产品名称
Superdex® 200 Increase 10/300 GL, Cytiva 28-9909-44, column L × I.D. 30 cm × 10 mm, 8.6 μm particle size
material
glass column
polyethylene filter
product line
Superdex®
form
prepacked column
crosslinking
cross-linked
mol wt
protein Mr 1.3 MDa (exclusion limit)
protein Mr 10-600 kDa (fractionation range)
Mp 1-100 kDa (dextrans fractionation range)
packaging
pkg of 1 ea
manufacturer/tradename
Cytiva 28-9909-44
storage condition
(20% ethanol)
parameter
0.75 mL/min flow rate
1.8 mL/min max. flow rate
25-500 μL sample volume
3.0 Mpa pressure drop required
technique(s)
liquid chromatography (LC): suitable
bed volume
~24 mL
column L × I.D.
30 cm × 10 mm
matrix
cross-linked agarose-dextran composite
matrix active group
dextran-agarose
particle size
8.6 μm
cleaning
1-14
operating pH
3—12
separation technique
size exclusion (SEC)
shipped in
ambient
storage temp.
2-30°C
相关类别
Application
Features and Benefits
- 增强性能：与前代Superdex™ 200相比，分离度和运行时间有所提高。
- 分离度极高：小珠子尺寸和窄粒度分布提供高分离度，可实现高蛋白质纯度。
- 高流速：硬珠提供出色的压力/流量特性。
General description
Superdex™ 200 Increase 是用于 SEC 的新一代葡聚糖-琼脂糖复合基质。Superdex™ Increase 柱的珠粒比前代产品更小、更坚硬，可在更短的运行时间内提供更高的分离度。
Superdex™ 200 Increase具有优化选择性曲线，可对分子量范围约 100 000 至 300 000 的抗体和大分子实现出色的分离效果。它非常适合检测和分离单克隆抗体制剂中的抗体单体、二聚体和聚集体。这种树脂出色的分离能力有助于鉴定蛋白质，包括分析抗体大小变异体、评估膜蛋白大小均一性以及研究蛋白质-蛋白质相互作用。
Superdex™ 200 Increase 经过优化，可对分子量范围约100 000 至 300 000 的蛋白质实现高分离度分离。Superose™ 6 Increase具有与之互补的分离范围，可分离 Superdex 200 Increase 无法分离的超大蛋白质复合物和大分子。
这种琼脂糖树脂具有耐碱性，并支持就地清洗 (CIP) 程序。此功能允许同一色谱柱用于不同蛋白质，同时最大程度降低样品间交叉污染风险。
Preparation Note
Legal Information
signalword
Warning
hcodes
存储类别
3 - Flammable liquids
商品
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This page shows how to perform a purification and homogeneity check of membrane proteins with products from Cytiva.
Understanding pressure drop in chromatography systems is crucial for optimal functionality across different parts of the system.
实验方案
Superdex from Cytiva are SEC media consisting of a composite base matrix of dextran and agarose. This page shows how to perform a separation with a superdex column.
This page shows how to refold proteins using gel filtration and products from Cytiva.
Ensure chromatographic sample clarity to avoid column clogging, reduce washing needs, and prolong column life for purification.
This page discusses how to do a purification with SOURCE™ media from Cytiva.
相关内容
Superdex® from Cytiva are Size Exclusion Chromatography media consisting of a composite base matrix of dextran and agarose. This page shows how to perform a separation with a Superdex® column.
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