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质量水平
检测方案
≥98% (HPLC)
≥99.0% (titration)
形式
powder
反应适用性
reagent type: reductant
颜色
white
mp
41-44 °C (lit.)
溶解性
H2O: soluble 50 mg/mL, clear, colorless to very faintly yellow
应用
general analytical
储存温度
2-8°C
SMILES字符串
O[C@H](CS)[C@H](O)CS
InChI
1S/C4H10O2S2/c5-3(1-7)4(6)2-8/h3-8H,1-2H2/t3-,4-/m1/s1
InChI key
VHJLVAABSRFDPM-QWWZWVQMSA-N
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一般描述
二硫苏糖醇 (DTT) 是一种存在于蛋白质和氨基酸半胱氨酸中的二硫键 (SH) 还原剂。它可形成六膜环并引发硫醇-二硫键交换，从而还原二硫键。它的还原能力几乎是βME的 7 倍，因此在较低浓度下也能发挥作用。
应用
DTT已用于：α
- 作为牛奶中主要过敏原αs1-酪蛋白的还原和烷基化反应物之一。
- 作为精子去膜和再激活培养基的一个组分。
- 因硫醇可有效保护生物催化剂的活性位点而维持酶的稳定性。
- 作为还原剂用于测试N-乙基马来酰亚胺与巯基反应的特异性。
- 蛋白质组学分析，作为质谱分析的溶液蛋白质消化物质
- 作为蛋白质定量的缓冲液成分，制备洗涤缓冲液 、裂解缓冲液、样品缓冲液和蛋白质洗脱缓冲液
用于将 −SH 基保持在还原态的绝佳试剂；定量还原二硫化物。在 SDS-PAGE 前，DTT 在样品缓冲液中可有效还原蛋白二硫键。DTT 还可用于还原交联剂 N,N′-双(丙烯酰)胱胺的二硫键桥以分解聚丙烯酰胺凝胶基质。DTT 的刺激性和毒性都低于 2-巯基乙醇。通常，使用 DTT (100mM) 所需的浓度是使用 2-巯基乙醇 (5% v/v, 700mM) 的浓度的七分之一。
生化/生理作用
二硫苏糖醇 (DTT) 广泛用于化学肽合成及硫醇蛋白的生化制备。此外，该物质还用于各类蛋白质化学研究，例如蛋白质折叠和酶活性。另外，它还可作为避免 DNA 修饰的酶稳定剂。DTT 特异性介导硫醇-二硫键交换反应，以完全还原生物分子中的分子内或分子间二硫键。从而形成硫醇和 DTT 的环状二硫化物。
特点和优势
- 高品质DTT (HPLC≥98%)、（滴度≥99.0%）
- 适于电泳、蛋白质组学分析
其他说明
For additional information on our range of Biochemicals, please complete this form.
可比产品
产品编号
说明
价格
建议的防溅射手套
警示用语：
Danger
危险声明
危险分类
Acute Tox. 4 Oral - Eye Dam. 1 - Skin Irrit. 2
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
Daniel C Marcus et al.
Auditory neuroscience, 1, 101-109 (1994-01-01)
Vestibular dark cell epithelium was isolated from the semicircular canal of gerbils to test the proposal that the sulfhydryl alkylating agent N-ethylmaleimide (NEM) inhibits K(+) secretion by this tissue and does so by reacting with a site in or near
O Linhart et al.
Reproduction (Cambridge, England), 124(5), 713-719 (2002-11-06)
This study investigated the effects of different environmental conditions on the motility parameters of paddlefish (Polyodon spathula) spermatozoa. Paddlefish spermatozoa demonstrated the following characteristics: (i) all spermatozoa were motile 10 s after activation with a velocity of 130-160 microm s(-1);
R Quiles et al.
Clinical chemistry, 39(3), 500-503 (1993-03-01)
An automated method based on the principles of flow-injection analysis is proposed for the enzymatic determination of sodium ion in serum. The method relies on the activation of beta-galactosidase by the analyte. The features of the proposed method include linear
Florian Füssl et al.
mAbs, 11(1), 116-128 (2018-10-09)
Charge variant analysis is a widely used tool to monitor changes in product quality during the manufacturing process of monoclonal antibodies (mAbs). Although it is a powerful technique for revealing mAb heterogeneity, an unexpected outcome, for example the appearance of
S Elsayed et al.
Scandinavian journal of immunology, 60(5), 486-493 (2004-11-16)
Alphas1-Casein (CAS1_BOVIN), the major allergen of cow's milk (CM), is widely used as hydrolysates in infant diet formulae and additive to other processed food items. To date, most of the reported B-cell epitope mapping were performed on polyethylene pins or
商品
Compare columns in resolving medium-sized antibody fragments after digestion with DTT or IdeS using Reversed-Phase Chromatography for analysis.
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