跳转至内容
Merck
CN
所有图片(1)

主要文件

查看所有文档

安全信息

G8404

Sigma-Aldrich

葡萄糖-6-磷酸脱氢酶 来源于肠系膜明串珠菌

recombinant, expressed in E. coli, ammonium sulfate suspension, ≥550 units/mg protein (biuret)

别名:

Entner-Doudoroff酶, G6PD, G6PDH, NADP 葡萄糖 6-磷酸脱氢酶, G-6-P-DH

登录查看公司和协议定价
所有图片(1)

About This Item

CAS号:
9001-40-5
EC 号:
1.1.1.49 (BRENDA, IUBMB)
MDL编号:
MFCD00081656
UNSPSC代码:
12352204
NACRES:
NA.54

生物来源

bacterial (Leuconostoc mesenteroides)

质量水平

300

重组

expressed in E. coli

表单

ammonium sulfate suspension

比活

≥550 units/mg protein (biuret)

分子量

128 kDa

储存条件

(Tightly closed)

技术

cell culture | mammalian: suitable

UniProt登记号

UPI0021A4D550

异质活性

creatine phosphokinase, glutathione reductase, myokinase, NADH oxidase, NADPH oxidase, phosphoglucomutase, 6-phosphogluconic dehydrogenase, phosphoglucose isomerase, lactic dehydrogenase, hexokinase ≤0.01%

储存温度

2-8°C

正在寻找类似产品? 访问 产品对比指南

相关类别

一般描述

葡萄糖6-磷酸脱氢酶（G-6-P-DH）是磷酸戊糖途径第一步的关键调节酶。G-6-P-DH是一种糖蛋白，分子量为 128 kDa（凝胶过滤）。G6PD 是二聚体，每个亚基上有一个活性位点。

研究领域：细胞信号传导

应用

葡萄糖-6-磷酸脱氢酶已用于：

  • 测试玉米胚乳发育过程中的酮糖还原酶活性。
  • 测定冠心病患者血清中甘露糖的水平
  • 研究其对细胞外聚合物 (EPS) 提取物的反应活性，超声法测定细胞裂解液
  • 测定培养人体肌肉卫星细胞的葡萄糖摄入情况

生化/生理作用

G6PD可为所有细胞提供还原动力（如烟酰胺腺嘌呤二核苷酸磷酸 (NADPH)），使细胞能够平衡氧化压力。G6PD基因突变与 X 连锁G6PD缺陷有关——这是一种遗传性基因缺陷，包括新生儿黄疸和急性溶血性贫血等。
葡萄糖-6-磷酸脱氢酶(G6PD)催化葡萄糖-6-磷酸转化为6-磷酸葡糖酸内酯，作为磷酸戊糖途径的第一步。

单位定义

在 NAD 存在下，在pH 7.8，30°C 下，1 个单位将每分钟氧化 1.0 μmol D-6-磷酸葡萄糖至 6-磷酸-D-葡萄糖酸盐。

外形

用 3.2 M (NH 4 ) 2 SO 4 （含 50 mM Tris 和 1 mM MgCl 2 ，pH 7.5）混悬

其他说明

The volume is lot specific and can be calculated using the data:
For example, lot 0000114274
Units per mg Protein = 846
Mg Protein per mL - 9
Units per mL = 7614
G8404-1000U = approx 132 ul

储存分类代码

12 - Non Combustible Liquids

WGK

WGK 2

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type ABEK (EN14387) respirator filter

法规信息

常规特殊物品

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000410707
0000410707
0000367244
0000367243
0000367241

没有发现合适的版本？

如果您需要特殊版本，可通过批号或批次号查找具体证书。

搜索COA

已有该产品？

在文件库中查找您最近购买产品的文档。

访问文档库

Angelo Minucci et al.
IUBMB life, 61(1), 27-34 (2008-10-23)
Glucose 6-phosphate dehydrogenase (G6PD) deficiency is the most common defect of red blood cells. Although some different laboratory techniques or methods are employed for the biochemical screening, a strict relationship between biochemists, clinicians, and molecular biologists is necessary for a
Charles-David Dubé et al.
AMB Express, 9(1), 23-23 (2019-02-08)
Extracellular polymeric substances (EPS) play major roles in the efficacy of biofilms such as anaerobic granules, ranging from structural stability to more specific functions. The EPS of three granular anaerobic sludges of different origins were studied and compared. Particularly, the
Hemang M Parikh et al.
BMC endocrine disorders, 21(1), 32-32 (2021-03-01)
Insulin resistance (IR) in skeletal muscle is a key feature of the pre-diabetic state, hypertension, dyslipidemia, cardiovascular diseases and also predicts type 2 diabetes. However, the underlying molecular mechanisms are still poorly understood. To explore these mechanisms, we related global
Cara A Griffiths et al.
Nature, 540(7634), 574-578 (2016-12-16)
The pressing global issue of food insecurity due to population growth, diminishing land and variable climate can only be addressed in agriculture by improving both maximum crop yield potential and resilience. Genetic modification is one potential solution, but has yet
M D Cappellini et al.
Lancet (London, England), 371(9606), 64-74 (2008-01-08)
Glucose-6-phosphate dehydrogenase (G6PD) deficiency is the most common human enzyme defect, being present in more than 400 million people worldwide. The global distribution of this disorder is remarkably similar to that of malaria, lending support to the so-called malaria protection
查看所有相关文献

商品

如何使用溶于硫酸铵悬液的酶

以硫酸铵悬浮液形式提供的酶的使用指南

How to Work with Enzymes Supplied as Ammonium Sulfate Suspensions

Instructions for working with enzymes supplied as ammonium sulfate suspensions

实验方案

6-磷酸葡萄糖脱氢酶（EC 1.1.1.49）的酶学测定

在测定6-磷酸葡萄糖脱氢酶活性时，使用β-烟酰胺腺嘌呤二核苷酸磷酸在340 nm处通过分光光度法进行测定。

Enzymatic Assay of Glucose-6-Phosphate Dehydrogenase (EC 1.1.1.49)

To measure glucose-6-phosphate dehydrogenase activity, beta-nicotinamide adenine dinucleotide phosphate is used in a spectrophotometric rate determination assay at 340 nm.

我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

联系技术服务部门