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Merck
CN

E8263

Benzonase®核酸酶，超纯

≥250 units/μL, ≥99% (SDS-PAGE), recombinant, expressed in E. coli, buffered aqueous glycerol solution, ultrapure grade

别名:

内切核酸酶 来源于粘质沙雷氏菌

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关于此项目

化学文摘社编号:
9025-65-4
UNSPSC Code:
12352204
NACRES:
NA.54
EC 号:
3.1.30.2 (BRENDA, IUBMB)
MDL number:
MFCD00131010

主要文件

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产品名称

Benzonase®核酸酶，超纯, ≥250 units/μL, ≥99% (SDS-PAGE), recombinant, expressed in E. coli, buffered aqueous glycerol solution, ultrapure grade

biological source

Serratia marcescens

recombinant

expressed in E. coli

grade

ultrapure grade

assay

≥99% (SDS-PAGE)

form

buffered aqueous glycerol solution

mol wt

30 kDa

concentration

≥250 units/μL

application(s)

research use

shipped in

wet ice

storage temp.

−20°C

Quality Level

200

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相关类别

Application

用于去除蛋白样品中的核酸。
该酶已用于一项检测不受Benzonase核酸酶降解的Tf1 RNA量的检测中。粟酒裂殖酵母的Tf1元件是含有长末端重复序列的反转录转座子，可编码功能性蛋白酶、逆转录酶和整合酶蛋白。它还用于从细胞裂解物中获得的核颗粒和核提取物的分馏。
超纯的Benzonase核酸酶，或来自粘质沙雷氏菌的内切核酸酶，已用于一项评估十二烷基苯磺酸钠和消泡剂对粘质沙雷氏菌内切核酸酶活性影响的研究中。来自粘质沙雷氏菌的内切核酸酶已用于一项探究染色质亚基结构的研究中。

Biochem/physiol Actions

Benzonase®是一种来自粘质沙雷氏菌的基因工程改造内切核酸酶。该蛋白质是30kDa亚基的二聚体，带有两个必需的二硫键。该内切核酸酶可攻击并降解所有形式的DNA和RNA（单链的、双链的、线性的和环化的），并可在一系列宽泛的操作条件下具有高效性。它可将核酸完全消化为长度为3至5个碱基的5′-单磷酸末端寡核苷酸。这是从重组蛋白中去除核酸的理想选择。它还可用于需要完全消化核酸的应用。它还可降低蛋白提取物的粘度并防止细胞成团。用这种酶对蛋白样品进行预处理可通过去除任何结合的核酸而提高它在2D凝胶电泳中的分辨率。最适的酶活pH为8.0-9.2。
消化天然或热变性的DNA和RNA。

Legal Information

Benzonase®核酸酶由德国达姆施塔特默克公司和/或其附属公司提供。
Benzonase is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

在pH 8.0，37 °C条件下，一个单位的酶在30分钟内可将超声的鲑鱼精子DNA消化成等同于1.0 ΔA260的酸可溶寡核苷酸（2.625 ml反应体积）。

Physical form

在含有20 mM Tris HCl, pH 8.0, 2 mM MgCl2及20 mM NaCl的50%甘油中的溶液。

存储类别

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves

法规信息

常规特殊物品
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历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000464345
0000464345
0000353472
0000353472
0000338703

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Syed Nabeel-Shah et al.
Scientific reports, 10(1), 168-168 (2020-01-15)
Chromatin organization influences most aspects of gene expression regulation. The linker histone H1, along with the core histones, is a key component of eukaryotic chromatin. Despite its critical roles in chromatin structure and function and gene regulation, studies regarding the
Laure Teysset et al.
Journal of virology, 77(9), 5451-5463 (2003-04-15)
The Tf1 element of Schizosaccharomyces pombe is a long terminal repeat-containing retrotransposon that encodes functional protease, reverse transcriptase, and integrase proteins. Although these proteins are known to be necessary for protein processing, reverse transcription, and integration, respectively, the function of
T K Ball et al.
Nucleic acids research, 20(19), 4971-4974 (1992-10-11)
The role of the two disulfide bonds found in the Serratia marcescens nuclease were tested by site directed mutagenesis and were found essential for nuclease activity, although slight residual activity remained. The requirement for disulfide bond formation may play a
T K Ball et al.
Gene, 57(2-3), 183-192 (1987-01-01)
We are studying exoproteins of the enteric bacterium Serratia marcescens as a model system for the release of extracellular proteins from the cell. In this work we report the cloning of the gene for a secreted nuclease from S. marcescens
An extracellular nuclease from Serratia marcescens. II. Specificity of the enzyme.
M Nestle et al.
The Journal of biological chemistry, 244(19), 5219-5225 (1969-10-10)
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