等级
for molecular biology
质量水平
无菌性
non-sterile
形式
powder
组成
Casamino Acids, 1 g/L
MgSO4, 0.98 g/L
NaCL, 5 g/L
Tryptone, 10 g/L
Yeast extract, 5 g/L
技术
microbiological culture: suitable
pH值(酸碱度)
6.8-7.2(2.2% solution)
应用
food and beverages
microbiology
储存温度
room temp
适用性
nonselective for Escherichia coli
nonselective for coliforms
一般描述
NZCYM 是用于培养 大肠杆菌 和 λ 噬菌体的微生物生长培养基。这种营养丰富的微生物肉汤含有多肽、氨基酸和水溶性维生素,呈低盐配方。
应用
NZCYM 肉汤可用于非选择性培养lambda, MS2, T7, CLP-P3, Phi29, 和 Goe1 噬菌体, 以及用于克隆,DNA质粒生产和重组蛋白生产的 大肠杆菌 菌株。 当加入适当抗生素时,也适用于选择性培养。
特点和优势
NZCYM 粉末:
- 标准配方
- 方便的包装规格
- 一种预算较低的液体替代品
重悬
通过搅拌使22 g粉末悬浮在1L水中。在121℃下高压灭菌15分钟。在加入添加物,例如抗生素(如果需要),之前请先冷却。
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
BMC microbiology, 7, 90-90 (2007-10-20)
The predominant food borne pathogen in the western world today is Campylobacter. Campylobacter specific bacteriophages (phages) have been proposed as an alternative agent for reducing the burden of Campylobacter in broilers. One concern in relation to phage biocontrol is the
BMC microbiology, 10, 232-232 (2010-09-03)
Poultry meat is one of the most important sources of human campylobacteriosis, an acute bacterial enteritis which is a major problem worldwide. Campylobacter coli and Campylobacter jejuni are the most common Campylobacter species associated with this disease. These pathogens live
Applied and environmental microbiology, 72(7), 4638-4647 (2006-07-06)
This study characterizes the interaction between Campylobacter jejuni and the 16 phages used in the United Kingdom typing scheme by screening spontaneous mutants of the phage-type strains and transposon mutants of the sequenced strain NCTC 11168. We show that the
Bioreagents for Cloning
Sigma data, 2-2 (2011)
The Journal of biological chemistry, 277(19), 17062-17071 (2002-02-14)
Trypanosoma cruzi glutathione-dependent peroxidase I (TcGPXI) can reduce fatty acid, phospholipid, and short chain organic hydroperoxides utilizing a novel redox cycle in which enzyme activity is linked to the reduction of trypanothione, a parasite-specific thiol, by glutathione. Here we show
实验方案
在从小型培养到发酵级的不同规模下培养大肠杆菌。包括了用于启动起始培养物、悬浮细胞、单一培养物和平板噬菌体M13的实验方案。
General protocols for growth of competent cells in microbial medium.
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