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D9779

Sigma-Aldrich

DL-二硫苏糖醇

for molecular biology, ≥98% (HPLC), ≥99% (titration)

别名:

(±)-二硫苏糖醇，外消旋二硫苏糖醇，二硫苏糖醇, 1,4-二巯基苏糖醇, DTT, 克莱兰氏试剂

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About This Item

线性分子式:
HSCH2CH(OH)CH(OH)CH2SH
CAS号:
3483-12-3
分子量:
154.25
Beilstein:
1719757
EC 号:
222-468-7
MDL编号:
MFCD00004877
UNSPSC代码:
12161504
PubChem化学物质编号:
24894330
NACRES:
NA.31

等级

for molecular biology

质量水平

200

检测方案

≥98% (HPLC)
≥99% (titration)

形式

powder

反应适用性

reagent type: reductant

mp

41-44 °C (lit.)

溶解性

H2O: 50 mg/mL

痕量阳离子

heavy metals (as Pb): ≤5 ppm

适用性

suitable for molecular biology

异质活性

DNase, RNase, protease, none detected

储存温度

2-8°C

SMILES字符串

O[C@H](CS)[C@H](O)CS

InChI

1S/C4H10O2S2/c5-3(1-7)4(6)2-8/h3-8H,1-2H2/t3-,4-/m1/s1

InChI key

VHJLVAABSRFDPM-QWWZWVQMSA-N

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相关类别

一般描述

二硫苏糖醇 (DTT) 是维持蛋白质中 SH 基团还原态的优异试剂。它可引发硫醇-二硫键交换反应，完全还原生物分子中的分子内或分子间二硫键。DTT 专门靶向 N,N′-双(丙烯酰)胱胺交联剂的二硫键，分解聚丙烯酰胺凝胶和十二烷基聚丙烯酰胺钠凝胶电泳 (SDS-PAGE) 基质。因此，其与定量还原二硫化物的缓冲液联用时非常有效。DTT 的刺激性和毒性低于2-巯基乙醇，浓度也达到β-巯基乙醇的7倍。

应用

DL-二硫苏糖醇用于蛋白质样品中，以在将样品上样到凝胶上进行蛋白质印迹或 SDS-PAGE 之前还原二硫键它已用于 mRNA 原位杂交过程。

这一物质可用于：
  • 在蛋白质印迹分析中用作蛋白提取的组分
  • 配制用于定量质谱分析的样品裂解缓冲液
  • 用作酶联免疫吸附测定 (ELISA) 的激酶缓冲液成分
用于将 −SH 基保持在还原态的绝佳试剂；定量还原二硫化物。在 SDS-PAGE 前，DTT 在样品缓冲液中可有效还原蛋白二硫键。DTT 还可用于还原交联剂 N,N′-双(丙烯酰)胱胺的二硫键桥以分解聚丙烯酰胺凝胶基质。DTT 的刺激性和毒性都低于 2-巯基乙醇。通常，使用 DTT (100mM) 所需的浓度是使用 2-巯基乙醇 (5% v/v, 700mM) 的浓度的七分之一。

生化/生理作用

二硫苏糖醇 (DTT)常用于硫醇蛋白的生化制备和化学肽合成。DTT还用于蛋白质折叠和酶活性的蛋白质化学研究。

特点和优势

  • 适用于分子生物学
  • 不含RNA酶、DNA酶、核酸外切酶、蛋白酶
  • 高纯度(HPLC ≥98%)
  • 不含重金属 ≤5ppm

其他说明

如需了解生化试剂系列的更多信息，请填写此表。

象形图

CorrosionExclamation mark
GHS05,GHS07

警示用语：

Danger

危险声明

H302,H315,H318

危险分类

Acute Tox. 4 Oral - Eye Dam. 1 - Skin Irrit. 2

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ （Lot或Batch）字后找到。

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. What is the shelf life of Product D9779, DL-Dithiothreitol?

    The recommended retest period for D9779 is 4 years. This information can be found on the Specification Sheet. The actual retest date for a given lot can be found on the Certificate of Analysis for that lot.

  4. What is the solubility of Product D9779, DL-Dithiothreitol?

    This product is soluble in water (50 mg/mL), yielding a clear, colorless solution. DTT is also soluble in ethanol, acetone, ethyl acetate, chloroform and ether. This information may be found on the product information sheet for a related DTT product (Product No. D0632).

  5. What is the solution stability of Product D9779, DL-Dithiothreitol (DTT)?

    We generally recommend preparing DTT solutions fresh daily. A table showing the half life of DTT in solution under various conditions can be found on the product information sheet for a related DTT product (Product No. D0632).

  6. What is the meaning of Molecular Biology Grade in the description of Product D9779, DL-Dithiothreitol?

    Molecular Biology grade products are tested for DNase, RNase, and protease to make certain that they contain minimal levels of these contaminants.

  7. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  8. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  9. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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实验方案

Oligo Reduction

Follow this DDT reduction protocol to reduce disulfide bonds in thiol-modified oligonucleotides.

寡核苷酸还原

根据此DTT还原实验方案来还原硫醇修饰的寡核苷酸中的二硫键。

我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

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