所有图片(3)
About This Item
经验公式（希尔记法）:
C9H18O5S
CAS号:
分子量:
238.30
Beilstein:
4631
MDL编号:
UNSPSC代码:
12352200
PubChem化学物质编号:
NACRES:
NA.52
推荐产品
等级
for molecular biology
质量水平
描述
non-ionic
表单
liquid
分子量
238.3
运输
dry ice
储存温度
−20°C
SMILES字符串
CC(C)S[C@@H]1O[C@H](CO)[C@H](O)[C@H](O)[C@H]1O
InChI
1S/C9H18O5S/c1-4(2)15-9-8(13)7(12)6(11)5(3-10)14-9/h4-13H,3H2,1-2H3/t5-,6+,7+,8-,9+/m1/s1
InChI key
BPHPUYQFMNQIOC-NXRLNHOXSA-N
一般描述
Isopropyl thiogalactose (IPTG) is a sugar derivative widely used for the induction of the expression of recombinant proteins in E. coli. This expression system requires that the gene of interest is cloned in plasmids containing the Lac promoter. IPTG Ready Made is a 0.2 μm filter sterilized solution of 200 mM IPTG. This reagent remains liquid at its storage temperature (-20 °C), thus avoiding freeze-thaw cycles and providing a user-friendly solution.
应用
Isopropyl β-D-thiogalactopyranoside solution is:
- used as an inducer for the expression of single domain antibodies(sdAbs) in E coli and bioactive peptides
- used in its half-life and decay studies
- suitable for induction of recombinant protein expression in systems controlled by the lac repressor
生化/生理作用
Isopropyl β-D-thiogalactopyranoside binds to the lac repressor and releases the tetrameric repressor from the lac operator in an allosteric manner. This binding allows the transcription of genes in the lac operon, such as the gene coding for β--galactosidase. The β-galactosidase gene is transcribed and monosaccharides are the product. Since Isopropyl β-D-thiogalactopyranoside cannot be metabolized by E. coli its concentration remains constant. Therefore, it is not a variable in the experiment.
原理
Isopropyl β-D-thiogalactopyranoside binds to the lac repressor and releases the tetrameric repressor from the lac operator in an allosteric manner. This binding allows the transcription of genes in the lac operon, such as the gene coding for β-galactosidase. The β-galactosidase gene is transcribed and monosaccharides are the product.
相关产品
产品编号
说明
价格
储存分类代码
10 - Combustible liquids
WGK
WGK 2
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
从最新的版本中选择一种：
分析证书(COA)
Lot/Batch Number
Engineering of ultra-small diagnostic nanoprobes through oriented conjugation of single-domain antibodies and quantum dots
Sukhanova A, et al.
Protocol Exchange, 10 (2012)
Random sequences are an abundant source of bioactive RNAs or peptides
Neme R, et al.
Nature ecology & evolution, 1(6), 0127-0127 (2017)
Half-life measurements of chemical inducers for recombinant gene expression
Pasotti L, et al.
Journal of Biological Engineering, 8(1), 5-5 (2014)
L H Hansen et al.
Current microbiology, 36(6), 341-347 (1998-06-03)
The role of the Escherichia coli lacY gene product (the lactose permease) in the induction of isopropyl-beta-D-thiogalactopyranoside (IPTG) inducible promoters was studied in E. coli and P. fluorescens. This was done by comparing strains containing a lacIPOZYA chromosomal insert with
Iltaf Ahmed et al.
Human molecular genetics, 24(11), 3172-3180 (2015-02-24)
There are two known mRNA degradation pathways, 3' to 5' and 5' to 3'. We identified likely pathogenic variants in two genes involved in these two pathways in individuals with intellectual disability. In a large family with multiple branches, we
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系技术服务部门