L2897
LB 琼脂
Highly-referenced microbial growth powder medium with Agar, low salt, suitable for salt-sensitive E. coli culture.
别名:
Lennox broth, Luria Bertani Agar
产品名称
LB 琼脂, Highly-referenced microbial growth powder medium with Agar, low salt, suitable for salt-sensitive E. coli culture.
grade
Molecular Biology
sterility
non-sterile
form
powder
composition
Agar, 15 g/L
NaCl, 5 g/L
Tryptone, 10 g/L
Yeast Extract, 5 g/L
technique(s)
microbiological culture: suitable
application(s)
food and beverages
microbiology
storage temp.
room temp
suitability
nonselective for Escherichia coli
nonselective for coliforms
Quality Level
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Application
- the transformation of competent E. coli cells
- to cultivate Ecc-15 bacteria
- embedding scaffold containing tissue derived from induced pluripotent stem cells (iPSCs)
- transmission electron microscopy (TEM) and immunohistochemistry (IHC)
Features and Benefits
- A budget-friendly alternative to pre-poured plates
- Easy scale-up using larger package sizes
- Standard formulation
General description
Preparation Note
2. Heat to boiling while stirring to dissolve all ingredients completely.
3. Autoclave for 15 minutes at 121°C.
To prepare Lennox L Agar: Add 1 g glucose and proceed with preparation instructions as above.To prepare the medium of Enquist and Sternberg: Aseptically add 10 ml sterile 1 M magnesium sulfate after autoclaving.
Legal Information
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
How do I find price and availability?
There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 or view local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
What are the differences among the Luria, Lennox and Miller LB formulations?
LB, (originally termed lysogeny broth) was initially composed of tryptone, yeast extract, NaCl and glucose. Soon after, the glucose was omitted (Miller's version), and later the NaCl content lowered by half (Lennox's version). For some applications, even lower salt is required (Luria's low salt version).
What is the difference among the many LB - Lennox products?
LB - Lennox is available in many types to suit your needs. The different product formats include powder, EZMix™ powder, and tablet form. Each of these forms are available with and without agar for easy LB-agar plate preparation.L3022 (LB Lennox powder)L2897 (LB Lennox powder with agar)L7275 (LB Lennox tablets)L7025 (LB Lennox tablets with agar)L7658 (LB Lennox EZMix(TM) powder)L7533 (LB Lennox EZMix(TM) powder with agar)L5542 (LB Lennox pre-poured agar plates)
Which bacterial culture medium is the best choice for my application?
Each of the broths will likely grow E. coli very well, but there are still general guidelines for choosing a broth when you are working without a protocol. Generally:LB - Miller and LB - Lennox are used for E. coli growth and maintanence, DNA plasmid production and protein production. The Lennox formulation has a lower salt content required for some salt-sensitive selection antibiotics.LB - Luria low salt is used for special applications where the E. coli growth or other constraints require the lowest possible salt content.Terrific Broth is used for higher yield protein production and high yield DNA plasmid production, because of the faster growth of the E. coli in this medium.SOB is used for protein production, DNA plasmid production and the generation of high-efficiency competent cells.SOC is used for initial growth of competent cells and the transformation procedure.
Will adding magnesium to the culture medium increase cell density?
In microbial broth formulations that do not already contain magnesium, the addition of 10-20 mM MgCl2 or MgSO4 may increase cell densities. You may need to also increase the shaking speed of the incubator.
My question is not addressed here, how can I contact Technical Service for assistance?
Ask a Scientist here.
实验方案
Preparation of Plasmid DNA by Alkaline Lysis with SDS: Maxipreparation between Cold Spring Harbor Laboratory Press and our research team.
General protocols for growth of competent cells in microbial medium.
在从小型培养到发酵级的不同规模下培养大肠杆菌。包括了用于启动起始培养物、悬浮细胞、单一培养物和平板噬菌体M13的实验方案。
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