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Merck
CN

L4395

Sigma-Aldrich

脂质体试剂盒:用于制备脂质体的脂质混合物

lyophilized powder

别名:

cholesterol, L-α-phosphatidylcholine and stearylamine kit

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About This Item

EC 号:
UNSPSC代码:
12352211
NACRES:
NA.25

生物来源

egg yolk

质量水平

形式

lyophilized powder

组成

Cholesterol, 9 μmol/package
L-α-Phosphatidylcholine (egg yolk), 63 μmol/package
Stearylamine, 18 μmol/package

官能团

carboxylic acid

运输

ambient

储存温度

−20°C

应用

脂质体试剂盒用于制备脂质体。

生化/生理作用

脂质体可用于不同的应用,例如药物递送、诊断、膜模型、疫苗接种佐剂等。这种脂质混合物可用于封装各种低分子量、中等分子量和高分子量的亲水和两亲分子(包括肽、蛋白质以及寡核苷酸和多核苷酸)。
脂质体试剂盒:用于制备脂质体的脂质混合物含有胆固醇;L-α-磷脂酰胆碱和硬脂胺。 脂质体试剂盒可用于制备阳离子(带正电荷)脂质体,用于将材料掺入细胞中。

相关产品

象形图

CorrosionEnvironment

警示用语:

Danger

危险声明

危险分类

Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable


分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ (Lot或Batch)字后找到。

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. I have purchased Product L4395, Liposome Kit.  Is there a difference between positive and negative liposomes?

    The choice is dependent upon what is being encapsulated and how it is being used.  A good reference discussing this question is Ogiso, T., et al., Effect of positively and negatively charged liposomes on skin permeation of drugs. J. Drug Target, 9(1), 49-59 (2001).

  4. How is Product L4395, Liposome Kit, used?

    The Liposomes of a large multilamellar structure type are the most widely used. They can be prepared using Sigma liposome mixtures by concentrating an organic solvent solution of lipids to a thin film on the walls of a container such as by rotary evaporation or by drying in glass with a stream of nitrogen gas. Then, under appropriate conditions, an aqueous solution is added to the thin film and with agitation multilamellar vesicles should form spontaneously with little effort. Large or small unilamellar structured liposomes can also be prepared by varying procedure or conditions. Please see the product insert for Product No. L4031 (the link below) for a step by step protocol that will work for this product also.

  5. How do I prepare the Product L4395, Liposome Kit liposomes?

    Refer to the product information sheet (under Documents, above) for L4031 for preparation information.

  6. I'm thinking of purchasing Product L4395, Liposome Kit.  Why would one use positive vs. negative liposomes?

    Analogous to poly-L-lysine, one may need positively charged agents to deliver drugs to cells, hence positive liposomes.

  7. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  8. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  9. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Abstracts of the Congress of the European Association of Nuclear Medicine. 25-29 August 2001, Napoli, Italy.
European journal of nuclear medicine, 28(8), 961-1298 (2001-09-06)
Evaluation of intracellular and extracellular trehalose as a cryoprotectant of stem cells obtained from umbilical cord blood
Motta JPR, et al.
Cryobiology, 68(3), 343-348 (2014)
Inhibition of cationic liposomes of [3H]thymidine incorporation into DNA of L1210 cells.
Laurent G, Laduron C, Ruysschaert JM, et al.
Research Communications in Molecular Pathology and Pharmacology, 31, 515-527 (1981)
Abd Kakhar Umar et al.
Molecules (Basel, Switzerland), 26(17) (2021-09-11)
Human epidermal growth factor (hEGF) has been known to have excellent wound-healing activity. However, direct application to the wound area can lead to low hEGF bioavailability due to protease enzymes or endocytosis. The use of liposomes as coatings and carriers
S Palakurthi et al.
International journal of pharmaceutics, 290(1-2), 55-62 (2005-01-25)
Conventional lipid microspheres (LM) were prepared using soybean oil and lipid at a 5.5:1 weight ratio with lipid phase consisting of PC (phosphatidyl choline):CH (cholesterol) (1:0.5) by molar ratio. The average diameter of the particles was 150 nm. Long-circulating microspheres

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