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安全信息

H2289

Sigma-Aldrich

Anti-Heat Shock Protein 27/25 antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

别名:

Anti-HSP27/25

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About This Item

UNSPSC代码:
12352203
NACRES:
NA.41

生物来源

rabbit

偶联物

unconjugated

抗体形式

affinity isolated antibody

抗体产品类型

primary antibodies

克隆

polyclonal

表单

buffered aqueous solution

分子量

antigen 25 kDa (mouse)
antigen 27 kDa (human)

种属反应性

human, canine, rat, mouse

技术

indirect immunofluorescence: 4-8 μg/mL using MDCK cell line.
microarray: suitable
western blot: 0.5-1 μg/mL using whole extract of rat fibroblast Rat-1 cell line or Madin-Darby canine kidney (MDCK) cell line.
western blot: 1-2 μg/mL using mouse brain extract (S1 fraction) or human HeLa cell line

UniProt登记号

P04792

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P1498H0273H7287
Anti-Heat Shock Protein 27/25 antibody produced in rabbit affinity isolated antibody, buffered aqueous solution

H2289

Anti-Heat Shock Protein 27/25 antibody produced in rabbit

Anti-Heat Shock Protein 27 antibody produced in rabbit IgG fraction of antiserum, buffered aqueous solution

P1498

Anti-Heat Shock Protein 27 antibody produced in rabbit

Monoclonal Anti-Heat Shock Protein 25 antibody produced in mouse clone IAP-28, ascites fluid

H0273

Monoclonal Anti-Heat Shock Protein 25 antibody produced in mouse

Anti-Heat Shock Protein 110 (HD-19) antibody produced in rabbit affinity isolated antibody, buffered aqueous solution

H7287

Anti-Heat Shock Protein 110 (HD-19) antibody produced in rabbit

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody form

IgG fraction of antiserum

antibody form

ascites fluid

antibody form

affinity isolated antibody

biological source

rabbit

biological source

rabbit

biological source

mouse

biological source

rabbit

technique(s)

indirect immunofluorescence: 4-8 μg/mL using MDCK cell line., western blot: 0.5-1 μg/mL using whole extract of rat fibroblast Rat-1 cell line or Madin-Darby canine kidney (MDCK) cell line., microarray: suitable, western blot: 1-2 μg/mL using mouse brain extract (S1 fraction) or human HeLa cell line

technique(s)

indirect immunofluorescence: 1:250-1:500 using HeLa cells, microarray: suitable, western blot: 1:4,000-1:8,000 using whole cell extract of human epitheloid carcinoma HeLa cell line or human epidermoid carcinoma A431 cell line

technique(s)

immunohistochemistry: suitable using frozen sections of chicken gizzard smooth muscle, indirect ELISA: suitable, indirect immunofluorescence: suitable using formalin-fixed, heat-shocked cultured chick embryo fibroblasts, radioimmunoassay: suitable, western blot: 1:500 using turkey gizzard extract

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 2-4 μg/mL using rat cerebellum, microarray: suitable, western blot: 0.5-1 μg/mL using cytosolic fraction of rat brain and whole cell extract of mouse fibroblast NIH3T3 cell line

clone

polyclonal

clone

polyclonal

clone

IAP-28, monoclonal

clone

polyclonal

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

一般描述

Heat shock protein 27 (HSP27), also known as mouse HSP25, is a highly conserved oligomeric protein. This protein is expressed constitutively in many cell types and tissues, such as the placenta, breast, skin, platelets, uterus and cervix. HSP27 is expressed in the cytoplasm and localizes to the nucleus upon stress stimuli. The HSPB1 or the HSP27 gene is mapped on the human chromosome at 7q11.23.

特异性

Anti-Heat Shock Protein 27/25 (HSP27/25) recognizes HSP27 (27 kDa) (mouse HSP25).

免疫原

synthetic peptide corresponding to amino acids 190-209 located at the C-terminus of mouse HSP27/25, conjugated to KLH. The sequence is identical in dog and Chinese hamster, highly conserved in rat HSP27 (85% identity), and to a lesser extent in human HSP27 (65%).

应用

Anti-Heat Shock Protein 27/25 antibody produced in rabbit may be used in western blotting[1][2] and immunofluorescence.

生化/生理作用

Heat shock protein 27 (HSP27) accumulates in cells exposed to a short period of hyperthermia and contributes to the development of a transient state of thermotolerance. This protein is stimulated by various cytokines, growth factors, hormones, and chemicals. HSP27 shows rapid phosphorylation following exposure to stress stimuli. HSP27 acts as an actin-cap binding protein and can inhibit actin polymerization, thus modulating actin dynamics during stress. It also protects against apoptotic cell death triggered by a variety of stimuli including hyperthermia, oxidative stress, Fas ligand, and cytotoxic drugs. HSP27 interferes specifically with the mitochondrial pathway of caspase-induced cell death by acting as a negative regulator of cytochrome c-dependent activation of caspase-3.

外形

0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。

储存及稳定性

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. Storage in frost-free freezers is also not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilu-tions should be discarded if not used within 12 hours.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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储存分类代码

10 - Combustible liquids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

法规信息

常规特殊物品

    • 历史批次信息供参考:

    分析证书(COA)

    Lot/Batch Number
    075K4806

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    D R Ciocca et al.
    Journal of the National Cancer Institute, 85(19), 1558-1570 (1993-10-06)
    Heat shock and other environmental and pathophysiologic stresses stimulate synthesis of heat shock proteins (Hsps). These proteins enable the cell to survive and recover from stressful conditions by as yet uncompletely understood mechanisms. Hsp27 is an important small Hsp (molecular
    P Mehlen et al.
    The Journal of biological chemistry, 272(50), 31657-31665 (1998-02-12)
    Small stress proteins are developmentally regulated and linked to cell growth and differentiation. The early phase of murine embryonic stem (ES) cell differentiation, characterized by a gradual growth arrest, is accompanied with hsp27 transient accumulation. This differentiation process also correlated
    P Pandey et al.
    Oncogene, 19(16), 1975-1981 (2000-05-10)
    The release of mitochondrial cytochrome c by genotoxic stress induces the formation of a cytosolic complex with Apaf-1 (mammalian CED4 homolog) and thereby the activation of procaspase-3 (cas-3) and procaspase-9 (cas-9). Here we demonstrate that heat-shock protein 27 (Hsp27) inhibits
    Constantin d'Ydewalle et al.
    Nature medicine, 17(8), 968-974 (2011-07-26)
    Charcot-Marie-Tooth disease (CMT) is the most common inherited disorder of the peripheral nervous system. Mutations in the 27-kDa small heat-shock protein gene (HSPB1) cause axonal CMT or distal hereditary motor neuropathy (distal HMN). We developed and characterized transgenic mice expressing
    Pragya Verma et al.
    The Journal of neuroscience : the official journal of the Society for Neuroscience, 34(5), 1599-1612 (2014-01-31)
    Heat shock factor 1 (HSF1) protects neurons from death caused by the accumulation of misfolded proteins. It is believed that this protective effect is mediated by the transcriptional stimulation of genes encoding heat shock proteins (HSPs), a family of chaperones
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