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Merck
CN

L0288

Sigma-Aldrich

脂质混合物1,化学成分明确

liquid, sterile-filtered, BioReagent, suitable for cell culture

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别名:
ACF lipid mixture
UNSPSC代码:
12352211
NACRES:
NA.75

生物来源

non-animal source

质量水平

无菌性

sterile-filtered

产品线

BioReagent

形式

liquid

技术

cell culture | mammalian: suitable

杂质

≤10 EU/mL endotoxin

储存温度

2-8°C

一般描述

建议在细胞培养中使用化学成分确定的脂质混合物 1,每升培养基 1 至 10 mL。

应用

脂质混合液1(化学成分确定)已用于:

  • 作为含牛血清白蛋白的Bracket-Oliphant(BO)培养基组分,重悬精子沉淀
  • 作为球状体培养基补充剂,培养原代人皮下基质血管片段细胞
  • 制备脂肪变性细胞模型,用于培养小鼠和人肝细胞

其他说明

含有非动物源性脂肪酸(2 μg/ml花生四烯酸,以及浓度均为10 μg/ml的亚油酸、亚麻酸、肉豆蔻酸、油酸、棕榈酸和硬脂酸)、来自新西兰羊毛的0.22 mg/ml胆固醇、2.2 mg/ml吐温80、70 μg/ml生育酚乙酸酯和100 mg/ml Pluronic F-68,溶剂为细胞培养用水。

相关产品

产品编号
说明
价格

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable


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Anna Ioannidou et al.
The Journal of physiology (2021-08-14)
Adipocyte enlargement is a key feature of obesity and associated with insulin resistance and metabolic disease The cause and consequences of adipocyte enlargement have remained hard to study in vitro due to a lack of human cell models with representative
Juan Zhong et al.
International immunopharmacology, 59, 197-208 (2018-04-16)
The anti-inflammatory, immunomodulatory, and anticancer effects of micheliolide (MCL) isolated from Michelia champaca were previously reported, but its role and underlying mechanisms in relieving liver steatosis remain unclear. Herein, we investigated the effects of MCL on hepatic steatosis using a
Marc P Hübner et al.
PLoS neglected tropical diseases, 14(7), e0008427-e0008427 (2020-07-07)
A major impediment to eliminate lymphatic filariasis and onchocerciasis is the lack of effective short-course macrofilaricidal drugs or regimens that are proven to be safe for both infections. In this study we tested oxfendazole, an anthelmintic shown to be well
Aranya Bagchi et al.
The Journal of experimental biology, 221(Pt 13) (2018-05-12)
Weddell and elephant seals are deep-diving mammals, which rely on lung collapse to limit nitrogen absorption and prevent decompression injury. Repeated collapse and re-expansion exposes the lungs to multiple stressors, including ischemia-reperfusion, alveolar shear stress and inflammation. There is no
Md Mazharul Islam et al.
Reproductive medicine and biology, 20(3), 289-298 (2021-07-16)
The present study was undertaken to clarify whether bovine sperm could take up fatty acids (FAs) and produce ATP to maintain linear motility. Frozen bovine semen was thawed in media containing either lipid mixture (LM) or FAs, and sperm motility

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Importance and uses of linolenic acid in serum-free eukaryotic, including hybridoma and Chinese Hamster Ovary (CHO) cell, cultures

How palmitic acid, other fatty acids and other cell culture components affect the performance of serum-free, protein-free cell culture systems used for biomanufacturing heterologous proteins including monoclonal antibodies.

棕榈酸等脂肪酸和其他细胞培养成分如何影响无血清、无蛋白细胞培养系统(用于生物制造包括单克隆抗体在内的异源蛋白)的性能。

Importance and uses of linoleic acid in serum-free eukaryotic, including hybridoma and Chinese Hamster Ovary (CHO) cell, cultures

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