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D0632

Sigma-Aldrich

DL-二硫苏糖醇

≥98% (HPLC), ≥99.0% (titration)

别名:

(±)-二硫苏糖醇，外消旋二硫苏糖醇，二硫苏糖醇, 1,4-二巯基苏糖醇, DTT, 克莱兰氏试剂

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CN¥9,493.39

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About This Item

线性分子式:
HSCH2CH(OH)CH(OH)CH2SH
CAS号:
3483-12-3
分子量:
154.25
Beilstein:
1719757
EC 号:
222-468-7
MDL编号:
MFCD00004877
UNSPSC代码:
12352201
PubChem化学物质编号:
24893251
NACRES:
NA.21

CN¥9,493.39

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质量水平

200

方案

≥98% (HPLC)
≥99.0% (titration)

表单

powder

反应适用性

reagent type: reductant

颜色

white

mp

41-44 °C (lit.)

溶解性

H2O: soluble 50 mg/mL, clear, colorless to very faintly yellow

应用

general analytical

储存温度

2-8°C

SMILES字符串

O[C@H](CS)[C@H](O)CS

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正己烷 suitable for chromatography

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technique(s)

GC/MS: suitable

technique(s)

HPLC: suitable

technique(s)

-

technique(s)

UV/Vis spectroscopy: suitable

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100

assay

≥98% (GC)

assay

≥98% (GC)

assay

-

assay

≥99% (GC)

solubility

0.0095 g/L

solubility

-

solubility

-

solubility

-

form

liquid

form

liquid

form

-

form

liquid

vapor pressure

256 mmHg ( 37.7 °C), ~132 mmHg ( 20 °C), 5.2 psi ( 37.7 °C)

vapor pressure

256 mmHg ( 37.7 °C), 5.2 psi ( 37.7 °C), ~132 mmHg ( 20 °C)

vapor pressure

256 mmHg ( 37.7 °C), 5.2 psi ( 37.7 °C), ~132 mmHg ( 20 °C)

vapor pressure

256 mmHg ( 37.7 °C), 5.2 psi ( 37.7 °C), ~132 mmHg ( 20 °C)

一般描述

二硫苏糖醇 (DTT) 是一种存在于蛋白质和氨基酸半胱氨酸中的二硫键 (SH) 还原剂。它可形成六膜环并引发硫醇-二硫键交换，从而还原二硫键。它的还原能力几乎是βME的 7 倍，因此在较低浓度下也能发挥作用。

应用

DTT已用于：α
  • 作为牛奶中主要过敏原αs1-酪蛋白的还原和烷基化反应物之一。[1]
  • 作为精子去膜和再激活培养基的一个组分。[2]
  • 因硫醇可有效保护生物催化剂的活性位点而维持酶的稳定性。[3]
  • 作为还原剂用于测试N-乙基马来酰亚胺与巯基反应的特异性。[4]
  • 蛋白质组学分析，作为质谱分析的溶液蛋白质消化物质[5]
  • 作为蛋白质定量的缓冲液成分，制备洗涤缓冲液 、裂解缓冲液、样品缓冲液和蛋白质洗脱缓冲液[6][4]
用于将 −SH 基保持在还原态的绝佳试剂；定量还原二硫化物。在 SDS-PAGE 前，DTT 在样品缓冲液中可有效还原蛋白二硫键。DTT 还可用于还原交联剂 N,N′-双(丙烯酰)胱胺的二硫键桥以分解聚丙烯酰胺凝胶基质。DTT 的刺激性和毒性都低于 2-巯基乙醇。通常，使用 DTT (100mM) 所需的浓度是使用 2-巯基乙醇 (5% v/v, 700mM) 的浓度的七分之一。

生化/生理作用

二硫苏糖醇 (DTT) 广泛用于化学肽合成及硫醇蛋白的生化制备。此外，该物质还用于各类蛋白质化学研究，例如蛋白质折叠和酶活性。另外，它还可作为避免 DNA 修饰的酶稳定剂。DTT 特异性介导硫醇-二硫键交换反应，以完全还原生物分子中的分子内或分子间二硫键。从而形成硫醇和 DTT 的环状二硫化物。[7]

特点和优势

  • 高品质DTT (HPLC≥98%)、（滴度≥99.0%）
  • 适于电泳、蛋白质组学分析

其他说明

如需了解生化试剂系列的更多信息，请填写此表。

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象形图

CorrosionExclamation mark
GHS05,GHS07

警示用语：

Danger

危险声明

H302,H315,H318

危险分类

Acute Tox. 4 Oral - Eye Dam. 1 - Skin Irrit. 2

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

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    0000433203
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    0000433205
    0000433204
    0000433204

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    O Linhart et al.
    Reproduction (Cambridge, England), 124(5), 713-719 (2002-11-06)
    This study investigated the effects of different environmental conditions on the motility parameters of paddlefish (Polyodon spathula) spermatozoa. Paddlefish spermatozoa demonstrated the following characteristics: (i) all spermatozoa were motile 10 s after activation with a velocity of 130-160 microm s(-1);
    Florian Füssl et al.
    mAbs, 11(1), 116-128 (2018-10-09)
    Charge variant analysis is a widely used tool to monitor changes in product quality during the manufacturing process of monoclonal antibodies (mAbs). Although it is a powerful technique for revealing mAb heterogeneity, an unexpected outcome, for example the appearance of
    R Quiles et al.
    Clinical chemistry, 39(3), 500-503 (1993-03-01)
    An automated method based on the principles of flow-injection analysis is proposed for the enzymatic determination of sodium ion in serum. The method relies on the activation of beta-galactosidase by the analyte. The features of the proposed method include linear
    Daniel C Marcus et al.
    Auditory neuroscience, 1, 101-109 (1994-01-01)
    Vestibular dark cell epithelium was isolated from the semicircular canal of gerbils to test the proposal that the sulfhydryl alkylating agent N-ethylmaleimide (NEM) inhibits K(+) secretion by this tissue and does so by reacting with a site in or near
    Niamh McNamee et al.
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