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Merck
CN

D0547

Sigma-Aldrich

杜氏改良Eagle 培养基/营养混合物 F-12 Ham

With L-glutamine and trace elements, without HEPES and sodium bicarbonate, powder, suitable for cell culture

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别名:
DME/F-12, 1:1混合
MDL编号:
UNSPSC代码:
12352207
NACRES:
NA.71

质量水平

形式

powder

技术

cell culture | mammalian: suitable

组分

NaHCO3: no
sodium pyruvate: 0.11 g/L
HEPES: no
phenol red: yes
glucose: 3.15 g/L
L-glutamine: yes

运输

ambient

储存温度

2-8°C

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一般描述

Dulbecco′s改良Eagle′s培养基/Ham F-12营养混合物是1:1混合物。

应用

Dulbecco′s改良Eagle′s培养基/Ham F-12营养混合物已用于:

  • 小鼠胚胎脑皮质培养
  • 作为人角膜上皮(HCE)培养基组分,培养人角膜缘外植体
  • 人角膜上皮细胞系培养

其他说明

微量元素包括钼酸铵、偏钒酸铵、氯化亚锡、亚硒酸钠、硫酸镁、氯化镍和偏硅酸钠

数量

按照每升培养基12.0克粉末配制。

重悬

补充 1.2 g/L 碳酸氢钠

法规信息

动植物来源培养基

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商品

Nutrient Mixture F-12 Ham Formulation

实验方案

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

粉末培养基及盐混合物极具吸湿性,应注意防潮。

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