所有图片(1)
About This Item
经验公式（希尔记法）:
C4H10O2S2
CAS号:
分子量:
154.25
Beilstein:
1719757
MDL编号:
UNSPSC代码:
12161504
PubChem化学物质编号:
NACRES:
NA.25
形式
liquid
质量水平
反应适用性
reagent type: reductant
浓度
1 M in H2O
密度
1.04 g/mL at 20 °C
1.035 g/mL at 25 °C
SMILES字符串
O[C@H](CS)[C@H](O)CS
InChI
1S/C4H10O2S2/c5-3(1-7)4(6)2-8/h3-8H,1-2H2/t3-,4-/m1/s1
InChI key
VHJLVAABSRFDPM-QWWZWVQMSA-N
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一般描述
二硫苏糖醇是用于蛋白质分析的一种有效还原剂。在制备用于电泳或其他应用的样品时，密封安瓿形式更加方便且易于使用，并且可避免浪费和效力损失。
二硫苏糖醇（DTT）在蛋白质组学应用中用于保护还原态中的巯基（-SH），也用于二硫基（-S-S-）基团的定量还原。由于其能够还原蛋白质样品的氧化态并保留酶活性，它是一种常用的缓冲液试剂。在溶液中其它二硫化物的还原过程中，DTT被氧化为环状二硫化物。
应用
DL-二硫苏糖醇溶液已被用于：
- 蛋白质印迹
- 作为裂解缓冲液的增补剂用于研究其对精子裂解的作用
- 作为冷冻均质缓冲液和冷冻冲洗缓冲液的成分用于分离冷冻大鼠组织的细胞核
- 十二烷基硫酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)、色谱、含半胱氨酸化合物的修饰
特点和优势
与其它硫醇化合物相比具有低毒性和减少的异味。
警示用语：
Danger
危险声明
危险分类
Eye Dam. 1 - Skin Irrit. 2
WGK
WGK 2
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter
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