制造商/商品名称
Cytiva RPN2251
包装
pkg of 96 wells
储存温度
2-8°C
一般描述
EIAs and ELISAs are convenient, high-sensitivity, peroxidase-based technologies that enable the amount of protein present in a sample to be measured in a single day. Novel lysis reagents have been developed (Lysis Reagents 1 and 2) to facilitate simple and rapid extraction of cellular molecules.
The Biotrak™ cAMP competitive enzymeimmunoassay system is specifically designed for research purposes. The kit includes protocols using novel lysis reagents in order to facilitate simple and rapid extraction of cAMP from cell cultures. These components avoid the requirement for traditional, time-consuming extraction procedures and obviate the need for removal of extraction reagents prior to measurement. It combines the use of a peroxidase-labeled cAMP conjugate, a specific antiserum which can be immobilized on to precoated microplates, and a one-pot stabilized substrate solution.
Lysis reagent 1 hydrolyzes cell membranes to release intra-cellular
cAMP. Lysis Reagent 2 sequesters the key component in Lysis
Reagent 1 and ensures cAMP is free for subsequent analysis. The
detergent/sequestrant complex does not interfere with antigen:
antibody binding. Lysis Reagent 1 is simply added to cultured cells,
followed by a 5 to 10 min incubation before assay. The
antiserum is reconstituted with Lysis Reagent 2. The assay is based
on competition between unlabeled cAMP and a fixed quantity of
peroxidase-labeled cAMP, for a limited number of binding sites on a
cAMP specific antibody.
The Biotrak™ cAMP competitive enzymeimmunoassay system is specifically designed for research purposes. The kit includes protocols using novel lysis reagents in order to facilitate simple and rapid extraction of cAMP from cell cultures. These components avoid the requirement for traditional, time-consuming extraction procedures and obviate the need for removal of extraction reagents prior to measurement. It combines the use of a peroxidase-labeled cAMP conjugate, a specific antiserum which can be immobilized on to precoated microplates, and a one-pot stabilized substrate solution.
Lysis reagent 1 hydrolyzes cell membranes to release intra-cellular
cAMP. Lysis Reagent 2 sequesters the key component in Lysis
Reagent 1 and ensures cAMP is free for subsequent analysis. The
detergent/sequestrant complex does not interfere with antigen:
antibody binding. Lysis Reagent 1 is simply added to cultured cells,
followed by a 5 to 10 min incubation before assay. The
antiserum is reconstituted with Lysis Reagent 2. The assay is based
on competition between unlabeled cAMP and a fixed quantity of
peroxidase-labeled cAMP, for a limited number of binding sites on a
cAMP specific antibody.
应用
cAMP Biotrak™ EIA, (Non-Acetylation Protocol) has been used to measure intracellular cyclic adenosine monophosphate (cAMP) levels.
特点和优势
- Elimination of inconvenient, time-consuming extraction
procedures. - Flexible method-choice of assay protocols.
- Range 25 - 6400 fmol/well (non-acetylation protocol).
- Rapid assay protocol.
- Nonradioactive.
- Specific for cAMP.
- Precise and accurate measurement
- Ready-to-use substrate.
- Colour coded reagents
储存及稳定性
Please be aware this product may be shipped 30 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
分析说明
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
法律信息
Biotrak is a trademark of Cytiva
警示用语：
Warning
法规信息
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