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安全信息

T4648

Sigma-Aldrich

凝血酶 来源于牛血浆

lyophilized powder, 40-300 NIH units/mg protein (biuret)

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别名:
IIa 因子
CAS号:
9002-04-4
EC 号:
3.4.21.5 (BRENDA, IUBMB)
EC 号:
232-648-7
MDL编号:
MFCD00166053
UNSPSC代码:
12352204
NACRES:
NA.54

生物来源

bovine plasma

质量水平

300

形式

lyophilized powder

比活

40-300 NIH units/mg protein (biuret)

分子量

heavy chain ~33 kDa
light chain ~5 kDa

组成

Protein, 40-60%

UniProt登记号

P00735

运输

wet ice

储存温度

−20°C

基因信息

cow ... F2(280685)

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一般描述

凝血酶是止血相关的最终凝血蛋白酶，对促凝血和抗凝血作用都有所促进。

应用

凝血酶可用于含有可接近的凝血酶识别位点以除去亲和标签的重组融合蛋白的位点特异性切割。凝血酶已用于一项对从脓毒性休克患者中持续性低凝状态进行评估的研究中。

生化/生理作用

丝氨酸蛋白酶，选择性断开纤维蛋白原中的精氨酸-甘氨酸键，形成纤维蛋白以及纤维蛋白肽 A 和 B。

单位定义

活性以NIH单位表示，通过与NIH凝血酶参考标准品直接对比确定。

外形

含有氯化钠和Tris-HCl，pH 7.0的冻干粉

制备说明

用牛脑促凝血酶原激酶进行激活。

分析说明

基于 Biggs 改性法的 NIH 分析过程使用 0.2mL 稀释血浆（由血浆与等量生理盐水混合制得）作为底物，以及 0.1mL 凝血酶样品（在含 1% 白蛋白缓冲液中保持稳定）。只使用 15-25 秒之间的凝血时间分析凝血酶浓度。
活性是通过与一种K批次的NIH凝血酶参考标准品进行直接比较而得到并以NIH单位进行表达的。

其他说明

查看更多关于 凝血酶的信息， 请访问 www.sigma-aldrich.com/enzymeexplorer。

象形图

Health hazardExclamation mark
GHS08,GHS07

警示用语：

Danger

危险分类

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

靶器官

Respiratory system

WGK

WGK 2

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

动植物源性产品

分析证书(COA)

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. What is the solubility of Product T4648, Thrombin from bovine plasma?

    Thrombin from bovine plasma is soluble in water at 10 mg/mL.

  6. What is the solution stability of Product T4648, Thrombin from bovine plasma?

    When stock solutions of thrombin from bovine plasma are prepared at a concentration of 100 units/mL in 0.1% (w/v) BSA, they are stable for one week at 0-5°C.  Since thrombin solutions adsorb to glass, it is recommended to aliquot the solutions in plastic tubes and store at -20°C for long term storage.

  7. What is the cross reactivity of Thrombin?

    Thrombin from any vertebrate species will clot the fibrinogen of virtually any other species.  Fibrinogen from any mammalian source will cross-react with thrombin from any mammalian source, but it is not well known how thrombin and fibrinogen from different animals differ in their sequence.  When any mammalian thrombin is injected into a different mammal, clotting will occur.

  8. What is the isoelectric point of Product T4648, Thrombin from bovine plasma?

    The isoelectric point of thrombin from bovine plasma is 7.05.

  9. How is the activity of Product T4648, Thrombin from bovine plasma, determined?

    The thrombin from bovine plasma activity is determined with a clotting assay (conversion of fibrinogen to fibrin) which measures alpha-thrombin and the amount of beta- and gamma-thrombin present is not measured. The assay of the clotting activity of thrombin from bovine plasma is performed at pH 7, utilizing a NIH thrombin reference standard.

  10. For thrombin, how do NIH units compare to International Units (IU)?

    One NIH unit of thrombin is equivalent to approximately 1.8 International Unit (IU) of thrombin.

  11. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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