跳转至内容

尊敬的客户:

目前国际形势复杂多变,关税政策尚不明朗,这可能对我们的产品价格产生一定影响。在此情况下,我们希望就订单事宜与您进行友好沟通。

基于当前的不确定性,如果您选择在此期间下单,我们将保留根据实际情况调整价格的权利。同时,我们也理解市场变化可能给您带来的困扰,因此如果在订单实际发货前因关税政策变动导致价格出现较大波动,默克将与您进行协商讨论并视情况对订单进行调整或取消。

Merck
CN
所有图片(1)

主要文件

安全信息

GERPN225

cAMP, Biotrak EIA System

Cytiva RPN225

登录查看公司和协议定价


About This Item

UNSPSC代码:
41116126
NACRES:
NA.32

制造商/商品名称

Cytiva RPN225

包装

pkg of 96 wells

储存温度

2-8°C

一般描述

EIAs and ELISAs are convenient, high-sensitivity, peroxidase-based technologies that enable the amount of protein present in a sample to be measured in a single day. Novel lysis reagents have been developed (Lysis Reagents 1 and 2) to facilitate simple and rapid extraction of cellular molecules.

The Biotrak cAMP competitive enzyme immunoassay system is specifically designed for research purposes. The kit includes protocols using novel lysis reagents in order to facilitate simple and rapid extraction of cAMP from cell cultures. These components avoid the requirement for traditional, time-consuming extraction procedures and eliminates the need for removal of extraction reagents prior to measurement. It combines the use of a peroxidase-labeled cAMP conjugate, a specific antiserum which can be immobilized on to precoated microplates, and a one-pot stabilized substrate solution.

Lysis reagent 1 hydrolyzes cell membranes to release intra-cellular
cAMP. Lysis Reagent 2 sequesters the key component in Lysis
Reagent 1 and ensures cAMP is free for subsequent analysis. The
detergent/sequestrant complex does not interfere with antigen:
antibody binding. Lysis Reagent 1 is simply added to cultured cells,
followed by a 5 to 10 min incubation before assay. The
antiserum is reconstituted with Lysis Reagent 2. The assay is based
on competition between unlabeled cAMP and a fixed quantity of
peroxidase-labeled cAMP, for a limited number of binding sites on a
cAMP specific antibody.

特点和优势

  • Elimination of inconvenient, time-consuming extraction procedures.
  • Flexible method-choice of assay protocols.
  • Dual range 25 - 6400 fmol/well (non-acetylation protocol), 2–128 fmol/well (acetylation protocol).
  • Rapid assay protocol.
  • Non-radioactive
  • Specific for cAMP.
  • Precise and accurate measurement
  • Ready-to-use substrate.
  • Colour coded reagents

分析说明

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

法律信息

Biotrak is a trademark of Cytiva

象形图

Corrosion

警示用语:

Danger

储存分类代码

13 - Non Combustible Solids

法规信息

新产品
  • 技术规格说明书

  • 历史批次信息供参考:

    分析证书(COA)

    Lot/Batch Number

    It looks like we've run into a problem, but you can still download Certificates of Analysis from our 文件 section.

    如需帮助,请联系 客户支持

    已有该产品?

    在文件库中查找您最近购买产品的文档。

    访问文档库

    我们的科学家团队拥有各种研究领域经验,包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

    联系客户支持