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安全信息

P1867

Sigma-Aldrich

纤溶酶 来源于人类血浆

lyophilized powder, ≥2.0 units/mg protein

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别名:
纤溶酶
CAS号:
9001-90-5
EC 号:
3.4.21.7 (BRENDA, IUBMB)
EC 号:
232-640-3
MDL编号:
MFCD00131921
UNSPSC代码:
12352204
eCl@ss:
42030239
NACRES:
NA.54

形式

lyophilized powder

质量水平

200

比活

≥2.0 units/mg protein

杂质

HIV, Hepatitis B and Hepatitis C, tested negative

UniProt登记号

P00747

应用

diagnostic assay manufacturing

储存温度

−20°C

基因信息

human ... PLG(5340)

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一般描述

纤溶酶作为纤维蛋白溶解级联的关键酶起作用，并且在炎症过程中也是重要的。

应用

通过来自尿激酶活化的人血浆的亲和色谱法在研究中分离了纤溶酶和抑制剂之间的复合物。 它还被用于研究纤溶酶和胰凝乳蛋白酶激活人上皮钠通道（ENaC）的研究。

生化/生理作用

它会被α2-抗纤溶酶抑制并且阻断其与纤维蛋白的相互作用。 它消化细胞质蛋白α突触核蛋白的N-末端区域，防止其被周围细胞摄取。
纤溶酶在赖氨酸和精氨酸残基的羧基侧表现出优先的切割，其选择性高于胰蛋白酶。它可将聚合纤维蛋白转化为可溶性产物。
最适 pH：8.5
pH 7.5：约 40％ 的最大活性，约 50％ 的最大活性
最适温度：37°C，于 56°C 快速失活

包装

包装尺寸基于蛋白质含量

单位定义

一个单元将在 pH7.5，37℃ 下，每分钟从 D-Val-Leu-Lys-p-硝基苯胺生产 μmole 对硝基苯胺。

外形

含有磷酸钠、甘露醇和 NaCl 的冻干粉末。

免责声明

仅供研究使用。在法国，在用于科研目的时（包括进口和出口活动（《公共健康法》第L 1211-1条，第2节））该产品为管制品。购买者（即最终用户）需要获得《公共健康法》法第L 1211-1条规定的法国研究部的进口授权。订购本产品即表明您确认已经获得合适的进口授权。

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

常规特殊物品

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ （Lot或Batch）字后找到。

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. Can Product P1867, Plasmin from human plasma, be stored?

    Sigma has not evaluated the solution stability of plasmin solutions.It is best to prepare solutions fresh.

  6. Does Product P1867, Plasmin from human plasma, have a recommended retest date?

    If a product has a recommended retest date this would be on the Certificate of Analysis found at our website.  If no recommended retest date is found on the Certificate of Analysis, then the guarantee of quality is one year from the time of delivery for unopened bottles stored at the recommended temperature.

  7. Is there a conversion to International Units (I.U.) for Product P1867, Plasmin from human plasma?

    Sigma has not evaluated the activity of this enzyme in I.U., nor do we have a conversion factor.

  8. What is the molecular weight of Product P1867, Plasmin from human plasma?

    The molecular weight of this dimeric protein was reported to be 75,400 Daltons. Reference is: The Plasma Proteins, Volume 1, 2nd ed., Putnam, F.W., ed., p. 66 (1975).

  9. What is Product P1867, Plasmin from human plasma, soluble in?

    It is soluble in water.

  10. Can you provide a method of production for Product P1867 - Plasmin?

    Product P1867 - Plasmin from human plasma is from an outside supplier and the manufacturing process is considered proprietary.  The product is activated with Streptokinase and this enzyme is removed in the final steps of the purification.  The product is >95% by SDS PAGE. The product is >3% protein (the exact value will be on the lot specific Certificate of Analysis).  This material is lyophilized from 20 mM sodium phosphate (pH 7.4), containing 10 mg/ml D-mannitol and 10 mg/ml NaCl.  The lyophyllization buffer will contribute a significant amount of solids to the product.  The product package size is based on protein content.

  11. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

S Müllertz et al.
The Biochemical journal, 159(3), 545-553 (1976-12-01)
A complex between plasmin and an inhibitor was isolated by affinity chromatography from urokinase-activated human plasma. The complex did not react with antibodies against any of the known proteinase inhibitors in plasma. A rabbit antiserum against the complex was produced.
Efrat Shavit-Stein et al.
PloS one, 16(3), e0248431-e0248431 (2021-03-16)
Ischemic stroke is a common and debilitating disease with limited treatment options. Protease activated receptor 1 (PAR1) is a fundamental cell signaling mediator in the central nervous system (CNS). It can be activated by many proteases including thrombin and plasmin
Peter Stalmans et al.
Investigative ophthalmology & visual science, 54(10), 6620-6627 (2013-08-29)
To characterize the levels of active ocriplasmin over a period of ascending time points (range, 5 minutes to 7 days) from intravitreal injection of a 125-μg dose to sampling. During this 7-week controlled, open-label, phase 2 study, a single intravitreal
On the mechanism of the reaction between human alpha 2-antiplasmin and plasmin.
B Wiman et al.
The Journal of biological chemistry, 254(18), 9291-9297 (1979-09-25)
Dejun Yang et al.
Proceedings of the National Academy of Sciences of the United States of America, 118(33) (2021-08-13)
Idiopathic normal pressure hydrocephalus (iNPH) is a common neurological disorder that is characterized by enlarged cerebral ventricles, gait difficulty, incontinence, and dementia. iNPH usually develops after the sixth decade of life in previously asymptomatic individuals. We recently reported that loss-of-function
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实验方案

Enzymatic Assay of Plasmin with D-Val-Leu-Lys-p-Nitroanilide Dihydrochloride

Enzymatic Assay of Plasmin with D-Val-Leu-Lys-p-Nitroanilide Dihydrochloride

Enzymatic Assay of Plasmin with D-Val-Leu-Lys-p-Nitroanilide Dihydrochloride

Enzymatic Assay of Plasmin with D-Val-Leu-Lys-p-Nitroanilide Dihydrochloride

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