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安全信息

R7884

Sigma-Aldrich

核糖核酸酶B 来源于牛胰腺

BioReagent, ≥50 Kunitz units/mg protein, ≥80% (SDS-PAGE)

别名:

RNase B

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About This Item

CAS号:
9001-99-4
EC 号:
3.1.27.5 (BRENDA, IUBMB)
EC 号:
232-646-6
MDL编号:
MFCD00132184
UNSPSC代码:
12352204
NACRES:
NA.32

生物来源

bovine pancreas

质量水平

200

产品线

BioReagent

方案

≥80% (SDS-PAGE)

表单

powder

比活

≥50 Kunitz units/mg protein

浓度

≥60%

技术

cell based assay: suitable

适用性

suitable for molecular biology

应用

cell analysis

异质活性

protease ≤0.001 units/mg solid

储存温度

−20°C

SMILES字符串

[nH]1cncc1CC(NC(=O)CCN)C(=O)O

InChI

1S/C9H14N4O3/c10-2-1-8(14)13-7(9(15)16)3-6-4-11-5-12-6/h4-5,7H,1-3,10H2,(H,11,12)(H,13,14)(H,15,16)

InChI key

CQOVPNPJLQNMDC-UHFFFAOYSA-N

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应用

在细胞周期平台分析期间，牛胰腺核糖核酸酶B可用于消化RNA。

生化/生理作用

通过枯草杆菌蛋白酶消化天然RNase B（包含RNase B的氨基酸残基21-124）产生的天然RNase BS对PNGase F消化敏感。 牛胰腺RNase B的分子内N-聚糖具有伴侣蛋白的作用。 已有研究发现RNase B比RNase A起效更快，而RNase A在再生过程中易于聚集。Asn-寡糖（对应于RNase B的最主要糖链）的刺激作用表面RNase B的N-聚糖可促进大体积中间体转化为折叠的紧密物种。

包装

包装尺寸取决于蛋白质含量

制备说明

通过亲和层析纯化

抑制剂

产品编号
说明
价格

象形图

Health hazard
GHS08

警示用语：

Danger

危险声明

H334

预防措施声明

P261 - P284 - P501

危险分类

Resp. Sens. 1

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

法规信息

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分析证书(COA)

Lot/Batch Number
0000382117
0000365506
0000365507
0000327368
0000316973

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How should Ribonuclease B from bovine pancreas, Product R7884, be reconstituted?

    This product may be reconstituted directly in water at a concentration of 1 mg/mL.

  4. What is the extinction coefficient of Ribonuclease B from bovine pancreas (RNase B), Product R7884?

    The E1% for this enzyme is 8.0 when measured at 280 nm.

  5. What is the difference between RNase B, Ribonuclease B from bovine pancreas, Product R7884, and RNase A?

    The amino acid sequence for the two enzymes is the same. The difference between them is that RNase B is a glycoprotein and contains 6 mannose residues and 2 residues of N-acetylglucosamine.

  6. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  7. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  8. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Véronique Blanchard et al.
Biochemistry, 47(11), 3435-3446 (2008-02-26)
In glycoanalysis protocols, N-glycans from glycoproteins are most frequently released with peptide- N (4)-( N-acetyl-beta-glucosaminyl)asparagine amidase F (PNGase F). As the enzyme is an amidase, it cleaves the NH-CO linkage between the Asn side chain and the Asn-bound GlcNAc residue.
H Yamaguchi et al.
Journal of biochemistry, 120(3), 474-477 (1996-09-01)
This paper describes a chaperone-like function of the intramolecular N-glycans of bovine pancreatic RNase B. We studied air-oxidative regeneration from reductively denatured species of RNase B and its nonglycosylated form, RNase A. RNase B was reactivated much faster than RNase
Audra A Hargett et al.
Molecules (Basel, Switzerland), 26(14) (2021-07-25)
Protein glycosylation is important in many organisms for proper protein folding, signaling, cell adhesion, protein-protein interactions, and immune responses. Thus, effectively determining the extent of glycosylation in glycoprotein therapeutics is crucial. Up to now, characterizing protein glycosylation has been carried
Yang Xu et al.
iScience, 25(8), 104753-104753 (2022-08-10)
N-Acetylglucosamine (GlcNAc) is an essential monosaccharide required in almost all organisms. Fluorescent labeling of the peptidoglycan (PG) on N-acetylglucosamine has been poorly explored. Here, we report on the labeling of the PG with a bioorthogonal handle on the GlcNAc. We
Vijay Ramakrishnan et al.
American journal of hematology, 85(9), 675-686 (2010-07-24)
Interaction of myeloma cells with the bone marrow microenvironment is mediated in large part through different cytokines, especially VEGF and IL6. These cytokines, especially IL6, leads to upregulation of the JAK/STAT pathway in myeloma cell, contributing to increased proliferation, decreased
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