenovirus receptor (CAR) is both a viral receptor and homophilic adhesion protein. The extracellular portion of CAR consists of two immunoglobulin (Ig)-like domains, each with a consensus sequence for N-glycosylation. We used chemical, genetic, and biochemical studies
Lars Andresen et al.
Journal of immunology (Baltimore, Md. : 1950), 188(4), 1847-1855 (2012-01-10)
NKG2D ligand surface expression is important for immune recognition of stressed and neotransformed cells. In this study, we show that surface expression of MICA/B and other NKG2D ligands is dependent on N-linked glycosylation. The inhibitor of glycolysis and N-linked glycosylation
Giel Detienne et al.
Experimental gerontology, 60, 129-135 (2014-12-03)
Royalactin is a glycoprotein essential for the development of long-lived queen honeybees. Only larvae fed with royal jelly, containing royalactin, develop into queens. Royalactin plays a central role in this process by switching on the epidermal growth factor (EGF) receptor
Mikael B L Winkler et al.
Cell, 179(2), 485-497 (2019-09-24)
Niemann-Pick type C (NPC) proteins are essential for sterol homeostasis, believed to drive sterol integration into the lysosomal membrane before redistribution to other cellular membranes. Here, using a combination of crystallography, cryo-electron microscopy, and biochemical and in vivo studies on the
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