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Merck
CN
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主要文件

安全信息

F3165

Sigma-Aldrich

单克隆抗-FLAG® M2 小鼠抗

clone M2, purified immunoglobulin (Purified IgG1 subclass), buffered aqueous solution (10 mM sodium phosphate, 150 mM NaCl, pH 7.4, containing 0.02% sodium azide)

别名:

抗 ddddk, 抗 dykddddk

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500 G
CN¥1,303.68
1 KG
CN¥1,738.93
5 KG
CN¥4,964.53

CN¥1,303.68


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选择尺寸

变更视图
500 G
CN¥1,303.68
1 KG
CN¥1,738.93
5 KG
CN¥4,964.53

About This Item

UNSPSC代码:
12352203
NACRES:
NA.32

CN¥1,303.68


请联系客服了解存货情况

获取大包装报价

生物来源

mouse

偶联物

unconjugated

抗体形式

purified immunoglobulin (Purified IgG1 subclass)

抗体产品类型

primary antibodies

克隆

M2, monoclonal

表单

buffered aqueous solution (10 mM sodium phosphate, 150 mM NaCl, pH 7.4, containing 0.02% sodium azide)

纯化方式

using Protein A

种属反应性

all

浓度

3.8-4.2 mg/mL

技术

western blot: 10 μg/mL (Protein A)

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此商品
15604RDD034PHR1406
Urea BioXtra, pH 7.5-9.5 (20 °C, 5 M in H2O)

U0631

Urea

Urea puriss., meets analytical specification of Ph. Eur., BP, USP, 99.0-100.5%, 99.0-101.0% (calc. on dry substance)

15604

Urea

Urea free-flowing, Redi-Dri™, Bioreagent, ≥98%

RDD034

Urea

Urea Pharmaceutical Secondary Standard; Certified Reference Material

PHR1406

Urea

assay

98.5-101.5%

assay

99.0-100.5%, 99.0-101.0% (calc. on dry substance)

assay

≥98%

assay

-

application(s)

diagnostic assay manufacturing

application(s)

-

application(s)

-

application(s)

pharmaceutical (small molecule)

solubility

H2O: 5 M at 20 °C, clear, colorless

solubility

H2O: soluble 480 g/L at 20 °C

solubility

-

solubility

-

form

powder, crystals or pellets

form

solid

form

-

form

-

mp

132-135 °C (lit.)

mp

132-135 °C (lit.)

mp

132-135 °C (lit.)

mp

132-135 °C (lit.)

一般描述

抗-Flag M2抗体用于检测Flag融合蛋白质。该单克隆产生于小鼠中并可识别FLAG序列的N-末端、Met N-末端和C-末端。该抗体还可在内部位点识别FLAG。不同于M1,M2不是钙依赖性的。
F3165使用蛋白质A树脂进行亲和纯化,因此不仅含有抗-FLAG M2抗体,还有少量的原生小鼠IgG,这会增加它在大多数应用中的敏感性。
纯化方法——蛋白质A

免疫原

FLAG;肽序列DYKDDDDK

应用

小鼠产生的 ANTI-FLAG® M2 单克隆抗体已用于:
  • 免疫印迹
  • 免疫沉淀
  • 免疫细胞化学
  • 免疫荧光
  • ELISA
  • EIA
  • 染色质免疫沉淀
  • 电子显微分析
  • 流式细胞分析
  • 高速迁移测定

浏览我们有关其他应用的参考文献,请访问 FLAG® 文献页面。

制备说明

用3%脱脂牛奶在Tris缓冲盐溶液(TBS),pH 8.0中将抗体从0.5-10 ug/mL进行稀释

储存及稳定性

将稀释后的抗体分装并-20°C储存。不建议反复冻融。
注意:随着时间流逝,由于分子间的疏水相互作用,少量纯化抗体会从溶液中沉淀出来。如果在该产品中观察到沉淀,请短暂离心小瓶以复溶沉淀。从澄清上清液中取出所需体积的抗体溶液备用。这不会改变纯化抗体在蛋白质印迹或免疫沉淀应用中的性能。

法律信息

ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

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储存分类代码

12 - Non Combustible Liquids

WGK

nwg

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

常规特殊物品
  • 技术规格说明书

  • 历史批次信息供参考:

    分析证书(COA)

    Lot/Batch Number

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    Fangzhi Tan et al.
    Nature communications, 10(1), 3733-3733 (2019-08-21)
    Hearing loss is the most common sensory disorder. While gene therapy has emerged as a promising treatment of inherited diseases like hearing loss, it is dependent on the identification of gene delivery vectors. Adeno-associated virus (AAV) vector-mediated gene therapy has
    Jeong Gu Kang et al.
    Scientific reports, 9(1), 11960-11960 (2019-08-21)
    Despite the increased interest in epigenetic research, its progress has been hampered by a lack of satisfactory tools to control epigenetic factors in specific genomic regions. Until now, many attempts to manipulate DNA methylation have been made using drugs but
    T P Molitor et al.
    Oncogenesis, 2, e48-e48 (2013-06-05)
    The vaccinia-related kinases (VRKs) comprise a branch of the casein kinase family. VRK1, a ser/thr kinase with a nuclear localization, is the most well-studied paralog and has been described as a proproliferative protein. In lower eukaryotes, a loss of VRK1
    Casey C Fowler et al.
    Nature communications, 10(1), 3684-3684 (2019-08-17)
    Bacterial toxins with an AB5 architecture consist of an active (A) subunit inserted into a ring-like platform comprised of five delivery (B) subunits. Salmonella Typhi, the cause of typhoid fever, produces an unusual A2B5 toxin known as typhoid toxin. Here
    Takashi Fujitomo et al.
    Cancer research, 72(16), 4110-4118 (2012-06-22)
    Therapeutic targets for more effective and less toxic treatments of lung cancer remain important. Here we report the identification of the integral nuclear envelope protein TMEM209 as a critical driver of human lung cancer growth and survival. TMEM209 expression was

    商品

    比较anti-FLAG® M2磁珠的小规模FLAG®标签蛋白纯化的不同洗脱方法。

    Comparison of elution techniques for small-scale protein purification of FLAG® tag proteins using anti-FLAG® M2 magnetic beads.

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