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Merck
CN

D8900

杜氏改良Eagle 培养基/营养混合物 F-12 Ham

With L-glutamine and 15 mM HEPES, without sodium bicarbonate, powder, suitable for cell culture

别名:

DME/F-12, 1:1混合

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关于此项目

UNSPSC Code:
12352207
NACRES:
NA.71
MDL number:
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Quality Level

form

powder

technique(s)

cell culture | mammalian: suitable

components

sodium pyruvate: 0.055 g/L
HEPES: 15 mM
NaHCO3: no
L-glutamine: yes
glucose: 3.15 g/L
phenol red: yes

shipped in

ambient

storage temp.

2-8°C

Application

Dulbecco改良Eagle培养基/Ham F-12营养混合物适合低血清条件下广泛的细胞类型生长。已用于:

  • 作为培养基组分用于子宮內膜外植体的体外培养
  • 浸渍壳聚糖-明胶-甘油磷酸水凝胶,研究细胞毒性
  • 培养髓核(NP)细胞以培养人关节软骨细胞,评估黃芩素毒性

Preparation Note

补充 1.2 g/L 碳酸氢钠。
配制成每升培养基含 15.6 克粉末。


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存储类别

11 - Combustible Solids

wgk

WGK 1



历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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实验方案

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

粉末培养基及盐混合物极具吸湿性,应注意防潮。

商品

Nutrient Mixture F-12 Ham Formulation

High-quality ultrapure water that is endotoxin-free is recommended for mammalian cell culture as it support cell viability and prevents contamination.


Ryosuke Sakumoto et al.
Journal of animal science and biotechnology, 9, 46-46 (2018-06-28)
The aim of the present study was to evaluate CCL8 and CXCL10 expression and its regulatory mechanism in peripheral blood leukocytes (PBLs) at the time of maternal recognition in cows. Blood samples were collected on 14, 15, 16, 17 and
Elena Ruggeri et al.
Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada, 23(6), 1197-1206 (2017-12-07)
Confocal microscopy was used to image stages of equine zygote development, at timed intervals, after intracytoplasmic sperm injection (ICSI) of oocytes that were matured in vivo or in vitro. After fixation for 4, 6, 8, 12, or 16 h after
Sang Hwan Kim et al.
PeerJ, 7, e6344-e6344 (2019-02-08)
Here we investigated the expressions of apoptosis-associated genes known to induce programed cell death through mRNA expressions of two matrix metalloproteinases (MMPs) that are involved in the degradation of collagen and basal membrane in luteal cells cultured in the treatment



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