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product name
杜氏改良Eagle 培养基/营养混合物 F-12 Ham, With L-glutamine and 15 mM HEPES, without sodium bicarbonate and phenol red, powder, suitable for cell culture
质量水平
形式
powder
技术
cell culture | mammalian: suitable
组分
NaHCO3: no
L-glutamine: yes
phenol red: no
sodium pyruvate: 0.055 g/L
glucose: 3.15 g/L
HEPES: 15 mM
运输
ambient
储存温度
2-8°C
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一般描述
Dulbecco改良Eagle培养基/Ham F-12营养混合物适合低血清条件下广泛的细胞类型生长。克隆和干细胞培养的研究者通常更喜欢去除酚红。
应用
Dulbecco改良Eagle培养基/Ham F-12营养混合物已用于培养：
- Hep-2细胞
- 小鼠成纤维L‐细胞
- L‐ Wnt家族成员3A(Wnt3a)细胞
- 原代水牛胚胎成纤维细胞(BEF)
数量
配方中每升培养基含有15.6克的粉末。
重悬
并补充1.2 g/L碳酸氢钠。
补充剂
通常也和此产品一起购买
产品编号
说明
价格
WGK
WGK 1
法规信息
动植物来源培养基
Tao Fu et al.
FEBS letters, 593(15), 2040-2050 (2019-06-04)
Oncostatin M (OSM) is a member of the interleukin-6 (IL-6) family, which functions in embryo implantation and decidualization. The expression, function and regulation of Osm in mouse uteri during early pregnancy remain unknown. We show that Osm is mainly expressed
Nereo Kalebic et al.
EMBO reports, 17(3), 338-348 (2016-01-14)
We have applied the CRISPR/Cas9 system in vivo to disrupt gene expression in neural stem cells in the developing mammalian brain. Two days after in utero electroporation of a single plasmid encoding Cas9 and an appropriate guide RNA (gRNA) into
C Zhang et al.
Osteoarthritis and cartilage, 26(6), 824-833 (2018-03-20)
Adipokines play roles in the pathogenesis of osteoarthritis (OA). Fatty acid binding protein 4 (FABP4) is a novel adipokine that is closely associated with obesity and metabolic diseases. The aim of this study was to discover the potential role of
Peter S B Finnie et al.
Current biology : CB, 30(10), 1934-1940 (2020-04-04)
Memory allows organisms to predict future events based on their prior sampling of the world. Rather than faithfully encoding each detail of related episodes, the brain is thought to incrementally construct probabilistic estimates of environmental statistics that are re-evaluated each
Dristi Bhandari et al.
Journal of cellular biochemistry, 122(10), 1262-1276 (2021-05-14)
WNT5A activates noncanonical Wnt signaling pathways and has critical functions in early development, differentiation, and tissue homeostasis. Two major WNT5A protein isoforms, which in this study we term WNT5A-L(A) and WNT5A-S(B), have been identified that differ by 18 AA at
商品
Nutrient Mixture F-12 Ham Formulation
实验方案
Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.
粉末培养基及盐混合物极具吸湿性，应注意防潮。
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