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Merck
CN

CY0100

细胞色素C还原酶(NADPH)检测试剂盒

1 kit sufficient for 100 tests, determining cytochrome c reductase activity

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NACRES:
NA.84
UNSPSC Code:
12161503
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产品名称

细胞色素C还原酶(NADPH)检测试剂盒, 1 kit sufficient for 100 tests, determining cytochrome c reductase activity

usage

 kit sufficient for 100 tests

shipped in

dry ice

storage temp.

−20°C

Quality Level

Gene Information

human ... POR(5447)

Analysis Note

细胞色素C还原酶检测试剂盒可快速便捷地检测细胞、组织提取物以及纯化的微粒体中的NADPH-细胞色素C还原酶活性,其含有该检测所需的所有试剂。该试剂盒已通过对肝、肾、脑、脾和心肌等多种哺乳动物组织样本以及HeLa、HepG2和Jurkat等细胞裂解物的测试。此外,还通过了对P. pastoris S. cerevisiae.酵母菌株样本进行的测试。

Biochem/physiol Actions

真核生物的NADPH-细胞色素C还原酶(NADPH-细胞色素P450还原酶,EC1.6.2.4)是一种位于内质网的黄素蛋白。它可将NADPH的电子传递到负责对异型生物质发挥解毒功能的多种氧合酶,其中最重要的是细胞色素P450家族的酶。NADPH-细胞色素C还原酶被广泛用作内质网的标记蛋白,以及生态污染和膳食脂质摄取的生物标记物。

Features and Benefits

  • 用于使用密度梯度分离法在分离和亚细胞分离时检测内质网(ER)
  • 在药物中毒或外源性物质激发后,使用比色法检测多种来源的NADPH-细胞色素C还原酶
  • 用于分析内质网的聚集及其功能

pictograms

Skull and crossbones

signalword

Danger

hcodes

Hazard Classifications

Acute Tox. 3 Oral - Aquatic Chronic 3

存储类别

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

法规信息

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D Plonne et al.
Analytical biochemistry, 276(1), 88-96 (1999-12-10)
A novel method is described for the separation on a single gradient of the major intracellular organelles of the secretory pathway, the Golgi, the smooth endoplasmic reticulum, and the rough endoplasmic reticulum. Total microsomes were prepared from rat liver by
Rohit Sharma et al.
Age (Dordrecht, Netherlands), 36(4), 9686-9686 (2014-07-20)
Imbalance in Th1/Th2 immune pathways and cellular antioxidant systems with progressive aging are among the leading causes of increased risk of morbidity and mortality in elderly. Although probiotics have been considered to boost immune system, there is a lack of
Nathan M Long et al.
Reproductive biology and endocrinology : RB&E, 11, 34-34 (2013-05-10)
Previously we reported decreased circulating progesterone and fertility in one and two year old ewes born to undernourished mothers. This study was designed to investigate if this reduction in progesterone persisted into old age, and if it did, what mechanisms
Diéssica Padilha Dalenogare et al.
Experimental neurology, 328, 113241-113241 (2020-02-12)
Central neuropathic pain is the main symptom caused by spinal cord lesion in relapsing-remitting multiple sclerosis (RRMS), but its management is still not effective. The transient receptor potential ankyrin 1 (TRPA1) is a pain detecting ion channel involved in neuropathic
C O Lemley et al.
Journal of dairy science, 93(3), 1012-1021 (2010-02-23)
In the cow, inadequate concentrations of progesterone during gestation may lead to an abrupt termination of pregnancy. The primary organ involved in progesterone catabolism is the liver, which contains an abundance of cytochrome P450 isozymes (EC 1.14.14.1; mixed-function monooxygenases). The

商品

Centrifugation separates organelles based on size, shape, and density, facilitating subcellular fractionation across various samples.

Cellular apoptosis assays to detect programmed cell death using Annexin V, Caspase and TUNEL DNA fragmentation assays.

细胞凋亡测定使用膜联蛋白V、Caspase和TUNEL DNA片段化检测法检测程序性细胞死亡。

亚细胞组分离心分离是一种常用技术,广泛适用于多种类型的细胞和组织。由于不同细胞器的大小、形状和密度不同,因此通过离心,可从温和均质的样品中轻松分离和纯化细胞器组分。

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