产品名称
Anti-Heat Shock Protein 27/25 antibody produced in rabbit, affinity isolated antibody, buffered aqueous solution
biological source
rabbit
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen 25 kDa (mouse)
antigen 27 kDa (human)
species reactivity
human, canine, rat, mouse
technique(s)
indirect immunofluorescence: 4-8 μg/mL using MDCK cell line.
microarray: suitable
western blot: 0.5-1 μg/mL using whole extract of rat fibroblast Rat-1 cell line or Madin-Darby canine kidney (MDCK) cell line.
western blot: 1-2 μg/mL using mouse brain extract (S1 fraction) or human HeLa cell line
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... HSPB1(3315)
mouse ... Hspb1(15507)
rat ... Hspb1(24471)
Application
Anti-Heat Shock Protein 27/25 antibody produced in rabbit may be used in western blotting and immunofluorescence.
Biochem/physiol Actions
Anti-Heat Shock Protein 27/25 (HSP27/25) recognizes HSP27 (27 kDa) (mouse HSP25).
Heat shock protein 27 (HSP27) accumulates in cells exposed to a short period of hyperthermia and contributes to the development of a transient state of thermotolerance. This protein is stimulated by various cytokines, growth factors, hormones, and chemicals. HSP27 shows rapid phosphorylation following exposure to stress stimuli. HSP27 acts as an actin-cap binding protein and can inhibit actin polymerization, thus modulating actin dynamics during stress. It also protects against apoptotic cell death triggered by a variety of stimuli including hyperthermia, oxidative stress, Fas ligand, and cytotoxic drugs. HSP27 interferes specifically with the mitochondrial pathway of caspase-induced cell death by acting as a negative regulator of cytochrome c-dependent activation of caspase-3.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
Heat shock protein 27 (HSP27), also known as mouse HSP25, is a highly conserved oligomeric protein. This protein is expressed constitutively in many cell types and tissues, such as the placenta, breast, skin, platelets, uterus and cervix. HSP27 is expressed in the cytoplasm and localizes to the nucleus upon stress stimuli. The HSPB1 or the HSP27 gene is mapped on the human chromosome at 7q11.23.
Immunogen
synthetic peptide corresponding to amino acids 190-209 located at the C-terminus of mouse HSP27/25, conjugated to KLH. The sequence is identical in dog and Chinese hamster, highly conserved in rat HSP27 (85% identity), and to a lesser extent in human HSP27 (65%).
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Preparation Note
For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. Storage in frost-free freezers is also not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilu-tions should be discarded if not used within 12 hours.
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存储类别
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
法规信息
常规特殊物品
此项目有
P Mehlen et al.
The Journal of biological chemistry, 272(50), 31657-31665 (1998-02-12)
Small stress proteins are developmentally regulated and linked to cell growth and differentiation. The early phase of murine embryonic stem (ES) cell differentiation, characterized by a gradual growth arrest, is accompanied with hsp27 transient accumulation. This differentiation process also correlated
D R Ciocca et al.
Journal of the National Cancer Institute, 85(19), 1558-1570 (1993-10-06)
Heat shock and other environmental and pathophysiologic stresses stimulate synthesis of heat shock proteins (Hsps). These proteins enable the cell to survive and recover from stressful conditions by as yet uncompletely understood mechanisms. Hsp27 is an important small Hsp (molecular
P Pandey et al.
Oncogene, 19(16), 1975-1981 (2000-05-10)
The release of mitochondrial cytochrome c by genotoxic stress induces the formation of a cytosolic complex with Apaf-1 (mammalian CED4 homolog) and thereby the activation of procaspase-3 (cas-3) and procaspase-9 (cas-9). Here we demonstrate that heat-shock protein 27 (Hsp27) inhibits
Pragya Verma et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 34(5), 1599-1612 (2014-01-31)
Heat shock factor 1 (HSF1) protects neurons from death caused by the accumulation of misfolded proteins. It is believed that this protective effect is mediated by the transcriptional stimulation of genes encoding heat shock proteins (HSPs), a family of chaperones
Constantin d'Ydewalle et al.
Nature medicine, 17(8), 968-974 (2011-07-26)
Charcot-Marie-Tooth disease (CMT) is the most common inherited disorder of the peripheral nervous system. Mutations in the 27-kDa small heat-shock protein gene (HSPB1) cause axonal CMT or distal hereditary motor neuropathy (distal HMN). We developed and characterized transgenic mice expressing
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