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P8125

Sigma-Aldrich

过氧化物酶 来源于辣根

Type I, essentially salt-free, lyophilized powder, ≥50 units/mg solid (using pyrogallol)

别名:

供体：过氧化氢氧化还原酶, 辣根过氧化物酶

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国内现货，预计发货时间April 24, 2025详情

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5000 UNITS
CN¥695.28
25000 UNITS
CN¥1,507.51
50000 UNITS
CN¥1,550.03
100000 UNITS
CN¥2,681.18
200000 UNITS
CN¥7,904.63

About This Item

CAS号:
9003-99-0
EC 号:
1.11.1.7 (BRENDA, IUBMB)
EC 号:
232-668-6
MDL编号:
MFCD00071339
UNSPSC代码:
12352204
eCl@ss:
32160410
NACRES:
NA.54

CN¥695.28

国内现货，预计发货时间April 24, 2025详情

类型

Type I

质量水平

200

表单

essentially salt-free, lyophilized powder

比活

≥50 units/mg solid (using pyrogallol)

分子量

~44 kDa

溶解性

0.1 M phosphate buffer: soluble (pH 6.0)
H2O: soluble

吸光度比值

RZ ≥1.0

储存温度

2-8°C

SMILES字符串

[O+H2]O[O-]

InChI

1S/H2O3/c1-3-2/h1-2H

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Peroxidase from horseradish Type XII, essentially salt-free, lyophilized powder, ≥250 units/mg solid (using pyrogallol)

P8415

Peroxidase from horseradish

Peroxidase from horseradish Highly stabilized, essentially salt-free, lyophilized powder, 200-300 units/mg solid (using pyrogallol)

P2088

Peroxidase from horseradish

form

essentially salt-free, lyophilized powder

form

essentially salt-free, lyophilized powder

form

essentially salt-free, lyophilized powder

form

essentially salt-free, lyophilized powder

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

mol wt

~44 kDa

mol wt

~44 kDa

mol wt

~44 kDa

mol wt

~44 kDa

solubility

0.1 M phosphate buffer: soluble (pH 6.0), H2O: soluble

solubility

0.1 M phosphate buffer: soluble 10 mg/mL, clear, orange to red (pH 6.0)

solubility

0.1 M phosphate buffer: soluble (pH 6.0), H2O: soluble

solubility

-

Quality Level

200

Quality Level

300

Quality Level

200

Quality Level

300

一般描述

辣根过氧化物酶是从辣根（Amoracia rusticana）中分离出来的，属于过氧化物酶的铁原卟啉基团。HRP是含有四个二硫键的单链多肽。它是一种含有18%碳水化合物的糖蛋白。碳水化合物由半乳糖、阿拉伯糖、木糖、岩藻糖、甘露糖、甘露糖胺和半乳糖胺组成，具体取决于特定同工酶。其分子量（约44 kDa）包括多肽链（33,890道尔顿）、氯化血红素加Ca2+ （约700道尔顿）和碳水化合物（约9,400道尔顿）。至少存在7种HRP同工酶。 辣根过氧化物酶同工酶的等电点范围为3.0-9.0。

应用

辣根过氧化物酶已用于：
  • 粪便淀粉分析[1]
  • 通过Trinder法测定血浆葡萄糖水平[2]
  • 孵育阿魏酰基阿拉伯糖基木聚糖组分并评估氧化偶联[3]
  • 检测糖蛋白[4]

生化/生理作用

HRP易与过氧化氢（H2O2）结合，所得[HRP-H2O2]配合物可氧化各种氢供体。最佳pH为6.0-6.5，酶在5.0-9.0的pH范围内最稳定。HRP可以通过几种不同的方法与抗体缀合，包括戊二醛、高碘酸盐氧化、通过二硫键以及通过氨基和巯基定向交联剂。它比酶标签β-半乳糖苷酶和碱性磷酸酶更小、更稳定。因此，它是最理想的标签。而且，糖基化使其非特异性结合较低。[5]它还用于测定溶液中的葡萄糖[6] 和过氧化物[7] 。已知叠氮化钠、氰化物、L-胱氨酸、重铬酸盐、亚乙基硫脲、羟胺、硫化物、钒酸盐、对氨基苯甲酸和Cd2+、Co2+、Cu2+、Fe3+、Mn2+、Ni2+、Pb2+离子可抑制酶活性。[8]
当与底物一起孵育时，辣根过氧化物酶可产生标记分子的有色荧光或发光衍生物，从而进行定量。

单位定义

一个连苯三酚单元将在20℃、pH 6.0条件下，在20秒内从连苯三酚形成1.0mg的红棓酚。

分析说明

RZ(Reinheitszahl)是指溶于去离子水中的酶(0.5–1.0 mg/ml)的吸光度比值A403/A275 。 它是血红素含量的量度，而不是酶活性。 即使是RZ值较高的制剂也可能具有较低的酶活性。

其他说明

有关过氧化物酶的更多信息，请访问 www.sigma-aldrich.com/enzymeexplorer。

象形图

Health hazard
GHS08

警示用语：

Danger

危险声明

H334

预防措施声明

P261 - P284 - P501

危险分类

Resp. Sens. 1

储存分类代码

11 - Combustible Solids

WGK

WGK 1

个人防护装备

Eyeshields, Gloves, type N95 (US)

法规信息

动植物源性产品
  • 技术规格说明书

    • 历史批次信息供参考:

    分析证书(COA)

    Lot/Batch Number
    0000313657
    0000313657
    0000400318
    0000400317
    0000400309

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    Acarbose enhances human colonic butyrate production
    Weaver G A, et al.
    The Journal of Nutrition, 127(5), 717-723 (1997)
    MBX-102/JNJ39659100, a Novel Non-TZD Selective Partial PPAR-gamma Agonist Lowers Triglyceride Independently of PPAR-alpha Activation
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    实验方案

    Spectrophotometric Determination of Reinheitszahl (RZ) for Peroxidase (EC 1.11.1.7)

    Reinheitszahl (RZ) is the ratio of absorbance due to hemin (A403, Soret region) to absorbance due to protein (A275).

    Enzymatic Assay of Peroxidase (EC 1.11.1.7) 2,2'-Azino-bis(3-Ethylbenzthiazoline-6-Sulfonic Acid) as a Substrate

    To standardize a procedure for the assay of Peroxidase using 2,2'-Azino-bis(3-Ethylbenzthiazoline-6-Sulfonic Acid) as a substrate.

    Enzymatic Assay of Peroxidase (EC 1.11.1.7)

    This procedure is for the determination of Peroxidase enzymatic activity using Pyrogallol as the substrate.

    过氧化物酶的酶学测定（EC 1.11.1.7）

    该实验程序用于使用连苯三酚作为底物测定过氧化物酶的酶活性。

    查看所有结果

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