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应用
溶壁微球菌ATCC号4698，已被用于一项评估中空纤维超滤分离溶菌酶的研究中。 它也被用于研究可生物降解微粒中蛋白质药物的封装研究。
从溶壁微球菌培养物中收获的溶菌酶裂解物被附着在琼脂糖凝胶上并用于亲和层析以分离各种溶菌酶。
生化/生理作用
藤黄微球菌(Micrococcus luetus)是一种革兰氏阳性菌，可通过释放水不溶性黄色素加以鉴定。这种细菌生长需要琥珀酸，并易被产酶溶杆菌(Lysobacter enzymogenes)通过β-水解金属内肽酶(β-lytic metalloendopeptidase)裂解。它的膜中含有一种酶，可以异戊二烯焦磷酸为供体进行异戊二烯化反应。藤黄微球菌可用于cis-异戊烯基转移酶的基因克隆。
质量
含多核苷酸磷酸化酶。
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
高风险级别生物产品--病毒，疫苗等
Generation of a reference transcriptome for evaluating rainbow trout responses to various stressors.
Cecilia C Sánchez et al.
BMC genomics, 12, 626-626 (2011-12-23)
Fish under intensive culture conditions are exposed to a variety of acute and chronic stressors, including high rearing densities, sub-optimal water quality, and severe thermal fluctuations. Such stressors are inherent in aquaculture production and can induce physiological responses with adverse
Tania Jauslin et al.
mBio, 12(1) (2021-02-18)
Ingestion and killing of bacteria by phagocytic cells protect the human body against infections. While many mechanisms have been proposed to account for bacterial killing in phagosomes, their relative importance, redundancy, and specificity remain unclear. In this study, we used
Ingrid Bourgeois et al.
FEMS microbiology letters, 290(1), 105-113 (2008-11-26)
The nucleotide sequence of atlL, a gene encoding a putative Staphylococcus lugdunensis peptidoglycan hydrolase, was determined using degenerate consensus PCR and genome walking. This 3837-bp gene encodes a protein, AtlL, that appears as a putative bifunctional autolysin with a 29-amino
Ghosh et al.
Biochemical engineering journal, 6(1), 19-24 (2000-07-26)
This paper discusses the purification of lysozyme from chicken egg white using hollow-fibre ultrafiltration (30kDa MWCO, polysulphone membrane). Lysozyme is preferentially transmitted through the membrane while the membrane largely retains other egg white proteins. Improvement in system hydrodynamics resulted in
Jingwei Xie et al.
Journal of colloid and interface science, 317(2), 469-476 (2007-10-20)
A co-axial electrospray process was developed to encapsulate protein-based drugs in biodegradable polymeric microparticles eliminating the key problem faced by other conventional methods of protein encapsulation--the primary emulsion being a major cause for protein denaturation and aggregation. Bovine serum albumin
实验方案
To measure achromopeptidase activity, this procedure uses bacterial cells and a turbidimetric rate assay. Turbidity is measured at 600 nm using a spectrophotometer.
This enzymatic rate determination may be used for Lysozyme products. It is not to be used to assay recombinant or insoluble Lysozyme on agarose.
该酶速率测定可用于溶菌酶产物。它不适用于在测定在琼脂糖上的重组型或不溶性溶菌酶。
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