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一般描述
溶菌酶检测试剂盒提供检测溶菌酶活性存在的即用型试剂。该检测溶菌酶活性的简单方法使用 溶壁微球菌细胞作为底物。溶菌酶活性导致溶壁微球菌细胞裂解。在孵育溶菌酶样品和底物期间，通过监测 450 nm 处的吸光度降低来跟踪反应。
应用
溶菌酶活性试剂盒已用于测定天然溶菌酶、鸡蛋清纯化的溶菌酶和毛果芸香碱刺激唾液中溶菌酶的催化活性。
生化/生理作用
溶菌酶水解裂解 N -乙酰-D-氨基葡萄糖和 N -乙酰胞壁酸残基（GlcNAcβ1-4Mur）的粘多糖和相应的肽聚糖的聚糖骨架（后者是通过肽链连接或交联的糖胺聚糖）。溶菌酶能有效地破坏细菌的细胞壁。
储存及稳定性
反应缓冲液在 2–8°C 下至少可稳定 2 年。
溶菌酶（目录号 L6876）可在 –20℃ 下长期储存，并保持活性 4 年。
溶壁微球菌细胞（目录号 M3770）应在-20°C 条件下长期储存。
溶菌酶（目录号 L6876）可在 –20℃ 下长期储存，并保持活性 4 年。
溶壁微球菌细胞（目录号 M3770）应在-20°C 条件下长期储存。
警示用语：
Danger
危险声明
预防措施声明
危险分类
Resp. Sens. 1
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
高风险级别生物产品--病毒，疫苗等
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