C9830
壳聚糖酶 来源于灰色链霉菌
lyophilized powder, >50 units/mg protein (Bradford)
别名:
壳聚糖N-乙酰氨基葡糖水解酶
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关于此项目
化学文摘社编号:
UNSPSC Code:
12352204
NACRES:
NA.54
MDL number:
Specific activity:
>50 units/mg protein (Bradford)
form
lyophilized powder
specific activity
>50 units/mg protein (Bradford)
purified by
chromatography
solubility
H2O: soluble, clear to slightly hazy, colorless (10U/0.3mL)
foreign activity
chitinase <1.0 units/mg protein
storage temp.
−20°C
Quality Level
General description
壳聚糖酶作为细胞外分泌酶由灰链霉菌 合成，其分子量为34 kDa。它的最适温度和pH分别为60°C和5.7。壳聚糖酶属于糖基水解酶家族，并包含α-螺旋三级结构，类似于溶菌酶，在两瓣之间存在与底物结合的裂隙。
Application
Sigma的酶已被用于水解卵菌根腐丝囊霉Aphanomyces euteiches（豆科植物的主要寄生虫）的细胞壁粗制品。它还被用于完全脱-N-乙酰化壳聚糖的酶促水解，以便在制备生物相容性壳聚糖-藻酸盐凝胶时得到壳聚糖低聚物混合物。
来自灰链霉菌的壳聚糖酶已被用于消化泪腺（LG）组织。
来自灰色链霉菌（Streptomyces griseus）的壳聚糖酶已被用于评估几丁质来源对几丁质酶和壳聚糖酶产生的影响。来自灰色链霉菌（Streptomyces griseus）的壳聚糖酶还被用于研究：通过使用胶体几丁质和不同程度的壳聚糖脱乙酰化来研究灰色链霉菌（Streptomyces griseus）HUT 6037的几丁质酶和壳聚糖酶的有效产生。
Biochem/physiol Actions
壳聚糖酶可在N-乙酰-D-氨基葡萄糖和D-氨基葡萄糖残基之间的β（1,4）键处催化N-乙酰化壳聚糖的内水解。来自灰链霉菌的壳聚糖酶能够水解壳聚糖和羧甲基纤维素。壳聚糖酶存在于多种类型的微生物中，并可用于真菌细胞壁的裂解。
Physical form
含磷酸钾缓冲盐的冻干粉
Preparation Note
对于长期溶液储存，应使用50％甘油溶液重悬样品瓶内容物。用0.1％BSA溶液稀释至工作浓度。
Other Notes
根据Osswald等(1992)描述的荧光测定法，一个酶活性单位是指在pH 5.0、37℃下，每分钟催化壳聚糖释放1 μmole葡萄糖胺所需的酶量。
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
存储类别
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
常规特殊物品
此项目有
Ilham Badreddine et al.
Eukaryotic cell, 7(11), 1980-1993 (2008-09-23)
Chitin is an essential component of fungal cell walls, where it forms a crystalline scaffold, and chitooligosaccharides derived from it are signaling molecules recognized by the hosts of pathogenic fungi. Oomycetes are cellulosic fungus-like microorganisms which most often lack chitin
Regulating temporospatial dynamics of morphogen for structure formation of the lacrimal gland by chitosan biomaterials
Hsiao YC and Yang TL
Biomaterials, 113, 42-55 (2017)
Effective production of chitinase and chitosanase by Streptomyces griseus HUT 6037 using colloidal chitin and various degrees of deacetylation of chitosan
Jung HS, et al.
Biotechnology and Bioprocess Engineering: BBE, 4(1), 26-31 (1999)
Chitosanase from Streptomyces griseus.
A Ohtakara
Methods in enzymology, 161, 505-510 (1988-01-01)
Thang Trung Khong et al.
Biomacromolecules, 14(8), 2765-2771 (2013-06-29)
Biocompatible hydrogels are very interesting for applications in, e.g., tissue engineering and for immobilization of cells, such as calcium-alginate gels where the calcium ions form specific interactions with the guluronic acid units. We here report on a new gelling system
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