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安全信息

L9234

Sigma-Aldrich

LB Broth (Lennox)

Liquid microbial growth medium

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UNSPSC代码:
12352200

等级

for molecular biology

质量水平

无菌性

sterile; 0.2 μm filtered

形式

liquid

适用性

suitable for nonselective for Escherichia coli and Coliforms

储存温度

room temp

一般描述

Liquid media is also termed as broth. It has nutrients dissolved in water. Luria-Bertani broth (LB broth) is used in naphthalene degradation experiments.

应用

LB Broth has been used to culture P. aeruginosa.
Suitable for non-selective cultivation of E. coli strains for cloning, DNA plasmid production and production of recombinant proteins. Also suitable for selective cultivation when appropriate antibiotics are added.

生化/生理作用

LB Lennox media is a highly-referenced microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins, and carbohydrates.

特点和优势

LB Lennox media provides:
  • Filter-sterilized, ready-to-use format
  • Convenient package size
  • Standard formulation

组分

10 g/L Tryptone
5.0 g/L Yeast Extract
5.0 g/L NaCl

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

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Understanding Microbes: A Laboratory Textbook for Microbiology (1989)
Pseudomonas toxin pyocyanin triggers autophagy: Implications for pathoadaptive mutations
Yang ZS, et al.
Autophagy, 12(6), 1015-1028 (2016)
Su-Yun Chang et al.
Water environment research : a research publication of the Water Environment Federation, 87(1), 61-67 (2015-01-30)
Luria-Bertani broth and acetone were usually used in naphthalene degradation experiments as nutrient and solvent. However, their effect on the degradation was seldom mentioned. In this work, we investigated the effect of LB, naphthalene concentration, and acetone on the degradation

实验方案

准备起始培养物

Initiating a Starter Culture

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