推荐产品
包装
pack of 5 × 5 mL
制造商/商品名称
Cytiva 17-1408-01
储存条件
dry media
参数
42 psi
柱床尺寸
16 mm × 25 mm
柱床体积
5 mL
色谱柱内径
16 mm
基质
cross-linked dextran
粒径
>20 μm (dry)
cleaning
2-13
工作范围
2-13
适用性
suitable for bioprocess medium
一般描述
HiTrap® Desalting is a prepacked, ready to use column for group separation between high and low molecular weight substances, i.e., buffer exchange prior to or after different chromatographic steps, removal of low molecular weight contaminants or removal of reagents to terminate a reaction.
应用
特点和优势
- Handles sample volumes of 1.5 mL, or larger volumes by connecting up to five columns in series.
- Replaces dialysis as the method of choice for desalting.
- Desalting requires less than one minute due to the high rigidity of Sephadex® G-25 and the optimized packing technology.
储存及稳定性
分析说明
其他说明
法律信息
警示用语：
Warning
危险声明
储存分类代码
3 - Flammable liquids
商品
This page shows how to perform a purification and on-column refolding of an insoluble his-tagged protein from an E. coli culture with HisTrap™ FF columns and ÄKTAprime from Cytiva.
GST-tagged protein yield varies based on protein, host cell, and culture conditions, influencing production.
This page shows how to perform a purification of His-tagged membrane proteins.
This page covers detailed aspects of each step in an IEX separation to improve resolution and overall performance.
实验方案
This page shows how to condition membrane proteins for further analysis with products from Cytiva.
Rules and good practice in sample preparation for Western blot sample preparation from cell culture and tissue samples.
How to perform buffer exchange and desalting with Sephadex G-25, HiTrap Desalting columns, or ÄKTAprime plus.
如何使用Sephadex G-25、HiTrap脱盐柱或ÄKTAprime plus进行缓冲液置换和脱盐。
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