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HSRT100

Sigma-Aldrich

增强型禽类HS RT-PCR试剂盒

Flexible kit for one-step or two-step RT-PCR

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¥8,594.96

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1 KIT
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About This Item

UNSPSC代码:
41106303
NACRES:
NA.55

¥8,594.96

请联系客服了解存货情况
新价格，新优惠！

质量水平

200

用途

sufficient for 100 reactions

特点

dNTPs included
hotstart

技术

RT-PCR: suitable

颜色

colorless

输入

purified RNA

运输

wet ice

储存温度

−20°C

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此商品
STR1QR0100TOSRTRO
增强型禽类HS RT-PCR试剂盒 Flexible kit for one-step or two-step RT-PCR

HSRT100

增强型禽类HS RT-PCR试剂盒

增强型禽类第一链合成试剂盒 Components for cDNA synthesis with enhanced AMV reverse transcriptase

STR1

增强型禽类第一链合成试剂盒

SYBR® Green 定量 RT-qPCR 试剂盒 One step SYBR® Green RT-qPCR with MMLV & hot-start Taq DNA Polymerase

QR0100

SYBR® Green 定量 RT-qPCR 试剂盒

Transcriptor 一步法 RT-PCR 试剂盒 sufficient for 50 reactions, sufficient for 150 reactions, suitable for RT-qPCR, suitable for RT-PCR, hotstart

TOSRTRO

Transcriptor 一步法 RT-PCR 试剂盒

usage

sufficient for 100 reactions

usage

sufficient for 50 reactions,  kit sufficient for 50 reactions

usage

sufficient for 250 reactions

usage

sufficient for 150 reactions, sufficient for 50 reactions

feature

dNTPs included

feature

dNTPs included, hotstart: no

feature

dNTPs included, hotstart

feature

hotstart

color

colorless

color

colorless

color

colorless

color

-

input

purified RNA

input

purified RNA

input

purified RNA

input

purified RNA

shipped in

wet ice

shipped in

dry ice

shipped in

wet ice

shipped in

-

一般描述

提供1步法和2步法RT-PCR反应。

1步法： 同一管中的eAMV™ RT逆转录酶和AccuTaq LA DNA聚合酶相继发挥作用，首先生成cDNA，紧接着进行PCR扩增反应。 用于灵敏、快速的RNA分析。

2步法： 每次反应都经过单独优化，可在高保真前提下获得更高产率。适合需要更多扩增的实验，或高产率比便利性更加重要的实验。

应用

增强型禽类HS RT-PCR试剂盒用于合成反转录酶PCR (RT-PCR) 分析的第一条cDNA链。[1][2][3][4][5]

特点和优势

  • 该反转录酶可制备更长的转录本，生成长达14.1kb的cDNA。
  • 低丰度mRNA检测灵敏度更高。eAMV™ RT能够成功转录其他反转录酶无法检测到的微量RNA。
  • 针对复杂的二级结构的RNA,拥有超强的转录能力。
  • JumpStart AccuTaq LA DNA聚合酶有利于提高灵敏度、特异性与得率。

其他说明

Reverse Transcriptase PCR (RT-PCR)是一种强大的基因表达研究工具。Enhanced Avian HS RT-PCR试剂盒应用了一款加强型禽成髓细胞瘤病毒逆转录酶（eAMV-RT），相比标准型AMV-RT和MMLV-RT具有更佳的反应性能。eAMV RT是一种效率极强的酶，在较高温度下（65 °C）仍能够打开复杂RNA的二级结构成功介导转录反应，是以总RNA或poly（A）+ RNA为模板制备高质量全长cDNA（长达14.1kb）的最佳酶蛋白。同时本品也附赠JumpStartTM ™AccuTaq™ LA DNA聚合酶混合物，可减少非特异性扩增，增加反应特异性与灵敏度。这两种酶的组合为用户提供了一个高品质的反应系统，能够同时兼容一步法或两步法的RT-PCR方案。

法律信息

No license is conveyed with the purchase of this product under any of US Patents Nos. 5,804,375, 5,994,056, 6,171,785, 6,214,979, 5,538,848, 5,723,591, 5,876,930, 6,030,787, and 6,258,569, and corresponding patents outside the United States, or any other patents or patent applications, relating to the 5′ Nuclease and dsDNA-Binding Dye Processes. For further information contact the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
AccuTaq is a trademark of Sigma-Aldrich Co. LLC
JumpStart is a trademark of Sigma-Aldrich Co. LLC
eAMV is a trademark of Sigma-Aldrich Co. LLC

试剂盒组分也可单独购买

产品编号
说明
化学品安全说明书
  • O4387Random nonamers 100 μL
  • O4387Anchored oligo(dT)23 primers 100 μL
  • B017410x reaction buffers 10x AccuTaq buffer
  • P219210 mM dNTP mix 10 x PCR buffer
  • W1754Nuclease-free water 4 x 1.5

危险声明

H412

预防措施声明

P273 - P501

危险分类

Aquatic Chronic 3

储存分类代码

10 - Combustible liquids

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

常规特殊物品
含少量动物源组分生物产品

    • 历史批次信息供参考:

    分析证书(COA)

    Lot/Batch Number
    SLCJ9486
    SLCH1117
    SLCG5943
    SLCG5940
    SLCD4474

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    商品

    Reverse Transcription

    Challenges in gene expression analysis include mRNA stability, temporal transcription patterns, and mRNA-protein correlation, impacting accuracy.

    逆转录

    分析基因表达的方法之一是测量基因的mRNA浓度。此类分析面临着若干挑战，例如不同转录本之间的半衰期不同、转录时间模式以及mRNA和蛋白质之间缺乏相关性。

    实验方案

    The 3'/5' Assay for Analysis of RNA Integrity Protocol

    The 3’/5’ integrity assay identifies RNA degradation, crucial for large sample sets or less detectable degradation.

    Long & Accurate PCR with RedAccuTaq®

    REDAccuTaq LA protocol offers high-fidelity amplification of long PCR fragments with direct gel loading capability.

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