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Merck
CN

D5648

Sigma-Aldrich

DMEM - high glucose

With ʟ-glutamine, without sodium bicarbonate and sodium pyruvate, powder, suitable for cell culture

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别名:
DME, DMEM
UNSPSC代码:
12352207
NACRES:
NA.75

product name

杜氏改良 Eagle 培养基 - 高葡萄糖, With 4500 mg/L glucose and L-glutamine, without sodium bicarbonate, powder, suitable for cell culture

形式

powder

技术

cell culture | mammalian: suitable

组分

phenol red: yes
L-glutamine: yes
NaHCO3: no
HEPES: no
glucose: high
sodium pyruvate: no

运输

ambient

储存温度

2-8°C

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应用

Dulbecco′s 改良Eagles 培养基- 高葡萄糖已被用于各种细胞类型的培养基,包括人宫颈内上皮End1/E6E7细胞、人腺癌上皮细胞(HeLa)、人肺泡腺癌上皮细胞(A549)、人骨肉瘤上皮细胞、人二倍体细胞系、原代肝细胞和滑膜组织
Dulbecco′s Modified Eagle′s Medium(DMEM)是对Basal Medium Eagle (BME)的一种改良,含有四倍浓度的氨基酸和维生素。 原始的配方含有1000 mg/L的葡萄糖并用于培养小鼠胚胎细胞。 从此,它被按照多种方式进行修改以支持小鼠和鸡细胞的原代培养,以及多种正常和转化细胞。 这些培养基都可提供一种不同的L-谷氨酰胺和丙酮酸钠组成。 此外,葡萄糖浓度也被提高至4500 mg/L,因此被命名为“DMEM/High”。

数量

按照每升培养基13.4克粉末配制。

重悬

补充3.7g/L碳酸氢钠。

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

常规特殊物品

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商品

Cell culture protocol for passaging and splitting suspension cell lines

用于传代和分装悬浮细胞系的细胞培养方案

实验方案

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

粉末培养基及盐混合物极具吸湿性,应注意防潮。

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