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此商品 | 114529 | P3532 | PX0530 |
|---|---|---|---|
| form solid | form powder | form powder | form powder |
| storage temp. no temp limit | storage temp. room temp | storage temp. room temp | storage temp. 10-30°C |
| assay 98.0-102.0% (bromatometric, calculated on dried substance) | assay - | assay - | assay - |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level - |
| mp >300 °C | mp - | mp >300 °C | mp >300 °C |
| agency reag. Ph. Eur. | agency - | agency - | agency - |
应用
胶原酶已用于动脉组织的制备，用于晚期糖基化终产物 (AGE) 的研究。该酶还与其他蛋白酶一起使用，用于人体肿瘤、小鼠肾、人脑、肺上皮等多种组织的解聚。在肝脏和肾脏灌注研究、胰腺的消化和非实质肝细胞的分离中也有效。
生化/生理作用
组织溶梭菌包含两类胶原酶。梭菌胶原酶比哺乳动物胶原酶更高效。它在胶原三螺旋中的多个裂解位点进行裂解。梭菌胶原酶可参与气体坏疽中的细菌入侵。[4]
有效地从组织中释放细胞需要胶原酶和中性蛋白酶的共同作用。每摩尔酶用 4 克原子钙 (Ca2+) 激活胶原酶。一般认为培养滤液至少含有 7 种不同的蛋白酶，分子量范围为 68-130 kDa（最适用 pH：6.3-8.8）。该酶识别序列-R-Pro-8-X-Gly-Pro-R-，其中 X 是最常见的中性氨基酸。
每摩尔胶原蛋白酶被4g原子钙活化。 它被乙二醇-双（β-氨基乙基醚）-N，NN′,N′-四乙酸，β-巯基乙醇、谷胱甘肽、巯基乙酸和8-羟基喹啉抑制。
单位定义
一个胶原蛋白消化单元（CDU）从牛跟腱胶原蛋白中释放的肽链量和1.0 μ亮氨酸，5小时，pH7.4,37℃，在钙离子存在下的茚三酮颜色相同。一个 FALGPA水解单元每分钟，25℃，水解1.0 μmole 呋喃基丙烯酰-Leu-Gly-Pro-Ala。一个中性蛋白酶单元水解酪蛋白，产生颜色和1.0 μmole 酪氨酸每5小时，pH7.5,37℃下产生的相同。一个梭菌蛋白酶单元在DTT存在下，pH7.6,25℃，每分钟水解1.0 μmole BAEE。
制备说明
由 VII (C0773) 型制备。在 37℃ 下，将 0.05-0.1 mg 胶原酶溶于 1 mL 含 0.36 mM 氯化钙 (TESCA) 的 50 mM TES 缓冲液 (pH 7.4) 中，制备贮备液。
底物
警示用语：
Danger
危险分类
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
靶器官
Respiratory system
储存分类代码
11 - Combustible Solids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
法规信息
常规特殊物品
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
The proto-oncoprotein SYT (SS18) controls ATP release and regulates cyst formation by polarized MDCK cells
Chittezhath M, et al.
Experimental Cell Research, 314(19), 3551-3562 (2008)
Silke Proesmans et al.
Frontiers in medicine, 8, 744157-744157 (2021-11-09)
Introduction: [18F]-FDG PET is a widely used imaging modality that visualizes cellular glucose uptake and provides functional information on the metabolic state of different tissues in vivo. Various quantification methods can be used to evaluate glucose metabolism in the brain
Mohammed Alshareef et al.
International journal of molecular sciences, 23(6) (2022-03-26)
Germinal matrix hemorrhage (GMH) is a devastating disease of infancy that results in intraventricular hemorrhage, post-hemorrhagic hydrocephalus (PHH), periventricular leukomalacia, and neurocognitive deficits. There are no curative treatments and limited surgical options. We developed and characterized a mouse model of
Yasunori Asao et al.
Biochemical and biophysical research communications, 529(3), 590-595 (2020-08-02)
Intracerebral hemorrhage (ICH) is one of the most severe subtypes of stroke with high morbidity and mortality. Although a lot of drug discovery studies have been conducted, the drugs with satisfactory therapeutic effects for motor paralysis after ICH have yet
Andrea Comba et al.
Nature communications, 13(1), 3606-3606 (2022-06-25)
Intra-tumoral heterogeneity is a hallmark of glioblastoma that challenges treatment efficacy. However, the mechanisms that set up tumor heterogeneity and tumor cell migration remain poorly understood. Herein, we present a comprehensive spatiotemporal study that aligns distinctive intra-tumoral histopathological structures, oncostreams
实验方案
在测定胶原酶活性时，使用N-（3-[2-呋喃基]丙烯酰基）-Leu-Gly-Pro-Ala在345nm处进行连续分光光度法测定。胶原酶水解胶原肽键。
To measure collagenase activity, N-(3-[2-Furyl]acryloyl)-Leu-Gly-Pro-Ala is used in a continuous spectrophotometric rate determination at 345 nm. Collagenase hydrolyzes collagen peptide bonds.
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