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重组
expressed in E. coli
质量水平
表单
lyophilized powder
比活
≥550 units/mg protein (biuret)
组成
Protein, 10-40% biuret
应用
agriculture
异质活性
creatine phosphokinase, glutathione reductase, myokinase, NADH oxidase, NADPH oxidase, phosphoglucomutase, 6-phosphogluconic dehydrogenase, phosphoglucose isomerase, lactic dehydrogenase, hexokinase ≤0.01%
储存温度
2-8°C
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相关类别
应用
- PGI 的偶联分光光度测定法（F6P-G6P 反应）
- 使用烟草叶研究淀粉时程
- 测定北美黑橡(Quercus velutina Lam. saplings)根和茎样品中的葡萄糖含量
- 使用野生型和转基因拟南芥整株幼苗的淀粉样本测定葡萄糖
生化/生理作用
单位定义
外形
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
dust mask type N95 (US), Eyeshields, Gloves
法规信息
历史批次信息供参考:
分析证书(COA)
Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
How do I find price and availability?
There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 or view local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
For Product No. G8529, Glucose-6-phosphate Dehydrogenase from Leuconostoc mesenteroides, can the Ficoll present in the product be removed?
Ficoll is added to the enzyme preparation as a stabilizer. The enzyme may become unstable if the Ficoll is removed (i.e., through a dialysis step).
In which solvent can Product No. G8529, Glucose- 6-phosphate Dehydrogenase from Leuconostoc mesenteroides, be dissolved?
The product can be dissolved at 1 mg/ml in 5 mM glycine buffer, pH 8, (with or without 0.1% BSA) or in deionized water at 1 mg/ml (buffer is preferable)
Can solutions of Product No. G8529, Glucose-6-phosphate Dehydrogenase, recombinant from Leuconostoc mesenteroides, be stored?
Sigma has not evaluated the solution stability of this enzyme. Perhaps solutions in buffer may be aliquotted and stored frozen at 20°C for about 1-2 months. Sigma offers the enzyme in an ammonium sulfate suspension, product G8404, for solution storage at 2-8°C.
What are the differences between Product No. G8529, Glucose-6-phosphate Dehydrogenase and Product No. G5885, Glucose-6-phosphate Dehydrogenase?
Product No. G8529 is a recombinant protein from Leuconostoc mesenteroides, expressed in Escherichia coli. Product No. G5885 is a non-recombinant protein from Leuconostoc mesenteroides.
What is the molecular weight of Product No. G8529, Glucose-6-phosphate Dehydrogenase?
Sigma has not determined the molecular weight of this protein, but a value of 55,000 daltons has been reported for the monomer molecular weight (the enzyme from Leuconostoc mesenteroides is reportedly a dimer protein) (Biochem. Biophys. Res. Comm. 59, 894 (1974)).
My question is not addressed here, how can I contact Technical Service for assistance?
Ask a Scientist here.
实验方案
To measure glucose-6-phosphate dehydrogenase activity, beta-nicotinamide adenine dinucleotide phosphate is used in a spectrophotometric rate determination assay at 340 nm.
在测定6-磷酸葡萄糖脱氢酶活性时，使用β-烟酰胺腺嘌呤二核苷酸磷酸在340 nm处通过分光光度法进行测定。
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