产品名称
Chelating Sepharose™ Fast Flow, Cytiva 17-0575-01, pack of 50 mL
ligand
iminodiacetic acid
packaging
pack of 50 mL
manufacturer/tradename
Cytiva 17-0575-01
matrix
6% cross-linked agarose
particle size
45-165 μm
average diameter
90 μm
cleaning in place
2-14
working range
3-13
suitability
suitable for bioprocess medium
Analysis Note
Features and Benefits
- Uncharged IMAC resin allowing possibility to charge with your metal ion of choice for optimized selectivity
- Convenient purification of native and recombinant proteins
- As BioProcess medium it fulfills industrial demands for security of supply, robust performance and regulatory support.
- BioProcess medium supported for industrial applications and well-established in approved processes
General description
Chelating Sepharose™ Fast Flow is composed of cross-linked 6% agarose beads modified with iminodiacetic immobilized to the base matrix by stable ether linkages and sufficiently long spacer arms.
Chelating Sepharose™ Fast Flow is supplied free of metal ions, allowing the user to charge it with the most appropriate metal ion for purification of a target protein.
Chelating Sepharose™ Fast Flow is available in a range of different bulk pack sizes for easy scale-up and process development. As member of the BioProcess media range, Chelating Sepharose™ Fast Flow meets industrial demands with security of supply and comprehensive technical and regulatory support
Preparation Note
Legal Information
signalword
Warning
hcodes
存储类别
3 - Flammable liquids
法规信息
商品
This page shows how to solve practical problems that may occur when running an affinity chromatography column.
This page shows how to convert between flow velocity and volumetric flow rate in affinity chromatography of antibodies.
This page shows how to purify or remove proteins and peptides with exposed amino acids with Chelating Sepharose High Performance, Chelating Sepharose Fast Flow, Capto Chelating from Cytiva.
How to separate proteins and peptides with affinity for metal ions by immobilized metal chelate affinity chromatography.
实验方案
Guide for separating poly(His) fusion proteins using affinity chromatography tags in various purification products.
This page shows how to prepare samples for purification with affinity chromatography.
Sample Preparation for Affinity Chromatography in Specific Groups of Biomolecules
This page covers detailed information on cleaning procedures and recommended flow for column cleaning.
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