Separation Options with Superdex^®

Superdex^® is produced with different average particle sizes and different selectivities (see Table 2.2).

• Use the prepacked columns with Superdex^® Peptide, Superdex^® 75, and Superdex^® 200 Increase for small-scale preparative purification and analytical runs.
• Use columns prepacked with Superdex^® 30 prep grade, Superdex^® 75 prep grade, or Superdex^® 200 prep grade media for preparative applications.
• Use bulk media of Superdex^® 30 prep grade, Superdex^® 75 prep grade, and Superdex^® 200 prep grade for preparative purification with larger sample volumes of up to 13 mL.
• The maximum pressure drop over the packed bed is a typical value. Note that the actual value is individual for each column and needs to be determined (see Setting column pressure limits, Chapter 1).
• Start with Superdex^® 200 Increase when the molecular weight of the protein of interest is unknown. Superdex^® 200 Increase or Superdex^® 200 prep grade are especially suitable for the separation of monoclonal antibodies from dimers and from contaminants of lower molecular weight, for example albumin and transferrin.
• Start with Superdex^® Peptide or Superdex^® 30 prep grade for separations of peptides, oligonucleotides and small proteins below Mr 10 000.
• Exposure to temperatures outside the range 4 °C to 40 °C will negatively affect the efficiency of the packed bed and the column will need to be repacked.