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生化/生理作用
真核生物的NADPH-细胞色素C还原酶（NADPH-细胞色素P450还原酶，EC1.6.2.4）是一种位于内质网的黄素蛋白。它可将NADPH的电子传递到负责对异型生物质发挥解毒功能的多种氧合酶，其中最重要的是细胞色素P450家族的酶。NADPH-细胞色素C还原酶被广泛用作内质网的标记蛋白，以及生态污染和膳食脂质摄取的生物标记物。
特点和优势
- 用于使用密度梯度分离法在分离和亚细胞分离时检测内质网(ER)
- 在药物中毒或外源性物质激发后，使用比色法检测多种来源的NADPH-细胞色素C还原酶
- 用于分析内质网的聚集及其功能
分析说明
细胞色素C还原酶检测试剂盒可快速便捷地检测细胞、组织提取物以及纯化的微粒体中的NADPH-细胞色素C还原酶活性，其含有该检测所需的所有试剂。该试剂盒已通过对肝、肾、脑、脾和心肌等多种哺乳动物组织样本以及HeLa、HepG2和Jurkat等细胞裂解物的测试。此外，还通过了对P. pastoris 和 S. cerevisiae.酵母菌株样本进行的测试。
仅试剂盒组分
产品编号
说明
- Assay Buffer 100 mL
- Enzyme Dilution Buffer 25 mL
- Cytochrome c 50 mg
- Cytochrome c Reductase (NADPH) Positive Control from rabbit liver 50 μL
- Cytochrome c Oxidase Inhibitor Solution 1 mL
试剂盒组分也可单独购买
产品编号
说明
化学品安全说明书
- N6505β-Nicotinamide adenine dinucleotide 2′-phosphate reduced tetrasodium salt hydrate, ≥95% (HPLC) 25 mg化学品安全说明书
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警示用语：
Danger
危险声明
危险分类
Acute Tox. 3 Oral - Aquatic Chronic 3
储存分类代码
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
法规信息
监管及禁止进口产品
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
D Plonne et al.
Analytical biochemistry, 276(1), 88-96 (1999-12-10)
A novel method is described for the separation on a single gradient of the major intracellular organelles of the secretory pathway, the Golgi, the smooth endoplasmic reticulum, and the rough endoplasmic reticulum. Total microsomes were prepared from rat liver by
Nathan M Long et al.
Reproductive biology and endocrinology : RB&E, 11, 34-34 (2013-05-10)
Previously we reported decreased circulating progesterone and fertility in one and two year old ewes born to undernourished mothers. This study was designed to investigate if this reduction in progesterone persisted into old age, and if it did, what mechanisms
Diéssica Padilha Dalenogare et al.
Experimental neurology, 328, 113241-113241 (2020-02-12)
Central neuropathic pain is the main symptom caused by spinal cord lesion in relapsing-remitting multiple sclerosis (RRMS), but its management is still not effective. The transient receptor potential ankyrin 1 (TRPA1) is a pain detecting ion channel involved in neuropathic
Rohit Sharma et al.
Age (Dordrecht, Netherlands), 36(4), 9686-9686 (2014-07-20)
Imbalance in Th1/Th2 immune pathways and cellular antioxidant systems with progressive aging are among the leading causes of increased risk of morbidity and mortality in elderly. Although probiotics have been considered to boost immune system, there is a lack of
C O Lemley et al.
Journal of dairy science, 93(3), 1012-1021 (2010-02-23)
In the cow, inadequate concentrations of progesterone during gestation may lead to an abrupt termination of pregnancy. The primary organ involved in progesterone catabolism is the liver, which contains an abundance of cytochrome P450 isozymes (EC 1.14.14.1; mixed-function monooxygenases). The
商品
亚细胞组分离心分离是一种常用技术，广泛适用于多种类型的细胞和组织。由于不同细胞器的大小、形状和密度不同，因此通过离心，可从温和均质的样品中轻松分离和纯化细胞器组分。
Centrifugation separates organelles based on size, shape, and density, facilitating subcellular fractionation across various samples.
Cellular apoptosis assays to detect programmed cell death using Annexin V, Caspase and TUNEL DNA fragmentation assays.
Learn about the four membrane-bound protein complexes that make up the electron transport chain metabolic pathway supplying energy as ATP for cellular respiration.
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