表单
buffered aqueous solution
分子量
size 6787 bp
菌种筛选
kanamycin
复制起点
2Micron
pUC (500 copies)
肽切割
no cleavage
肽标签位置
N-terminal
启动子
Promoter name: TEF1
Promoter activity: constitutive
Promoter type: yeast
报告基因
none
分泌信号
alpha factor FL
运输
ambient
储存温度
−20°C
酵母筛选
uracil
一般描述
This plasmid is designed to express tagged proteins in yeast cells (Saccharomyces cerevisiae). The plasmid contains an auxotrophic Uracil selection expression cassette (URA3) that allows for the positive selection of yeast that are deficient in the URA3 gene (YEL021W). This is typically achieved by growing the yeast in minimal media that is reconstituted with the essential amino acids and nucleotides but excluding Uracil.
About the Peptide Tag:This plasmid contains an Invertase (Ints) secretory signal peptide (SP) to allow proteins to be exported from the cytosol. During translocation from the cytosol the signal peptide is removed from the protein by endogenous proteases.
Promoter Expression Level: This plasmid contains the Yeast Elongation Factor Alpha promoter (TEF1). This is the strongest of the yeast promoters that we sell. It is a constitutive promoter and requires no induction. If you are interested in weaker promoters levels than we also stock plasmids that contain the following promoters in order of decreasing strength TPI (strong), ADHI (medium), STE5 (weak). We also stock Galactose inducible promoter plasmids if inducible expression is required. Please contact us for further information.
About the Peptide Tag:This plasmid contains an Invertase (Ints) secretory signal peptide (SP) to allow proteins to be exported from the cytosol. During translocation from the cytosol the signal peptide is removed from the protein by endogenous proteases.
Promoter Expression Level: This plasmid contains the Yeast Elongation Factor Alpha promoter (TEF1). This is the strongest of the yeast promoters that we sell. It is a constitutive promoter and requires no induction. If you are interested in weaker promoters levels than we also stock plasmids that contain the following promoters in order of decreasing strength TPI (strong), ADHI (medium), STE5 (weak). We also stock Galactose inducible promoter plasmids if inducible expression is required. Please contact us for further information.
序列
To view sequence information for this product, please visit the product page
分析说明
To view the Certificate of Analysis for this product, please visit www.oxgene.com
相关产品
产品编号
说明
价格
储存分类代码
12 - Non Combustible Liquids
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
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