一般描述
Phenyl Sepharose™ 6 Fast FLow is a well established, standard aromatic hydrophobic interaction chromatography (HIC) medium for capture and intermediate purification requiring low to medium hydrophobicity.
应用
Phenyl Sepharose™ 6 Fast FLow is part of the Sepharose™ Fast FLow HIC platform, which has been an industrial standard for HIC chromatography during recent decades.
Phenyl Sepharose™ 6 Fast FLow is composed of cross-linked 6% Agarose beads modified with standard aromatic phenyl groups via uncharged, chemically-stable ether linkages. Two levels of ligand substitution degree help to find the optimal selectivity and binding capacity for a given application.
Phenyl Sepharose™ 6 Fast FLow is available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development. As member of the BioProcess media range, Phenyl Sepharose™ 6 Fast FLow meets industrial demands with security of supply and comprehensive technical and regulatory support.
Phenyl Sepharose™ 6 Fast FLow is composed of cross-linked 6% Agarose beads modified with standard aromatic phenyl groups via uncharged, chemically-stable ether linkages. Two levels of ligand substitution degree help to find the optimal selectivity and binding capacity for a given application.
Phenyl Sepharose™ 6 Fast FLow is available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development. As member of the BioProcess media range, Phenyl Sepharose™ 6 Fast FLow meets industrial demands with security of supply and comprehensive technical and regulatory support.
特点和优势
- Standard aromatic HIC media based on Sepharose™ Fast FLow base matrix derivatized via uncharged, chemically-stable ether linkages.
- Designed for initial and intermediate purification requiring Low to medium hydrophobicity
- Two levels of substitution help find optimal selectivity and binding capacity for a given application
- BioProcess medium supported for industrial applications and well-established in approved processes
- The hydrophilic nature of the base matrix ensures Low levels of non-specific binding.
储存及稳定性
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)
分析说明
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
法律信息
Sepharose is a trademark of Cytiva
警示用语：
Warning
危险声明
储存分类代码
3 - Flammable liquids
法规信息
新产品
商品
Optimize salt conditions for protein binding and impurity passage in chromatography to achieve required selectivity.
Column, media, and sample preparation for Hydrophobic Interaction Chromatography (HIC) detailed with Cytiva media.
实验方案
Selectivity of HIC medium from Cytiva is based on properties such as hydrophobicity, porosity and particle size.
Here we discuss purification options when working with Sepharose® High Performance and we present some examples of media screening, capture and purification.
This page discusses various aspects of sample preparation for chromatographic purification.
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